##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaI
BseAI BsiEI BsoBI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FauI FseI FspI HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI PacI PaeR7I PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I StuI SwaI XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 1396 GTAGAC GT^[AC][GT]AC
AciI 306 GCGG CCGC(-3/-1)
AcyI 1354 GACGCC G[GA]^CG[CT]C
AflIII 803 ACATGT A^C[GA][CT]GT
AgeI 2657 ACCGGT A^CCGGT
AocI 2703 CCTGAGG CC^T[ACGT]AGG
AspI 919 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2513 TGGCCA TGG^CCA
BbsI 2653 GAAGAC GAAGAC(2/6)
BbvCI 2032 CCTCAGC CCTCAGC(-5/-2)
BcgI 2283 CGAAGTGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3248 AGATCT A^GATCT
BpuAI 2653 GAAGAC GAAGAC(2/6)
BsaBI 1705 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1354 GACGCC G[GA]^CG[CT]C
BsaWI 2657 ACCGGT [AT]^CCGG[AT]
BsgI 2434 GTGCAG GTGCAG(16/14)
BsiWI 879 CGTACG C^GTACG
BsmBI 2526 GAGACG CGTCTC(1/5)
BsmI 783 GCATTC GAATGC(1/-1)
BspLU11I 803 ACATGT A^CATGT
BspMI 2023 ACCTGC ACCTGC(4/8)
BsrFI 2657 ACCGGT [GA]^CCGG[CT]
BstAPI 100 GCACGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1631 TTCGAA TT^CGAA
BstXI 1502 CCACAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2703 CCTGAGG CC^T[ACGT]AGG
CfoI 863 GCGC GCG^C
Cfr10I 2657 ACCGGT [GA]^CCGG[CT]
DsaI 2073 CCATGG C^C[GA][CT]GG
EaeI 2513 TGGCCA [CT]^GGCC[GA]
Eco81I 2703 CCTGAGG CC^T[ACGT]AGG
EcoNI 289 CCTAACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 862 GGCGCT [GA]GCGC^[CT]
HhaI 863 GCGC GCG^C
Hin1I 1354 GACGCC G[GA]^CG[CT]C
HinP1I 863 GCGC G^CGC
HincII 1431 GTCAAC GT[CT]^[GA]AC
HindII 1431 GTCAAC GT[CT]^[GA]AC
HindIII 258 AAGCTT A^AGCTT
MaeII 192 ACGT A^CGT
MamI 1705 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2513 TGGCCA TGG^CCA
MscI 2513 TGGCCA TGG^CCA
MspA1I 470 CAGCTG C[AC]G^C[GT]G
NcoI 2073 CCATGG C^CATGG
NspV 1631 TTCGAA TT^CGAA
PflFI 919 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2657 ACCGGT A^CCGGT
PpuMI 2323 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 470 CAGCTG CAG^CTG
SacI 70 GAGCTC GAGCT^C
SapI 1777 GAAGAGC GCTCTTC(1/4)
ScaI 4059 AGTACT AGT^ACT
SfuI 1631 TTCGAA TT^CGAA
SmlI 4055 CTTGAG C^T[CT][GA]AG
SpeI 3481 ACTAGT A^CTAGT
Tth111I 919 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AseI 13 ATTAAT AT^TAAT
3644 ATTAAT
AsnI 13 ATTAAT AT^TAAT
3644 ATTAAT
AspHI 70 GAGCTC G[AT]GC[AT]^C
3740 GTGCTC
BseRI 153 CTCCTC GAGGAG(10/8)
2222 GAGGAG
BsiHKAI 70 GAGCTC G[AT]GC[AT]^C
3740 GTGCTC
DraII 2323 AGGTCCT [GA]G^G[ACGT]CC[CT]
3755 AGGCCCT
EcoO109I 2323 AGGTCCT [GA]G^G[ACGT]CC[CT]
3755 AGGCCCT
EcoRI 379 GAATTC G^AATTC
526 GAATTC
HgaI 200 GACGC GACGC(5/10)
1354 GACGC
NdeI 2807 CATATG CA^TATG
3399 CATATG
PflMI 562 CCACGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1177 CCACTCCTTGG
PshBI 13 ATTAAT AT^TAAT
3644 ATTAAT
PstI 701 CTGCAG CTGCA^G
3146 CTGCAG
Van91I 562 CCACGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1177 CCACTCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI Asp700I AvaII BanII BbvI
BclI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspI MunI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI