##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI Asp718I AspEI
AspI AviII AvrII BamHI BciVI
BfrI BglI BlnI BsaBI BseAI
BsiEI BspDI BspEI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MamI MluI MroI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 2386 GTCTAC GT^[AC][GT]AC
AcsI 998 AAATTC [GA]^AATT[CT]
AcyI 2794 GGCGCC G[GA]^CG[CT]C
Aor51HI 3185 AGCGCT AGC^GCT
ApaI 3457 GGGCCC GGGCC^C
ApoI 998 AAATTC [GA]^AATT[CT]
AseI 2715 ATTAAT AT^TAAT
AsnI 2715 ATTAAT AT^TAAT
Asp700I 1522 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 545 TGGCCA TGG^CCA
BbeI 2794 GGCGCC GGCGC^C
BbrPI 1783 CACGTG CAC^GTG
BbsI 1299 GAAGAC GAAGAC(2/6)
BbvCI 1696 GCTGAGG CCTCAGC(-5/-2)
BcgI 1739 GCAAAACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 659 TGATCA T^GATCA
BpuAI 1299 GAAGAC GAAGAC(2/6)
BsaAI 1783 CACGTG [CT]AC^GT[GA]
BsaHI 2794 GGCGCC G[GA]^CG[CT]C
BsaWI 1590 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2944 TGTACA T^GTACA
BsrBI 673 GAGCGG CCGCTC(-3/-3)
BsrDI 393 CATTGC GCAATG(2/0)
BsrFI 2288 GCCGGC [GA]^CCGG[CT]
BsrGI 2944 TGTACA T^GTACA
BssHII 1489 GCGCGC G^CGCGC
Cfr10I 2288 GCCGGC [GA]^CCGG[CT]
DraIII 3012 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3185 AGCGCT AGC^GCT
EcoRV 2002 GATATC GAT^ATC
FbaI 659 TGATCA T^GATCA
Hin1I 2794 GGCGCC G[GA]^CG[CT]C
KasI 2794 GGCGCC G^GCGCC
MfeI 794 CAATTG C^AATTG
MluNI 545 TGGCCA TGG^CCA
MscI 545 TGGCCA TGG^CCA
MunI 794 CAATTG C^AATTG
NaeI 2288 GCCGGC GCC^GGC
NarI 2794 GGCGCC GG^CGCC
NgoMIV 2288 GCCGGC G^CCGGC
PaeR7I 1480 CTCGAG C^TCGAG
PmaCI 1783 CACGTG CAC^GTG
PmlI 1783 CACGTG CAC^GTG
PshAI 333 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2715 ATTAAT AT^TAAT
SfoI 2794 GGCGCC GGC^GCC
SmaI 2434 CCCGGG CCC^GGG
SspBI 2944 TGTACA T^GTACA
XbaI 1530 TCTAGA T^CTAGA
XcmI 3146 CCACAGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1480 CTCGAG C^TCGAG
XmaCI 2434 CCCGGG C^CCGGG
XmaI 2434 CCCGGG C^CCGGG
XmnI 1522 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1466 CGCG CG^CG
1490 CGCG
Alw44I 2803 GTGCAC G^TGCAC
2969 GTGCAC
AocI 2212 CCTCAGG CC^T[ACGT]AGG
2586 CCTAAGG
ApaLI 2803 GTGCAC G^TGCAC
2969 GTGCAC
BglII 377 AGATCT A^GATCT
3317 AGATCT
BlpI 31 GCTCAGC GC^T[ACGT]AGC
1661 GCTCAGC
Bpu1102I 31 GCTCAGC GC^T[ACGT]AGC
1661 GCTCAGC
BsgI 3252 CTGCAC GTGCAG(16/14)
1324 GTGCAG
BsiWI 398 CGTACG C^GTACG
956 CGTACG
BsmBI 2254 CGTCTC CGTCTC(1/5)
2661 CGTCTC
BspLU11I 2973 ACATGT A^CATGT
3256 ACATGT
BssSI 1417 CACGAG CACGAG(-5/-1)
2934 CTCGTG
BstUI 1466 CGCG CG^CG
1490 CGCG
BstXI 944 CCAAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2340 CCATGCAGGTGG
Bsu36I 2212 CCTCAGG CC^T[ACGT]AGG
2586 CCTAAGG
CelII 31 GCTCAGC GC^T[ACGT]AGC
1661 GCTCAGC
DraI 519 TTTAAA TTT^AAA
1536 TTTAAA
EaeI 545 TGGCCA [CT]^GGCC[GA]
2883 TGGCCG
Eco81I 2212 CCTCAGG CC^T[ACGT]AGG
2586 CCTAAGG
HgaI 538 GACGC GACGC(5/10)
1434 GCGTC
HincII 1825 GTCAAC GT[CT]^[GA]AC
2494 GTTGAC
HindII 1825 GTCAAC GT[CT]^[GA]AC
2494 GTTGAC
MvnI 1466 CGCG CG^CG
1490 CGCG
NspI 2973 ACATGT [GA]CATG^[CT]
3256 ACATGT
SapI 325 GAAGAGC GCTCTTC(1/4)
670 GAAGAGC
SexAI 1853 ACCTGGT A^CC[AT]GGT
3094 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII