##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AccIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp700I AspEI AviII BciVI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI FspI HindIII HpaI KspI
MluI MroI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccII 5621 CGCG CG^CG
AclI 1570 AACGTT AA^CGTT
Aor51HI 5365 AGCGCT AGC^GCT
AspI 4040 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 176 GGCGCC GGCGC^C
BsaBI 436 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 3689 GTGCAG GTGCAG(16/14)
BsrBI 85 GAGCGG CCGCTC(-3/-3)
BstUI 5621 CGCG CG^CG
DrdI 3328 GACTGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5365 AGCGCT AGC^GCT
EcoRI 983 GAATTC G^AATTC
HincII 3734 GTCAAC GT[CT]^[GA]AC
HindII 3734 GTCAAC GT[CT]^[GA]AC
KasI 176 GGCGCC G^GCGCC
MamI 436 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 27 CAATTG C^AATTG
MunI 27 CAATTG C^AATTG
MvnI 5621 CGCG CG^CG
NaeI 477 GCCGGC GCC^GGC
NarI 176 GGCGCC GG^CGCC
NgoMIV 477 GCCGGC G^CCGGC
PflFI 4040 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4505 CCAAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1570 AACGTT AA^CGTT
SfiI 678 GGCCTCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 176 GGCGCC GGC^GCC
SspI 5825 AATATT AAT^ATT
Tth111I 4040 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4505 CCAAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 62 GGTACC G^GTACC
5580 GGTACC
Asp718I 62 GGTACC G^GTACC
5580 GGTACC
AvrII 766 CCTAGG C^CTAGG
5357 CCTAGG
BbrPI 3559 CACGTG CAC^GTG
5265 CACGTG
BbsI 1044 GTCTTC GAAGAC(2/6)
5474 GTCTTC
BcgI 2298 CGAGGTAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
306 GCAACTGACTCG
BclI 2954 TGATCA T^GATCA
4842 TGATCA
BglII 3847 AGATCT A^GATCT
4583 AGATCT
BlnI 766 CCTAGG C^CTAGG
5357 CCTAGG
BpuAI 1044 GTCTTC GAAGAC(2/6)
5474 GTCTTC
BsmBI 5295 CGTCTC CGTCTC(1/5)
120 GAGACG
BsmI 2114 GAATGC GAATGC(1/-1)
3795 GCATTC
Bsp1407I 734 TGTACA T^GTACA
3242 TGTACA
BsrFI 139 ACCGGC [GA]^CCGG[CT]
477 GCCGGC
BsrGI 734 TGTACA T^GTACA
3242 TGTACA
Cfr10I 139 ACCGGC [GA]^CCGG[CT]
477 GCCGGC
DraI 1915 TTTAAA TTT^AAA
5837 TTTAAA
EarI 2938 GAAGAG CTCTTC(1/4)
4565 GAAGAG
EcoT22I 1863 ATGCAT ATGCA^T
5544 ATGCAT
FbaI 2954 TGATCA T^GATCA
4842 TGATCA
KpnI 62 GGTACC GGTAC^C
5580 GGTACC
Ksp632I 2938 GAAGAG CTCTTC(1/4)
4565 GAAGAG
NcoI 4726 CCATGG C^CATGG
4741 CCATGG
NdeI 1866 CATATG CA^TATG
1892 CATATG
NsiI 1863 ATGCAT ATGCA^T
5544 ATGCAT
PmaCI 3559 CACGTG CAC^GTG
5265 CACGTG
PmlI 3559 CACGTG CAC^GTG
5265 CACGTG
SacI 3620 GAGCTC GAGCT^C
5254 GAGCTC
SmaI 404 CCCGGG CCC^GGG
3422 CCCGGG
SphI 2238 GCATGC GCATG^C
4149 GCATGC
SspBI 734 TGTACA T^GTACA
3242 TGTACA
XbaI 1849 TCTAGA T^CTAGA
4078 TCTAGA
XmaCI 404 CCCGGG C^CCGGG
3422 CCCGGG
XmaI 404 CCCGGG C^CCGGG
3422 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvCI
BbvI BcnI BfaI BfrI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII