##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccII AclI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AvaI
AviII AvrII BbeI BbrPI BcgI
BfrI BglI BlnI BsaBI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BsrBI BsrGI BssHII BssSI BstEII
BstPI BstUI ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI FspI HaeII HgaI
KasI KspI MamI MluI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SmaI SnaBI Sse8387I SspBI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1989 GACGTC GACGT^C
Acc65I 1432 GGTACC G^GTACC
AccI 4275 GTATAC GT^[AC][GT]AC
AccIII 1213 TCCGGA T^CCGGA
AcyI 1989 GACGTC G[GA]^CG[CT]C
AhdI 3217 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1432 GGTACC G^GTACC
AspEI 3217 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5632 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4169 GGATCC G^GATCC
BbvCI 540 GCTGAGG CCTCAGC(-5/-2)
BcnI 1784 CCCGG CC^[GC]GG
BglII 2255 AGATCT A^GATCT
BsaAI 3847 CACGTA [CT]AC^GT[GA]
BsaHI 1989 GACGTC G[GA]^CG[CT]C
BseAI 1213 TCCGGA T^CCGGA
BseRI 90 CTCCTC GAGGAG(10/8)
BsgI 3271 GTGCAG GTGCAG(16/14)
BspEI 1213 TCCGGA T^CCGGA
BsrDI 3333 CATTGC GCAATG(2/0)
BsrFI 3551 GCCGGT [GA]^CCGG[CT]
Bst1107I 4275 GTATAC GTA^TAC
BstBI 1892 TTCGAA TT^CGAA
BstZ17I 4275 GTATAC GTA^TAC
Cfr10I 3551 GCCGGT [GA]^CCGG[CT]
DrdI 3606 GACCCGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3217 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1729 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1645 GAATTC G^AATTC
FauI 3608 CCCGC CCCGC(4/6)
Hin1I 1989 GACGTC G[GA]^CG[CT]C
HincII 5166 GTTAAC GT[CT]^[GA]AC
HindII 5166 GTTAAC GT[CT]^[GA]AC
HpaI 5166 GTTAAC GTT^AAC
KpnI 1432 GGTACC GGTAC^C
MroI 1213 TCCGGA T^CCGGA
NciI 1784 CCCGG CC^[GC]GG
NspV 1892 TTCGAA TT^CGAA
PflFI 5632 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 431 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1200 GAGCTC GAGCT^C
SapI 1197 GAAGAGC GCTCTTC(1/4)
SfuI 1892 TTCGAA TT^CGAA
SgrAI 3550 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 1824 ACTAGT A^CTAGT
SphI 4224 GCATGC GCATG^C
Tth111I 5632 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 4244 ACATGT A^C[GA][CT]GT
4853 ACATGT
BanII 1200 GAGCTC G[GA]GC[CT]^C
1739 GGGCTC
BbsI 1518 GAAGAC GAAGAC(2/6)
3316 GTCTTC
BciVI 3285 GGATAC GTATCC(6/5)
256 GTATCC
BclI 3540 TGATCA T^GATCA
4487 TGATCA
BpuAI 1518 GAAGAC GAAGAC(2/6)
3316 GTCTTC
BsaWI 1213 TCCGGA [AT]^CCGG[AT]
1328 ACCGGA
BspHI 1451 TCATGA T^CATGA
1641 TCATGA
BspLU11I 4244 ACATGT A^CATGT
4853 ACATGT
BstAPI 1354 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2082 GCATAAGATGC
BstXI 1616 CCATTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3245 CCACATCTTTGG
CfoI 1697 GCGC GCG^C
3994 GCGC
FbaI 3540 TGATCA T^GATCA
4487 TGATCA
HhaI 1697 GCGC GCG^C
3994 GCGC
HinP1I 1697 GCGC G^CGC
3994 GCGC
HindIII 1915 AAGCTT A^AGCTT
4730 AAGCTT
MfeI 4103 CAATTG C^AATTG
5192 CAATTG
MunI 4103 CAATTG C^AATTG
5192 CAATTG
NcoI 3011 CCATGG C^CATGG
3239 CCATGG
NdeI 4865 CATATG CA^TATG
4919 CATATG
RcaI 1451 TCATGA T^CATGA
1641 TCATGA
ScaI 1077 AGTACT AGT^ACT
2066 AGTACT
SexAI 137 ACCTGGT A^CC[AT]GGT
387 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI