##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflIII ApoI AscI Asp700I Asp718I
AvrII BamHI BbrPI BclI BglII
BlnI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1270 GACGTC GACGT^C
AflII 3969 CTTAAG C^TTAAG
AgeI 3148 ACCGGT A^CCGGT
AhdI 1667 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3385 GTGCAC G^TGCAC
ApaLI 3385 GTGCAC G^TGCAC
AseI 3867 ATTAAT AT^TAAT
AsnI 3867 ATTAAT AT^TAAT
AspEI 1667 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 797 TGCGCA TGC^GCA
BbsI 3954 GAAGAC GAAGAC(2/6)
BcgI 382 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1870 GTATCC GTATCC(6/5)
BfrI 3969 CTTAAG C^TTAAG
BlpI 1369 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1369 GCTCAGC GC^T[ACGT]AGC
BpuAI 3954 GAAGAC GAAGAC(2/6)
BsaWI 3148 ACCGGT [AT]^CCGG[AT]
BsmI 1526 GAATGC GAATGC(1/-1)
BspMI 1928 ACCTGC ACCTGC(4/8)
BssSI 1616 CACGAG CACGAG(-5/-1)
CelII 1369 GCTCAGC GC^T[ACGT]AGC
DraIII 2690 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1667 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 797 TGCGCA TGC^GCA
NcoI 804 CCATGG C^CATGG
PflMI 711 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3148 ACCGGT A^CCGGT
PshBI 3867 ATTAAT AT^TAAT
SacI 1289 GAGCTC GAGCT^C
ScaI 2600 AGTACT AGT^ACT
Van91I 711 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 197 CCTGAGG CC^T[ACGT]AGG
2891 CCTCAGG
Aor51HI 730 AGCGCT AGC^GCT
1721 AGCGCT
AspI 3465 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3768 GACTCCGTC
BbeI 188 GGCGCC GGCGC^C
2847 GGCGCC
BsrDI 2340 GCAATG GCAATG(2/0)
2558 GCAATG
BstYI 2461 AGATCC [GA]^GATC[CT]
3877 GGATCT
Bsu36I 197 CCTGAGG CC^T[ACGT]AGG
2891 CCTCAGG
EarI 3348 CTCTTC CTCTTC(1/4)
1979 GAAGAG
Eco47III 730 AGCGCT AGC^GCT
1721 AGCGCT
Eco81I 197 CCTGAGG CC^T[ACGT]AGG
2891 CCTCAGG
KasI 188 GGCGCC G^GCGCC
2847 GGCGCC
Ksp632I 3348 CTCTTC CTCTTC(1/4)
1979 GAAGAG
MflI 2461 AGATCC [GA]^GATC[CT]
3877 GGATCT
MslI 2832 CACAAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3408 CACGTAAGTG
NarI 188 GGCGCC GG^CGCC
2847 GGCGCC
PaeR7I 1213 CTCGAG C^TCGAG
2429 CTCGAG
PflFI 3465 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3768 GACTCCGTC
PvuII 2283 CAGCTG CAG^CTG
2316 CAGCTG
SfoI 188 GGCGCC GGC^GCC
2847 GGCGCC
TfiI 383 GATTC G^A[AT]TC
2533 GATTC
Tth111I 3465 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3768 GACTCCGTC
XhoI 1213 CTCGAG C^TCGAG
2429 CTCGAG
XhoII 2461 AGATCC [GA]^GATC[CT]
3877 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmaI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI