##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AcyI AgeI Alw44I
AocI Aor51HI ApaLI AscI AviII
AvrII BamHI BbeI BbrPI BbvCI
BglI BlnI BsaAI BsaHI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BssSI BstBI Bsu36I ClaI CpoI
Eco47III Eco81I FseI FspI HaeII
Hin1I KasI KspI MluI MroI
NarI NheI NotI NruI NspV
PinAI PmaCI PmeI PmlI PshAI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 5111 GGTACC G^GTACC
ApaI 1837 GGGCCC GGGCC^C
Asp700I 3770 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5111 GGTACC G^GTACC
BcgI 1233 GCAGGCATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1522 AGATCT A^GATCT
BlpI 5781 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 5781 GCTTAGC GC^T[ACGT]AGC
BsaBI 635 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 16 ACCGGA [AT]^CCGG[AT]
BsiEI 1596 CGGCCG CG[GA][CT]^CG
BsrBI 1493 GAGCGG CCGCTC(-3/-3)
BssHII 1246 GCGCGC G^CGCGC
Bst1107I 4181 GTATAC GTA^TAC
BstEII 6512 GGTGACC G^GT[ACGT]ACC
BstPI 6512 GGTGACC G^GT[ACGT]ACC
BstZ17I 4181 GTATAC GTA^TAC
CelII 5781 GCTTAGC GC^T[ACGT]AGC
DraIII 2660 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1596 CGGCCG C^GGCCG
EclXI 1596 CGGCCG C^GGCCG
Eco52I 1596 CGGCCG C^GGCCG
EcoNI 5955 CCTTCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 6512 GGTGACC G^GT[ACGT]ACC
EcoT22I 4151 ATGCAT ATGCA^T
FauI 1481 GCGGG CCCGC(4/6)
KpnI 5111 GGTACC GGTAC^C
MamI 635 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5006 GCCGGC GCC^GGC
NcoI 6553 CCATGG C^CATGG
NdeI 3365 CATATG CA^TATG
NgoMIV 5006 GCCGGC G^CCGGC
NsiI 4151 ATGCAT ATGCA^T
PaeR7I 1047 CTCGAG C^TCGAG
SapI 1073 GAAGAGC GCTCTTC(1/4)
SmaI 1540 CCCGGG CCC^GGG
XbaI 7045 TCTAGA T^CTAGA
XhoI 1047 CTCGAG C^TCGAG
XmaCI 1540 CCCGGG C^CCGGG
XmaI 1540 CCCGGG C^CCGGG
XmnI 3770 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AclI 2217 AACGTT AA^CGTT
3566 AACGTT
AflII 3410 CTTAAG C^TTAAG
4616 CTTAAG
AhdI 508 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2393 GACACTTGGTC
AspEI 508 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2393 GACACTTGGTC
AspI 1470 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5652 GACATAGTC
BciVI 640 GTATCC GTATCC(6/5)
2381 GTATCC
BclI 2606 TGATCA T^GATCA
6039 TGATCA
BfrI 3410 CTTAAG C^TTAAG
4616 CTTAAG
Bsp1407I 1585 TGTACA T^GTACA
2231 TGTACA
BspLU11I 1582 ACATGT A^CATGT
2895 ACATGT
BsrDI 182 CATTGC GCAATG(2/0)
259 GCAATG
BsrFI 1594 ACCGGC [GA]^CCGG[CT]
5006 GCCGGC
BsrGI 1585 TGTACA T^GTACA
2231 TGTACA
Cfr10I 1594 ACCGGC [GA]^CCGG[CT]
5006 GCCGGC
DrdI 1923 GACCTCCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5469 GACAGAAGGGTC
DsaI 2428 CCACGG C^C[GA][CT]GG
6553 CCATGG
Eam1105I 508 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2393 GACACTTGGTC
EcoRI 4835 GAATTC G^AATTC
5309 GAATTC
EcoRV 3556 GATATC GAT^ATC
5770 GATATC
FbaI 2606 TGATCA T^GATCA
6039 TGATCA
HindIII 5041 AAGCTT A^AGCTT
5602 AAGCTT
HpaI 756 GTTAAC GTT^AAC
5289 GTTAAC
MfeI 511 CAATTG C^AATTG
5090 CAATTG
MunI 511 CAATTG C^AATTG
5090 CAATTG
PacI 3323 TTAATTAA TTAAT^TAA
3745 TTAATTAA
PflFI 1470 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5652 GACATAGTC
PflMI 584 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2919 CCAATACTTGG
Psp1406I 2217 AACGTT AA^CGTT
3566 AACGTT
SexAI 1914 ACCAGGT A^CC[AT]GGT
2952 ACCAGGT
SpeI 3353 ACTAGT A^CTAGT
3464 ACTAGT
SphI 1397 GCATGC GCATG^C
5130 GCATGC
SspBI 1585 TGTACA T^GTACA
2231 TGTACA
StuI 171 AGGCCT AGG^CCT
1124 AGGCCT
Tth111I 1470 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5652 GACATAGTC
Van91I 584 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2919 CCAATACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII