##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
AscI AseI AsnI AspI BamHI
BciVI BglII BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI HindIII
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1302 GGTACC G^GTACC
AccI 372 GTAGAC GT^[AC][GT]AC
AflII 204 CTTAAG C^TTAAG
AflIII 3072 ACACGT A^C[GA][CT]GT
AhdI 2252 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1302 GGTACC G^GTACC
AspEI 2252 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1749 TGCGCA TGC^GCA
AvrII 2144 CCTAGG C^CTAGG
BbeI 2267 GGCGCC GGCGC^C
BcgI 543 GCACGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 204 CTTAAG C^TTAAG
BlnI 2144 CCTAGG C^CTAGG
BsiEI 1672 CGACCG CG[GA][CT]^CG
BstXI 2855 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3423 GGATCT [GA]^GATC[CT]
DrdI 416 GACGCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2252 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 998 GAATTC G^AATTC
FspI 1749 TGCGCA TGC^GCA
HpaI 4371 GTTAAC GTT^AAC
KasI 2267 GGCGCC G^GCGCC
KpnI 1302 GGTACC GGTAC^C
MflI 3423 GGATCT [GA]^GATC[CT]
NaeI 3527 GCCGGC GCC^GGC
NarI 2267 GGCGCC GG^CGCC
NgoMIV 3527 GCCGGC G^CCGGC
SbfI 1072 CCTGCAGG CCTGCA^GG
SexAI 1638 ACCTGGT A^CC[AT]GGT
SfiI 201 GGCCTTAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2267 GGCGCC GGC^GCC
Sse8387I 1072 CCTGCAGG CCTGCA^GG
SspI 4247 AATATT AAT^ATT
XcmI 3714 CCAAGTGGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3423 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 998 GAATTC [GA]^AATT[CT]
3721 GAATTT
Alw44I 2737 GTGCAC G^TGCAC
4315 GTGCAC
ApaLI 2737 GTGCAC G^TGCAC
4315 GTGCAC
ApoI 998 GAATTC [GA]^AATT[CT]
3721 GAATTT
Asp700I 2083 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3135 GAATCCCTTC
BbrPI 1135 CACGTG CAC^GTG
3757 CACGTG
BbsI 2260 GTCTTC GAAGAC(2/6)
3041 GTCTTC
BclI 2172 TGATCA T^GATCA
2873 TGATCA
BlpI 1207 GCTCAGC GC^T[ACGT]AGC
1513 GCTGAGC
Bpu1102I 1207 GCTCAGC GC^T[ACGT]AGC
1513 GCTGAGC
BpuAI 2260 GTCTTC GAAGAC(2/6)
3041 GTCTTC
BsaWI 618 ACCGGA [AT]^CCGG[AT]
3119 TCCGGT
BspHI 3633 TCATGA T^CATGA
4264 TCATGA
BsrBI 2299 CCGCTC CCGCTC(-3/-3)
479 GAGCGG
BssSI 1142 CACGAG CACGAG(-5/-1)
2351 CTCGTG
BstAPI 59 GCACTAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1863 GCAGGGGCTGC
CelII 1207 GCTCAGC GC^T[ACGT]AGC
1513 GCTGAGC
FbaI 2172 TGATCA T^GATCA
2873 TGATCA
PflMI 1153 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGGCTGTGG
PmaCI 1135 CACGTG CAC^GTG
3757 CACGTG
PmlI 1135 CACGTG CAC^GTG
3757 CACGTG
PshAI 218 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1241 GACCTCTGTC
RcaI 3633 TCATGA T^CATGA
4264 TCATGA
SacI 1145 GAGCTC GAGCT^C
3655 GAGCTC
SmlI 204 CTTAAG C^T[CT][GA]AG
2138 CTTGAG
Van91I 1153 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGGCTGTGG
XmnI 2083 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3135 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmaCI XmaI