##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AccI AclI AcsI AflII AgeI
Aor51HI ApoI AscI Asp700I AviII
AvrII BamHI BbvCI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsiWI BsmI BspDI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DrdI Eco47III EcoRI EcoT22I FbaI
FspI HindIII HpaI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2658 GGTACC G^GTACC
AccIII 1359 TCCGGA T^CCGGA
AocI 1372 CCTCAGG CC^T[ACGT]AGG
AseI 1678 ATTAAT AT^TAAT
AsnI 1678 ATTAAT AT^TAAT
Asp718I 2658 GGTACC G^GTACC
AspI 2197 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfaI 1806 CTAG C^TAG
BsaBI 538 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1359 TCCGGA T^CCGGA
BsmBI 2168 GAGACG CGTCTC(1/5)
Bsp1407I 315 TGTACA T^GTACA
BspEI 1359 TCCGGA T^CCGGA
BspLU11I 219 ACATGT A^CATGT
BsrDI 321 GCAATG GCAATG(2/0)
BsrGI 315 TGTACA T^GTACA
BssHII 103 GCGCGC G^CGCGC
BssSI 679 CACGAG CACGAG(-5/-1)
BstEII 1641 GGTCACC G^GT[ACGT]ACC
BstPI 1641 GGTCACC G^GT[ACGT]ACC
BstXI 1084 CCAGGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 342 AGATCC [GA]^GATC[CT]
Bsu36I 1372 CCTCAGG CC^T[ACGT]AGG
EarI 1728 GAAGAG CTCTTC(1/4)
Eco81I 1372 CCTCAGG CC^T[ACGT]AGG
EcoNI 511 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1641 GGTCACC G^GT[ACGT]ACC
EcoRV 2875 GATATC GAT^ATC
FseI 1153 GGCCGGCC GGCCGG^CC
KpnI 2658 GGTACC GGTAC^C
Ksp632I 1728 GAAGAG CTCTTC(1/4)
MaeI 1806 CTAG C^TAG
MamI 538 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 342 AGATCC [GA]^GATC[CT]
MroI 1359 TCCGGA T^CCGGA
NspI 219 ACATGT [GA]CATG^[CT]
PflFI 2197 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1512 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1678 ATTAAT AT^TAAT
SmlI 1406 CTCAAG C^T[CT][GA]AG
SspBI 315 TGTACA T^GTACA
StuI 297 AGGCCT AGG^CCT
TfiI 1832 GAATC G^A[AT]TC
Tth111I 2197 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1805 TCTAGA T^CTAGA
XhoII 342 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 640 GACGTC GACGT^C
2668 GACGTC
AhdI 560 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
854 GACACAGGGTC
Alw44I 1175 GTGCAC G^TGCAC
1838 GTGCAC
ApaLI 1175 GTGCAC G^TGCAC
1838 GTGCAC
AspEI 560 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
854 GACACAGGGTC
BalI 1845 TGGCCA TGG^CCA
2235 TGGCCA
BbsI 1313 GTCTTC GAAGAC(2/6)
1915 GTCTTC
BpmI 1657 CTGGAG CTGGAG(16/14)
2743 CTGGAG
BpuAI 1313 GTCTTC GAAGAC(2/6)
1915 GTCTTC
BspHI 816 TCATGA T^CATGA
1672 TCATGA
BsrFI 1032 GCCGGC [GA]^CCGG[CT]
1154 GCCGGC
Cfr10I 1032 GCCGGC [GA]^CCGG[CT]
1154 GCCGGC
Eam1105I 560 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
854 GACACAGGGTC
HincII 328 GTTGAC GT[CT]^[GA]AC
2893 GTTGAC
HindII 328 GTTGAC GT[CT]^[GA]AC
2893 GTTGAC
MaeIII 1642 GTCAC ^GT[ACGT]AC
1873 GTGAC
MluNI 1845 TGGCCA TGG^CCA
2235 TGGCCA
MscI 1845 TGGCCA TGG^CCA
2235 TGGCCA
MseI 1679 TTAA T^TAA
2912 TTAA
NaeI 1032 GCCGGC GCC^GGC
1154 GCCGGC
NgoMIV 1032 GCCGGC G^CCGGC
1154 GCCGGC
PflMI 216 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2502 CCATTGTGTGG
RcaI 816 TCATGA T^CATGA
1672 TCATGA
Tru9I 1679 TTAA T^TAA
2912 TTAA
Tsp45I 1642 GTCAC ^GT[GC]AC
1873 GTGAC
Tsp509I 1677 AATT ^AATT
1681 AATT
Van91I 216 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2502 CCATTGTGTGG
XcmI 2238 CCATTTCTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2440 CCAGGAGGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbrPI BbvI BcgI BcnI BglI
BmyI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI StyI TaqI TseI TspRI
TthHB8I XmaCI XmaI