##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BcgI BglI BlnI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I FauI FseI FspI HaeII
Hin1I HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflII 2363 CTTAAG C^TTAAG
Alw44I 3357 GTGCAC G^TGCAC
ApaI 2004 GGGCCC GGGCC^C
ApaLI 3357 GTGCAC G^TGCAC
AspHI 3357 GTGCAC G[AT]GC[AT]^C
AvaI 2771 CCCGGG C^[CT]CG[GA]G
AvaII 530 GGTCC G^G[AT]CC
BanI 2098 GGCACC G^G[CT][GA]CC
BbvCI 2747 GCTGAGG CCTCAGC(-5/-2)
BciVI 3766 GGATAC GTATCC(6/5)
BclI 1287 TGATCA T^GATCA
BfrI 2363 CTTAAG C^TTAAG
BlpI 2897 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2897 GCTAAGC GC^T[ACGT]AGC
BsaI 52 GAGACC GGTCTC(1/5)
BsaWI 409 ACCGGA [AT]^CCGG[AT]
BsgI 1797 CTGCAC GTGCAG(16/14)
BsiHKAI 3357 GTGCAC G[AT]GC[AT]^C
BsoBI 2771 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1901 TGTACA T^GTACA
BspDI 509 ATCGAT AT^CGAT
BspLU11I 1617 ACATGT A^CATGT
BsrGI 1901 TGTACA T^GTACA
BssSI 562 CACGAG CACGAG(-5/-1)
BstAPI 2778 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2586 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2897 GCTAAGC GC^T[ACGT]AGC
CfoI 456 GCGC GCG^C
ClaI 509 ATCGAT AT^CGAT
DraIII 572 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1686 GATATC GAT^ATC
EcoT22I 3795 ATGCAT ATGCA^T
FbaI 1287 TGATCA T^GATCA
HgaI 1103 GACGC GACGC(5/10)
HhaI 456 GCGC GCG^C
HinP1I 456 GCGC G^CGC
NsiI 3795 ATGCAT ATGCA^T
PpuMI 529 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 868 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1100 GTCGAC G^TCGAC
ScaI 3614 AGTACT AGT^ACT
SmaI 2771 CCCGGG CCC^GGG
SmlI 2363 CTTAAG C^T[CT][GA]AG
SspBI 1901 TGTACA T^GTACA
XcmI 2693 CCAAAAATTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2771 CCCGGG C^CCGGG
XmaI 2771 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1100 GTCGAC GT^[AC][GT]AC
1542 GTAGAC
AflIII 1503 ACGTGT A^C[GA][CT]GT
1617 ACATGT
AlwNI 2217 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
2779 CAGAGACTG
BamHI 905 GGATCC G^GATCC
3503 GGATCC
BcnI 2771 CCCGG CC^[GC]GG
2772 CCGGG
BglII 1585 AGATCT A^GATCT
3310 AGATCT
BsaAI 1502 TACGTG [CT]AC^GT[GA]
3787 TACGTG
BspMI 1280 ACCTGC ACCTGC(4/8)
1986 GCAGGT
HapII 410 CCGG C^CGG
2772 CCGG
HindIII 1007 AAGCTT A^AGCTT
2900 AAGCTT
HpaII 410 CCGG C^CGG
2772 CCGG
MspI 410 CCGG C^CGG
2772 CCGG
NciI 2771 CCCGG CC^[GC]GG
2772 CCGGG
SpeI 1599 ACTAGT A^CTAGT
2240 ACTAGT
SspI 103 AATATT AAT^ATT
3297 AATATT
StuI 37 AGGCCT AGG^CCT
362 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI