##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BalI BbeI BcgI
BclI BfrI BglII BsaBI BsaHI
BsiEI BsiWI Bsp1407I BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI SacII SalI ScaI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2069 GTCTAC GT^[AC][GT]AC
AccIII 1713 TCCGGA T^CCGGA
AcsI 2409 GAATTT [GA]^AATT[CT]
Aor51HI 1339 AGCGCT AGC^GCT
ApoI 2409 GAATTT [GA]^AATT[CT]
AvrII 156 CCTAGG C^CTAGG
BamHI 1710 GGATCC G^GATCC
BbvCI 1066 CCTCAGC CCTCAGC(-5/-2)
BciVI 720 GTATCC GTATCC(6/5)
BlnI 156 CCTAGG C^CTAGG
BsaI 2703 GGTCTC GGTCTC(1/5)
BseAI 1713 TCCGGA T^CCGGA
BsmBI 1537 CGTCTC CGTCTC(1/5)
BsmI 2468 GAATGC GAATGC(1/-1)
BspDI 1512 ATCGAT AT^CGAT
BspEI 1713 TCCGGA T^CCGGA
BsrBI 49 GAGCGG CCGCTC(-3/-3)
BssSI 1183 CTCGTG CACGAG(-5/-1)
ClaI 1512 ATCGAT AT^CGAT
CpoI 1613 CGGACCG CG^G[AT]CCG
Eco47III 1339 AGCGCT AGC^GCT
HincII 986 GTTGAC GT[CT]^[GA]AC
HindII 986 GTTGAC GT[CT]^[GA]AC
MroI 1713 TCCGGA T^CCGGA
PaeR7I 2610 CTCGAG C^TCGAG
RsrII 1613 CGGACCG CG^G[AT]CCG
SapI 2631 GCTCTTC GCTCTTC(1/4)
SbfI 2965 CCTGCAGG CCTGCA^GG
SexAI 1038 ACCAGGT A^CC[AT]GGT
SfiI 577 GGCCAGAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2965 CCTGCAGG CCTGCA^GG
SspI 3414 AATATT AAT^ATT
TfiI 3206 GATTC G^A[AT]TC
XbaI 628 TCTAGA T^CTAGA
XhoI 2610 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbrPI 944 CACGTG CAC^GTG
3106 CACGTG
BglI 578 GCCAGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3278 GCCCCTGAGGC
BsaWI 1713 TCCGGA [AT]^CCGG[AT]
3193 TCCGGT
BstAPI 1882 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2989 GCAGCCCCTGC
BstXI 263 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2892 CCAGGAAAGTGG
DraIII 1676 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3103 CACCACGTG
DrdI 681 GACGGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1055 GACTTTACAGTC
EcoNI 151 CCTTGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2962 CCTCCTGCAGG
HaeII 1339 AGCGCT [GA]GCGC^[CT]
3002 AGCGCC
NcoI 1230 CCATGG C^CATGG
1647 CCATGG
NspI 348 GCATGC [GA]CATG^[CT]
2182 GCATGC
PflMI 1627 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2819 CCAGCTGGTGG
PmaCI 944 CACGTG CAC^GTG
3106 CACGTG
PmlI 944 CACGTG CAC^GTG
3106 CACGTG
PshAI 227 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
795 GACTCTTGTC
SacI 1167 GAGCTC GAGCT^C
1925 GAGCTC
SmaI 1937 CCCGGG CCC^GGG
2733 CCCGGG
SphI 348 GCATGC GCATG^C
2182 GCATGC
Van91I 1627 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2819 CCAGCTGGTGG
XcmI 2097 CCAGAGCACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2553 CCACGCCGCCCTTGG
XmaCI 1937 CCCGGG C^CCGGG
2733 CCCGGG
XmaI 1937 CCCGGG C^CCGGG
2733 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII