##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspI AviII BamHI
BanI BbeI BbrPI BcgI BcnI
BsaBI BsaHI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstBI BstEII
BstPI BstUI Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI HaeII HgaI
Hin1I KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PflFI PinAI PmaCI PmeI
PmlI PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SpeI Sse8387I SspBI StuI
SwaI Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 4146 GTATAC GT^[AC][GT]AC
AclI 198 AACGTT AA^CGTT
AhdI 1258 GACAGTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1730 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1258 GACAGTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1271 TGGCCA TGG^CCA
BanII 1017 GAGCCC G[GA]GC[CT]^C
BbvCI 822 GCTGAGG CCTCAGC(-5/-2)
BciVI 4206 GTATCC GTATCC(6/5)
BglI 2322 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4821 TACGTA [CT]AC^GT[GA]
BsaWI 1211 ACCGGA [AT]^CCGG[AT]
BsmBI 1451 CGTCTC CGTCTC(1/5)
BspLU11I 2153 ACATGT A^CATGT
BspMI 428 ACCTGC ACCTGC(4/8)
BssSI 2070 CTCGTG CACGAG(-5/-1)
Bst1107I 4146 GTATAC GTA^TAC
BstAPI 1310 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2660 CCAGCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4146 GTATAC GTA^TAC
CfoI 4916 GCGC GCG^C
EaeI 1271 TGGCCA [CT]^GGCC[GA]
Eam1105I 1258 GACAGTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1634 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4958 GAATTC G^AATTC
EcoRV 1371 GATATC GAT^ATC
HapII 1212 CCGG C^CGG
HhaI 4916 GCGC GCG^C
HinP1I 4916 GCGC G^CGC
HindIII 2792 AAGCTT A^AGCTT
HpaII 1212 CCGG C^CGG
MluNI 1271 TGGCCA TGG^CCA
MscI 1271 TGGCCA TGG^CCA
MspI 1212 CCGG C^CGG
NdeI 517 CATATG CA^TATG
PaeR7I 1719 CTCGAG C^TCGAG
PshAI 1665 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 198 AACGTT AA^CGTT
SexAI 281 ACCAGGT A^CC[AT]GGT
SfcI 4693 CTATAG C^T[GA][CT]AG
SnaBI 4821 TACGTA TAC^GTA
SphI 1747 GCATGC GCATG^C
XhoI 1719 CTCGAG C^TCGAG
XmnI 1730 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AciI 63 CCGC CCGC(-3/-1)
1563 CCGC
AflII 2115 CTTAAG C^TTAAG
2342 CTTAAG
AflIII 2153 ACATGT A^C[GA][CT]GT
3557 ACGTGT
AlwNI 1392 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1571 CAGAGCCTG
AocI 1824 CCTGAGG CC^T[ACGT]AGG
3063 CCTGAGG
AseI 2729 ATTAAT AT^TAAT
4970 ATTAAT
AsnI 2729 ATTAAT AT^TAAT
4970 ATTAAT
AspHI 1878 GAGCAC G[AT]GC[AT]^C
3813 GTGCTC
AvaI 928 CTCGGG C^[CT]CG[GA]G
1719 CTCGAG
AvrII 3610 CCTAGG C^CTAGG
3672 CCTAGG
BbsI 93 GAAGAC GAAGAC(2/6)
2477 GTCTTC
BclI 1870 TGATCA T^GATCA
4321 TGATCA
BfrI 2115 CTTAAG C^TTAAG
2342 CTTAAG
BglII 4841 AGATCT A^GATCT
4985 AGATCT
BlnI 3610 CCTAGG C^CTAGG
3672 CCTAGG
BlpI 2313 GCTCAGC GC^T[ACGT]AGC
2794 GCTTAGC
Bpu1102I 2313 GCTCAGC GC^T[ACGT]AGC
2794 GCTTAGC
BpuAI 93 GAAGAC GAAGAC(2/6)
2477 GTCTTC
BsaI 760 GAGACC GGTCTC(1/5)
3459 GAGACC
BsiHKAI 1878 GAGCAC G[AT]GC[AT]^C
3813 GTGCTC
BsoBI 928 CTCGGG C^[CT]CG[GA]G
1719 CTCGAG
BsrDI 1175 CATTGC GCAATG(2/0)
1660 GCAATG
Bsu36I 1824 CCTGAGG CC^T[ACGT]AGG
3063 CCTGAGG
CelII 2313 GCTCAGC GC^T[ACGT]AGC
2794 GCTTAGC
DraII 748 AGGTCCT [GA]G^G[ACGT]CC[CT]
1828 AGGACCC
DraIII 615 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4792 CACTATGTG
Eco81I 1824 CCTGAGG CC^T[ACGT]AGG
3063 CCTGAGG
EcoO109I 748 AGGTCCT [GA]G^G[ACGT]CC[CT]
1828 AGGACCC
EcoT22I 1155 ATGCAT ATGCA^T
1749 ATGCAT
FauI 62 CCCGC CCCGC(4/6)
1562 CCCGC
FbaI 1870 TGATCA T^GATCA
4321 TGATCA
MfeI 1275 CAATTG C^AATTG
4287 CAATTG
MslI 1335 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4178 CATTTTTGTG
MspA1I 4594 CAGCTG C[AC]G^C[GT]G
4963 CAGCTG
MunI 1275 CAATTG C^AATTG
4287 CAATTG
NsiI 1155 ATGCAT ATGCA^T
1749 ATGCAT
PacI 165 TTAATTAA TTAAT^TAA
3271 TTAATTAA
PflMI 1274 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1570 CCAGAGCCTGG
PpuMI 748 AGGTCCT [GA]G^G[AT]CC[CT]
1828 AGGACCC
PshBI 2729 ATTAAT AT^TAAT
4970 ATTAAT
PvuII 4594 CAGCTG CAG^CTG
4963 CAGCTG
Van91I 1274 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1570 CCAGAGCCTGG
XbaI 4222 TCTAGA T^CTAGA
4988 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII