##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI BclI BfrI BglII BseAI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FbaI FseI HindIII
HpaI KspI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PinAI PmeI PshBI
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 4743 ACACGT A^C[GA][CT]GT
Asp700I 241 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3871 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3916 CCTAGG C^CTAGG
BamHI 2882 GGATCC G^GATCC
BbeI 4549 GGCGCC GGCGC^C
BcgI 1536 GCACGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3916 CCTAGG C^CTAGG
BsaBI 4587 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1416 TCCGGT [AT]^CCGG[AT]
BsiEI 4338 CGACCG CG[GA][CT]^CG
EcoT22I 4621 ATGCAT ATGCA^T
HaeII 4549 GGCGCC [GA]GCGC^[CT]
KasI 4549 GGCGCC G^GCGCC
MamI 4587 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1620 GCCGGC GCC^GGC
NarI 4549 GGCGCC GG^CGCC
NcoI 4553 CCATGG C^CATGG
NgoMIV 1620 GCCGGC G^CCGGC
NsiI 4621 ATGCAT ATGCA^T
PaeR7I 397 CTCGAG C^TCGAG
PflFI 3871 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 4510 GAGCTC GAGCT^C
SbfI 5612 CCTGCAGG CCTGCA^GG
SfoI 4549 GGCGCC GGC^GCC
SphI 3122 GCATGC GCATG^C
Sse8387I 5612 CCTGCAGG CCTGCA^GG
Tth111I 3871 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 397 CTCGAG C^TCGAG
XmnI 241 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2201 GGTACC G^GTACC
2675 GGTACC
AclI 2158 AACGTT AA^CGTT
2632 AACGTT
AocI 87 CCTCAGG CC^T[ACGT]AGG
3040 CCTCAGG
ApaI 1900 GGGCCC GGGCC^C
2374 GGGCCC
Asp718I 2201 GGTACC G^GTACC
2675 GGTACC
BbrPI 2002 CACGTG CAC^GTG
2476 CACGTG
BciVI 2042 GTATCC GTATCC(6/5)
2516 GTATCC
BglI 988 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1332 GCCTGAAAGGC
BsmI 2055 GCATTC GAATGC(1/-1)
2529 GCATTC
Bsp1407I 4292 TGTACA T^GTACA
4913 TGTACA
BsrBI 4990 CCGCTC CCGCTC(-3/-3)
5035 GAGCGG
BsrGI 4292 TGTACA T^GTACA
4913 TGTACA
BssSI 2937 CTCGTG CACGAG(-5/-1)
4909 CTCGTG
BstAPI 2919 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3501 GCAGGAGCTGC
Bsu36I 87 CCTCAGG CC^T[ACGT]AGG
3040 CCTCAGG
DraIII 34 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3227 CACAGAGTG
Eco81I 87 CCTCAGG CC^T[ACGT]AGG
3040 CCTCAGG
EcoRI 344 GAATTC G^AATTC
3133 GAATTC
HincII 759 GTCAAC GT[CT]^[GA]AC
2842 GTTGAC
HindII 759 GTCAAC GT[CT]^[GA]AC
2842 GTTGAC
KpnI 2201 GGTACC GGTAC^C
2675 GGTACC
PmaCI 2002 CACGTG CAC^GTG
2476 CACGTG
PmlI 2002 CACGTG CAC^GTG
2476 CACGTG
PshAI 3359 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3796 GACTCGGGTC
Psp1406I 2158 AACGTT AA^CGTT
2632 AACGTT
SapI 371 GAAGAGC GCTCTTC(1/4)
2769 GAAGAGC
SspBI 4292 TGTACA T^GTACA
4913 TGTACA
XbaI 738 TCTAGA T^CTAGA
5591 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI