##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I ApaLI AscI Asp700I
AspI AviII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstZ17I Cfr10I ClaI CpoI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2733 GGTACC G^GTACC
AhdI 408 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 130 GATCC GGATC(4/5)
Aor51HI 69 AGCGCT AGC^GCT
AseI 652 ATTAAT AT^TAAT
AsnI 652 ATTAAT AT^TAAT
Asp718I 2733 GGTACC G^GTACC
AspEI 408 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 685 GTCTTC GAAGAC(2/6)
BglI 2070 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2395 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2395 GCTAAGC GC^T[ACGT]AGC
BpuAI 685 GTCTTC GAAGAC(2/6)
BsmI 1261 GCATTC GAATGC(1/-1)
BspHI 2339 TCATGA T^CATGA
BstAPI 1271 GCAACAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2373 TTCGAA TT^CGAA
BstEII 2816 GGTCACC G^GT[ACGT]ACC
BstPI 2816 GGTCACC G^GT[ACGT]ACC
BstXI 1122 CCACTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2395 GCTAAGC GC^T[ACGT]AGC
Eam1105I 408 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 69 AGCGCT AGC^GCT
EcoO65I 2816 GGTCACC G^GT[ACGT]ACC
HincII 471 GTTGAC GT[CT]^[GA]AC
HindII 471 GTTGAC GT[CT]^[GA]AC
KpnI 2733 GGTACC GGTAC^C
NspV 2373 TTCGAA TT^CGAA
PflMI 634 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 652 ATTAAT AT^TAAT
PvuII 2907 CAGCTG CAG^CTG
RcaI 2339 TCATGA T^CATGA
SexAI 1358 ACCTGGT A^CC[AT]GGT
SfuI 2373 TTCGAA TT^CGAA
StuI 386 AGGCCT AGG^CCT
Van91I 634 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 1515 CGCG CG^CG
1878 CGCG
AcyI 1086 GGCGTC G[GA]^CG[CT]C
1468 GACGCC
AflIII 896 ACATGT A^C[GA][CT]GT
2190 ACATGT
AlwNI 1171 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1444 CAGCGGCTG
ApaI 1246 GGGCCC GGGCC^C
2617 GGGCCC
AvrII 594 CCTAGG C^CTAGG
1716 CCTAGG
BanI 1457 GGTGCC G^G[CT][GA]CC
2733 GGTACC
BbvCI 72 GCTGAGG CCTCAGC(-5/-2)
1176 GCTGAGG
BlnI 594 CCTAGG C^CTAGG
1716 CCTAGG
BsaHI 1086 GGCGTC G[GA]^CG[CT]C
1468 GACGCC
BspLU11I 896 ACATGT A^CATGT
2190 ACATGT
BspMI 981 GCAGGT ACCTGC(4/8)
1389 GCAGGT
BsrDI 459 CATTGC GCAATG(2/0)
2198 CATTGC
BstUI 1515 CGCG CG^CG
1878 CGCG
DraIII 440 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1495 CACGCCGTG
DrdI 268 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1354 GACCACCTGGTC
DsaI 1499 CCGTGG C^C[GA][CT]GG
1608 CCACGG
HaeII 69 AGCGCT [GA]GCGC^[CT]
1284 AGCGCC
HgaI 1468 GACGC GACGC(5/10)
1087 GCGTC
Hin1I 1086 GGCGTC G[GA]^CG[CT]C
1468 GACGCC
MaeII 863 ACGT A^CGT
1304 ACGT
MspA1I 1444 CAGCGG C[AC]G^C[GT]G
2907 CAGCTG
MvnI 1515 CGCG CG^CG
1878 CGCG
PstI 1241 CTGCAG CTGCA^G
2783 CTGCAG
SapI 1641 GCTCTTC GCTCTTC(1/4)
1721 GCTCTTC
TaqI 2374 TCGA T^CGA
2482 TCGA
TthHB8I 2374 TCGA T^CGA
2482 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AocI
ApoI AspHI AvaI AvaII BalI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmaCI XmaI