##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
BbeI BcgI BclI BfrI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 1107 GACGCC G[GA]^CG[CT]C
Alw44I 1242 GTGCAC G^TGCAC
ApaLI 1242 GTGCAC G^TGCAC
AspI 633 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 575 CCTAGG C^CTAGG
BamHI 3026 GGATCC G^GATCC
BbrPI 2173 CACGTG CAC^GTG
BbvCI 2231 GCTGAGG CCTCAGC(-5/-2)
BciVI 1652 GTATCC GTATCC(6/5)
BlnI 575 CCTAGG C^CTAGG
BsaHI 1107 GACGCC G[GA]^CG[CT]C
BsaI 939 GAGACC GGTCTC(1/5)
BsaWI 1476 TCCGGT [AT]^CCGG[AT]
BsmBI 2711 GAGACG CGTCTC(1/5)
BspMI 962 GCAGGT ACCTGC(4/8)
BsrFI 2613 GCCGGT [GA]^CCGG[CT]
BssSI 2855 CTCGTG CACGAG(-5/-1)
BstEII 112 GGTGACC G^GT[ACGT]ACC
BstPI 112 GGTGACC G^GT[ACGT]ACC
Cfr10I 2613 GCCGGT [GA]^CCGG[CT]
DraIII 2742 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 112 GGTGACC G^GT[ACGT]ACC
Hin1I 1107 GACGCC G[GA]^CG[CT]C
HindIII 2820 AAGCTT A^AGCTT
NdeI 983 CATATG CA^TATG
NspI 2151 GCATGC [GA]CATG^[CT]
PflFI 633 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2173 CACGTG CAC^GTG
PmlI 2173 CACGTG CAC^GTG
PvuII 1914 CAGCTG CAG^CTG
SapI 2237 GAAGAGC GCTCTTC(1/4)
SmaI 2563 CCCGGG CCC^GGG
SphI 2151 GCATGC GCATG^C
Tth111I 633 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2563 CCCGGG C^CCGGG
XmaI 2563 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 733 GTAGAC GT^[AC][GT]AC
1480 GTAGAC
AcsI 523 AAATTC [GA]^AATT[CT]
3178 AAATTT
ApoI 523 AAATTC [GA]^AATT[CT]
3178 AAATTT
Asp700I 740 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
913 GAACTGCTTC
BalI 781 TGGCCA TGG^CCA
1640 TGGCCA
BglI 170 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
980 GCCCATATGGC
BglII 1120 AGATCT A^GATCT
2621 AGATCT
BlpI 17 GCTCAGC GC^T[ACGT]AGC
2146 GCTGAGC
Bpu1102I 17 GCTCAGC GC^T[ACGT]AGC
2146 GCTGAGC
BsmI 43 GCATTC GAATGC(1/-1)
2338 GCATTC
CelII 17 GCTCAGC GC^T[ACGT]AGC
2146 GCTGAGC
DsaI 502 CCATGG C^C[GA][CT]GG
1637 CCATGG
EcoNI 924 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3038 CCTATGGAAGG
MluNI 781 TGGCCA TGG^CCA
1640 TGGCCA
MscI 781 TGGCCA TGG^CCA
1640 TGGCCA
MseI 1383 TTAA T^TAA
2385 TTAA
MspA1I 1914 CAGCTG C[AC]G^C[GT]G
2953 CAGCGG
NcoI 502 CCATGG C^CATGG
1637 CCATGG
SacI 15 GAGCTC GAGCT^C
1341 GAGCTC
TfiI 1280 GATTC G^A[AT]TC
3134 GATTC
Tru9I 1383 TTAA T^TAA
2385 TTAA
XcmI 1942 CCAAATAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2172 CCACGTGGGACCTGG
XmnI 740 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
913 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
AviII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII