##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AspI AviII BbrPI BbvCI
BciVI BclI BfrI BsaBI BseAI
BsiWI BsmI BspDI BspEI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII KpnI
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RsrII SacII SalI
SapI SfuI SnaBI SpeI SspI
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2074 GACGTC GACGT^C
AccII 1778 CGCG CG^CG
AcsI 1018 AAATTC [GA]^AATT[CT]
AflIII 2010 ACGTGT A^C[GA][CT]GT
AocI 1331 CCTCAGG CC^T[ACGT]AGG
ApaI 779 GGGCCC GGGCC^C
ApoI 1018 AAATTC [GA]^AATT[CT]
AvrII 1651 CCTAGG C^CTAGG
BamHI 1206 GGATCC G^GATCC
BbsI 907 GAAGAC GAAGAC(2/6)
BglII 2142 AGATCT A^GATCT
BlnI 1651 CCTAGG C^CTAGG
BpuAI 907 GAAGAC GAAGAC(2/6)
BsaAI 1856 TACGTG [CT]AC^GT[GA]
BsaWI 835 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1599 TGTACA T^GTACA
BspHI 18 TCATGA T^CATGA
BsrGI 1599 TGTACA T^GTACA
BstUI 1778 CGCG CG^CG
Bsu36I 1331 CCTCAGG CC^T[ACGT]AGG
DraI 455 TTTAAA TTT^AAA
EagI 2754 CGGCCG C^GGCCG
EclXI 2754 CGGCCG C^GGCCG
Eco52I 2754 CGGCCG C^GGCCG
Eco81I 1331 CCTCAGG CC^T[ACGT]AGG
HincII 510 GTTAAC GT[CT]^[GA]AC
HindII 510 GTTAAC GT[CT]^[GA]AC
HpaI 510 GTTAAC GTT^AAC
MslI 19 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1778 CGCG CG^CG
NruI 1777 TCGCGA TCG^CGA
PpuMI 2019 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 1295 CGATCG CGAT^CG
RcaI 18 TCATGA T^CATGA
SacI 2504 GAGCTC GAGCT^C
SbfI 2644 CCTGCAGG CCTGCA^GG
ScaI 2685 AGTACT AGT^ACT
SexAI 1605 ACCAGGT A^CC[AT]GGT
SgrAI 2232 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 821 CCCGGG CCC^GGG
SphI 1983 GCATGC GCATG^C
Sse8387I 2644 CCTGCAGG CCTGCA^GG
SspBI 1599 TGTACA T^GTACA
XbaI 486 TCTAGA T^CTAGA
XmaCI 821 CCCGGG C^CCGGG
XmaI 821 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 419 GTCTAC GT^[AC][GT]AC
1511 GTAGAC
Asp700I 1480 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2317 GAAGCTGTTC
BbeI 1196 GGCGCC GGCGC^C
2762 GGCGCC
BcgI 1450 CGACCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2551 CGAGCCCGCTGC
BsaI 2051 GAGACC GGTCTC(1/5)
877 GGTCTC
BsgI 1808 GTGCAG GTGCAG(16/14)
1943 GTGCAG
BsiEI 1295 CGATCG CG[GA][CT]^CG
2754 CGGCCG
BsrDI 1276 CATTGC GCAATG(2/0)
1641 GCAATG
BssSI 1988 CACGAG CACGAG(-5/-1)
2477 CACGAG
BstEII 573 GGTGACC G^GT[ACGT]ACC
1787 GGTGACC
BstPI 573 GGTGACC G^GT[ACGT]ACC
1787 GGTGACC
DraIII 1802 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2417 CACAAGGTG
DrdI 916 GACGAACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2053 GACCTTCGGGTC
EcoO65I 573 GGTGACC G^GT[ACGT]ACC
1787 GGTGACC
KasI 1196 GGCGCC G^GCGCC
2762 GGCGCC
NaeI 768 GCCGGC GCC^GGC
1898 GCCGGC
NarI 1196 GGCGCC GG^CGCC
2762 GGCGCC
NgoMIV 768 GCCGGC G^CCGGC
1898 GCCGGC
PflMI 545 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2416 CCACAAGGTGG
PvuII 1257 CAGCTG CAG^CTG
2285 CAGCTG
SfiI 746 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2533 GGCCGAGGAGGCC
SfoI 1196 GGCGCC GGC^GCC
2762 GGCGCC
SmlI 150 CTTGAG C^T[CT][GA]AG
2387 CTCAAG
TfiI 143 GAATC G^A[AT]TC
675 GAATC
Van91I 545 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2416 CCACAAGGTGG
XcmI 2081 CCACCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2277 CCACCCTGCAGCTGG
XmnI 1480 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2317 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcyI AfaI AluI Alw44I
AlwI AlwNI Aor51HI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII