##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccIII Aor51HI AscI AseI
AsnI Asp718I AviII AvrII BciVI
BclI BlnI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eco47III EcoRI EcoRV
FbaI FspI HindIII HpaI KpnI
KspI MamI MluI MroI NdeI
NotI NspV PacI PaeR7I PmeI
PshBI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 926 GACGTC GACGT^C
AclI 5043 AACGTT AA^CGTT
AflII 3774 CTTAAG C^TTAAG
AflIII 26 ACGTGT A^C[GA][CT]GT
AhdI 3969 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1403 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3969 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 185 GGCGCC GGCGC^C
BfrI 3774 CTTAAG C^TTAAG
BglII 996 AGATCT A^GATCT
BsrDI 5065 GCAATG GCAATG(2/0)
BssHII 5 GCGCGC G^CGCGC
BssSI 4228 CACGAG CACGAG(-5/-1)
EagI 103 CGGCCG C^GGCCG
Eam1105I 3969 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 103 CGGCCG C^GGCCG
Eco52I 103 CGGCCG C^GGCCG
EcoT22I 3101 ATGCAT ATGCA^T
FseI 1436 GGCCGGCC GGCCGG^CC
KasI 185 GGCGCC G^GCGCC
MfeI 3281 CAATTG C^AATTG
MunI 3281 CAATTG C^AATTG
NaeI 1437 GCCGGC GCC^GGC
NarI 185 GGCGCC GG^CGCC
NgoMIV 1437 GCCGGC G^CCGGC
NruI 4803 TCGCGA TCG^CGA
NsiI 3101 ATGCAT ATGCA^T
Psp1406I 5043 AACGTT AA^CGTT
SacI 4220 GAGCTC GAGCT^C
SexAI 4245 ACCAGGT A^CC[AT]GGT
SfiI 3912 GGCCATGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 185 GGCGCC GGC^GCC
SmaI 669 CCCGGG CCC^GGG
SphI 894 GCATGC GCATG^C
XbaI 1483 TCTAGA T^CTAGA
XcmI 4996 CCAGCCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 669 CCCGGG C^CCGGG
XmaI 669 CCCGGG C^CCGGG
XmnI 1403 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 92 GTCTAC GT^[AC][GT]AC
3966 GTAGAC
AcsI 1872 AAATTC [GA]^AATT[CT]
2293 GAATTT
AgeI 677 ACCGGT A^CCGGT
692 ACCGGT
Alw44I 1226 GTGCAC G^TGCAC
5415 GTGCAC
ApaI 79 GGGCCC GGGCC^C
987 GGGCCC
ApaLI 1226 GTGCAC G^TGCAC
5415 GTGCAC
ApoI 1872 AAATTC [GA]^AATT[CT]
2293 GAATTT
BcgI 643 CGAGGGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1986 GCAGGGGACTCG
BsiEI 103 CGGCCG CG[GA][CT]^CG
679 CGGTCG
BsmI 4060 GCATTC GAATGC(1/-1)
5239 GCATTC
BstEII 673 GGTGACC G^GT[ACGT]ACC
804 GGTCACC
BstPI 673 GGTGACC G^GT[ACGT]ACC
804 GGTCACC
DraIII 1132 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2370 CACTGGGTG
EcoNI 2851 CCTAGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3936 CCTGGTGAAGG
EcoO65I 673 GGTGACC G^GT[ACGT]ACC
804 GGTCACC
HincII 1423 GTTGAC GT[CT]^[GA]AC
4618 GTTGAC
HindII 1423 GTTGAC GT[CT]^[GA]AC
4618 GTTGAC
NcoI 45 CCATGG C^CATGG
429 CCATGG
NheI 2715 GCTAGC G^CTAGC
4330 GCTAGC
PinAI 677 ACCGGT A^CCGGT
692 ACCGGT
SapI 401 GAAGAGC GCTCTTC(1/4)
482 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII