##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AccIII AclI AscI AseI
AsnI Asp700I AvrII BamHI BbrPI
BcgI BlnI BsaAI BsaBI BseAI
BsiWI BspEI BssHII Bst1107I BstZ17I
CpoI EcoT22I FseI HpaI MamI
MfeI MluI MroI MunI NotI
NruI NsiI PacI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SexAI SfiI SgrAI SnaBI
SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1186 GGTACC G^GTACC
AgeI 1522 ACCGGT A^CCGGT
Aor51HI 8580 AGCGCT AGC^GCT
Asp718I 1186 GGTACC G^GTACC
AviII 1827 TGCGCA TGC^GCA
BbeI 1165 GGCGCC GGCGC^C
BciVI 8801 GGATAC GTATCC(6/5)
BclI 9003 TGATCA T^GATCA
BspDI 3656 ATCGAT AT^CGAT
BstBI 2681 TTCGAA TT^CGAA
BstEII 6920 GGTGACC G^GT[ACGT]ACC
BstPI 6920 GGTGACC G^GT[ACGT]ACC
ClaI 3656 ATCGAT AT^CGAT
DrdI 1597 GACTGATTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 8580 AGCGCT AGC^GCT
EcoO65I 6920 GGTGACC G^GT[ACGT]ACC
EcoRI 1635 GAATTC G^AATTC
EcoRV 8212 GATATC GAT^ATC
FbaI 9003 TGATCA T^GATCA
FspI 1827 TGCGCA TGC^GCA
KasI 1165 GGCGCC G^GCGCC
KpnI 1186 GGTACC GGTAC^C
KspI 6925 CCGCGG CCGC^GG
NarI 1165 GGCGCC GG^CGCC
NdeI 9661 CATATG CA^TATG
NheI 8954 GCTAGC G^CTAGC
NspV 2681 TTCGAA TT^CGAA
PinAI 1522 ACCGGT A^CCGGT
PshAI 5341 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 6925 CCGCGG CCGC^GG
SbfI 8964 CCTGCAGG CCTGCA^GG
ScaI 3646 AGTACT AGT^ACT
SfoI 1165 GGCGCC GGC^GCC
SfuI 2681 TTCGAA TT^CGAA
Sse8387I 8964 CCTGCAGG CCTGCA^GG
XbaI 8772 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 8996 CTTAAG C^TTAAG
9178 CTTAAG
AflIII 3730 ACATGT A^C[GA][CT]GT
5886 ACATGT
BfrI 8996 CTTAAG C^TTAAG
9178 CTTAAG
BglII 2098 AGATCT A^GATCT
5967 AGATCT
BspHI 647 TCATGA T^CATGA
2044 TCATGA
BspLU11I 3730 ACATGT A^CATGT
5886 ACATGT
DraI 6217 TTTAAA TTT^AAA
9258 TTTAAA
DraIII 1773 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
6424 CACAAAGTG
EagI 808 CGGCCG C^GGCCG
2692 CGGCCG
EclXI 808 CGGCCG C^GGCCG
2692 CGGCCG
Eco52I 808 CGGCCG C^GGCCG
2692 CGGCCG
MaeII 202 ACGT A^CGT
1313 ACGT
NaeI 1052 GCCGGC GCC^GGC
3670 GCCGGC
NgoMIV 1052 GCCGGC G^CCGGC
3670 GCCGGC
PvuII 264 CAGCTG CAG^CTG
676 CAGCTG
RcaI 647 TCATGA T^CATGA
2044 TCATGA
SpeI 1451 ACTAGT A^CTAGT
9491 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PaeR7I PflFI PflMI
PleI PpuMI PstI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI