##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AocI Aor51HI AscI Asp718I AvrII
BbvCI BciVI BfrI BglII BlnI
BsaWI BseAI BsiWI BspEI BsrDI
BstBI BstEII BstPI Bsu36I CpoI
DrdI Eco47III Eco81I EcoO65I EcoRV
FseI KpnI MfeI MluI MroI
MunI NaeI NgoMIV NheI NotI
NruI NspV PaeR7I PinAI PmeI
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AhdI 3139 GACAACTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 174 GGGCCC GGGCC^C
AseI 3131 ATTAAT AT^TAAT
AsnI 3131 ATTAAT AT^TAAT
Asp700I 4900 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3139 GACAACTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1060 CCCGGG C^[CT]CG[GA]G
AviII 546 TGCGCA TGC^GCA
BclI 2501 TGATCA T^GATCA
BlpI 5037 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5037 GCTGAGC GC^T[ACGT]AGC
BsaBI 1711 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 817 CGGCCG CG[GA][CT]^CG
BsmBI 76 CGTCTC CGTCTC(1/5)
BsoBI 1060 CCCGGG C^[CT]CG[GA]G
BspDI 5250 ATCGAT AT^CGAT
BspHI 4706 TCATGA T^CATGA
BssSI 1498 CTCGTG CACGAG(-5/-1)
Bst1107I 2038 GTATAC GTA^TAC
BstZ17I 2038 GTATAC GTA^TAC
CelII 5037 GCTGAGC GC^T[ACGT]AGC
ClaI 5250 ATCGAT AT^CGAT
DraIII 231 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 817 CGGCCG C^GGCCG
Eam1105I 3139 GACAACTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 817 CGGCCG C^GGCCG
Eco52I 817 CGGCCG C^GGCCG
EcoT22I 2825 ATGCAT ATGCA^T
FbaI 2501 TGATCA T^GATCA
FspI 546 TGCGCA TGC^GCA
HpaI 1741 GTTAAC GTT^AAC
MamI 1711 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2825 ATGCAT ATGCA^T
PacI 5536 TTAATTAA TTAAT^TAA
PshAI 3578 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3131 ATTAAT AT^TAAT
RcaI 4706 TCATGA T^CATGA
SacI 1186 GAGCTC GAGCT^C
SmaI 1060 CCCGGG CCC^GGG
SpeI 3143 ACTAGT A^CTAGT
SphI 5173 GCATGC GCATG^C
StuI 1321 AGGCCT AGG^CCT
XmaCI 1060 CCCGGG C^CCGGG
XmaI 1060 CCCGGG C^CCGGG
XmnI 4900 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AclI 1979 AACGTT AA^CGTT
5111 AACGTT
Alw44I 603 GTGCAC G^TGCAC
5071 GTGCAC
ApaLI 603 GTGCAC G^TGCAC
5071 GTGCAC
AspI 334 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5194 GACTCTGTC
BbeI 526 GGCGCC GGCGC^C
754 GGCGCC
BbrPI 1030 CACGTG CAC^GTG
5361 CACGTG
BcgI 474 GCAGCGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5242 GCACGCCCATCG
BglI 94 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
746 GCCGCGGGGGC
BsaAI 1030 CACGTG [CT]AC^GT[GA]
5361 CACGTG
BseRI 88 CTCCTC GAGGAG(10/8)
431 CTCCTC
Bsp1407I 1839 TGTACA T^GTACA
2261 TGTACA
BspLU11I 3560 ACATGT A^CATGT
5732 ACATGT
BsrFI 316 ACCGGC [GA]^CCGG[CT]
1303 ACCGGC
BsrGI 1839 TGTACA T^GTACA
2261 TGTACA
Cfr10I 316 ACCGGC [GA]^CCGG[CT]
1303 ACCGGC
EcoRI 2308 GAATTC G^AATTC
4900 GAATTC
HincII 1741 GTTAAC GT[CT]^[GA]AC
3991 GTTGAC
HindII 1741 GTTAAC GT[CT]^[GA]AC
3991 GTTGAC
HindIII 4667 AAGCTT A^AGCTT
5962 AAGCTT
KasI 526 GGCGCC G^GCGCC
754 GGCGCC
NarI 526 GGCGCC GG^CGCC
754 GGCGCC
NcoI 489 CCATGG C^CATGG
4498 CCATGG
PflFI 334 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5194 GACTCTGTC
PflMI 1609 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3149 CCAAGCACTGG
PmaCI 1030 CACGTG CAC^GTG
5361 CACGTG
PmlI 1030 CACGTG CAC^GTG
5361 CACGTG
Psp1406I 1979 AACGTT AA^CGTT
5111 AACGTT
SapI 1149 GCTCTTC GCTCTTC(1/4)
1188 GCTCTTC
SfoI 526 GGCGCC GGC^GCC
754 GGCGCC
SmlI 1869 CTTGAG C^T[CT][GA]AG
5865 CTCAAG
SspBI 1839 TGTACA T^GTACA
2261 TGTACA
Tth111I 334 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5194 GACTCTGTC
Van91I 1609 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3149 CCAAGCACTGG
XbaI 3230 TCTAGA T^CTAGA
4133 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrBI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII