##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AvaII AviII AvrII
BalI BbrPI BclI BglI BlnI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstBI Bsu36I
Cfr10I ClaI CpoI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
FbaI FseI FspI KpnI KspI
MluI MluNI MroI MscI NaeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SpeI
Sse8387I SspBI SwaI XcmI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 5152 GGCGCC G[GA]^CG[CT]C
AflII 2062 CTTAAG C^TTAAG
Alw44I 900 GTGCAC G^TGCAC
ApaLI 900 GTGCAC G^TGCAC
AspHI 900 GTGCAC G[AT]GC[AT]^C
BamHI 3951 GGATCC G^GATCC
BbeI 5152 GGCGCC GGCGC^C
BcgI 5052 GCAGGTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2062 CTTAAG C^TTAAG
BsaAI 3872 TACGTA [CT]AC^GT[GA]
BsaBI 1707 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5152 GGCGCC G[GA]^CG[CT]C
BsiHKAI 900 GTGCAC G[AT]GC[AT]^C
Bst1107I 3687 GTATAC GTA^TAC
BstEII 3995 GGTAACC G^GT[ACGT]ACC
BstPI 3995 GGTAACC G^GT[ACGT]ACC
BstZ17I 3687 GTATAC GTA^TAC
DraII 3860 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 3860 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3995 GGTAACC G^GT[ACGT]ACC
EcoRV 4227 GATATC GAT^ATC
EcoT14I 2055 CCATGG C^C[AT][AT]GG
HaeII 5152 GGCGCC [GA]GCGC^[CT]
HgaI 3578 GACGC GACGC(5/10)
Hin1I 5152 GGCGCC G[GA]^CG[CT]C
HpaI 1133 GTTAAC GTT^AAC
KasI 5152 GGCGCC G^GCGCC
MamI 1707 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1192 CAATTG C^AATTG
MspA1I 4035 CAGCTG C[AC]G^C[GT]G
MunI 1192 CAATTG C^AATTG
NarI 5152 GGCGCC GG^CGCC
NcoI 2055 CCATGG C^CATGG
PaeR7I 4476 CTCGAG C^TCGAG
PvuII 4035 CAGCTG CAG^CTG
SexAI 4303 ACCAGGT A^CC[AT]GGT
SfoI 5152 GGCGCC GGC^GCC
SmaI 5204 CCCGGG CCC^GGG
SnaBI 3872 TACGTA TAC^GTA
SphI 4455 GCATGC GCATG^C
StyI 2055 CCATGG C^C[AT][AT]GG
XbaI 358 TCTAGA T^CTAGA
XhoI 4476 CTCGAG C^TCGAG
XmaCI 5204 CCCGGG C^CCGGG
XmaI 5204 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 3687 GTATAC GT^[AC][GT]AC
3737 GTAGAC
AlwNI 86 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1643 CAGAGTCTG
BanI 3888 GGCACC G^G[CT][GA]CC
5152 GGCGCC
BanII 1355 GAGCCC G[GA]GC[CT]^C
4040 GAGCCC
BbsI 5395 GAAGAC GAAGAC(2/6)
3905 GTCTTC
BciVI 2134 GGATAC GTATCC(6/5)
5515 GGATAC
BlpI 1107 GCTTAGC GC^T[ACGT]AGC
4037 GCTGAGC
Bpu1102I 1107 GCTTAGC GC^T[ACGT]AGC
4037 GCTGAGC
BpuAI 5395 GAAGAC GAAGAC(2/6)
3905 GTCTTC
BseRI 826 CTCCTC GAGGAG(10/8)
1159 GAGGAG
BspHI 3796 TCATGA T^CATGA
4791 TCATGA
BspMI 3891 ACCTGC ACCTGC(4/8)
5052 GCAGGT
BsrDI 738 GCAATG GCAATG(2/0)
5384 GCAATG
BstAPI 85 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5384 GCAATGGATGC
BstXI 4332 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5031 CCAGCACTTTGG
CelII 1107 GCTTAGC GC^T[ACGT]AGC
4037 GCTGAGC
DraIII 3556 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5520 CACACAGTG
DrdI 1638 GACTGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3805 GACAGAACTGTC
DsaI 2055 CCATGG C^C[GA][CT]GG
2856 CCGTGG
FauI 59 GCGGG CCCGC(4/6)
3008 GCGGG
HapII 5137 CCGG C^CGG
5205 CCGG
HincII 1133 GTTAAC GT[CT]^[GA]AC
1291 GTCAAC
HindII 1133 GTTAAC GT[CT]^[GA]AC
1291 GTCAAC
HpaII 5137 CCGG C^CGG
5205 CCGG
MspI 5137 CCGG C^CGG
5205 CCGG
NdeI 1487 CATATG CA^TATG
2433 CATATG
PflMI 481 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4939 CCAAAAGTTGG
RcaI 3796 TCATGA T^CATGA
4791 TCATGA
Van91I 481 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4939 CCAAAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspI AvaI BbvCI BbvI
BcnI BfaI BglII BmyI BpmI
BsaI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoNI
EcoRI EcoRII EcoT22I Fnu4HI FokI
HaeIII HhaI HinP1I HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflFI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII XmnI