##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccII AccIII AclI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII BbeI BbrPI BcgI BciVI
BglI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BssHII BstBI
BstUI CelII ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FseI FspI
HaeII KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PmaCI PmeI
PmlI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 821 GACGTC GACGT^C
AflII 3709 CTTAAG C^TTAAG
AgeI 3071 ACCGGT A^CCGGT
AseI 3412 ATTAAT AT^TAAT
AsnI 3412 ATTAAT AT^TAAT
AspHI 3231 GAGCAC G[AT]GC[AT]^C
AvrII 2360 CCTAGG C^CTAGG
BamHI 954 GGATCC G^GATCC
BanI 3520 GGCACC G^G[CT][GA]CC
BanII 3286 GGGCTC G[GA]GC[CT]^C
BclI 2471 TGATCA T^GATCA
BfrI 3709 CTTAAG C^TTAAG
BlnI 2360 CCTAGG C^CTAGG
BsgI 3665 CTGCAC GTGCAG(16/14)
BsiHKAI 3231 GAGCAC G[AT]GC[AT]^C
BsrBI 2654 CCGCTC CCGCTC(-3/-3)
BsrFI 3071 ACCGGT [GA]^CCGG[CT]
BssSI 815 CACGAG CACGAG(-5/-1)
Bst1107I 2111 GTATAC GTA^TAC
BstZ17I 2111 GTATAC GTA^TAC
CfoI 4460 GCGC GCG^C
Cfr10I 3071 ACCGGT [GA]^CCGG[CT]
DraII 1018 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 538 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1018 GGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 3537 CCCGC CCCGC(4/6)
FbaI 2471 TGATCA T^GATCA
HhaI 4460 GCGC GCG^C
HinP1I 4460 GCGC G^CGC
NdeI 224 CATATG CA^TATG
PacI 3063 TTAATTAA TTAAT^TAA
PinAI 3071 ACCGGT A^CCGGT
PpuMI 1018 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 5106 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3412 ATTAAT AT^TAAT
SalI 863 GTCGAC G^TCGAC
ScaI 3021 AGTACT AGT^ACT
StuI 2363 AGGCCT AGG^CCT
SwaI 3278 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 821 GACGTC G[GA]^CG[CT]C
860 GGCGTC
AflIII 1825 ACATGT A^C[GA][CT]GT
3576 ACATGT
AocI 2978 CCTGAGG CC^T[ACGT]AGG
4722 CCTCAGG
Asp700I 794 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2689 GAATTTTTTC
BalI 3582 TGGCCA TGG^CCA
4269 TGGCCA
BbvCI 3491 CCTCAGC CCTCAGC(-5/-2)
3625 CCTCAGC
BcnI 846 CCGGG CC^[GC]GG
1305 CCGGG
BglII 115 AGATCT A^GATCT
433 AGATCT
BmyI 3231 GAGCAC G[AGT]GC[ACT]^C
3286 GGGCTC
BpmI 2710 CTGGAG CTGGAG(16/14)
4151 CTGGAG
BsaHI 821 GACGTC G[GA]^CG[CT]C
860 GGCGTC
BseRI 1145 CTCCTC GAGGAG(10/8)
34 GAGGAG
Bsp1286I 3231 GAGCAC G[AGT]GC[ACT]^C
3286 GGGCTC
Bsp1407I 2536 TGTACA T^GTACA
3943 TGTACA
BspLU11I 1825 ACATGT A^CATGT
3576 ACATGT
BsrGI 2536 TGTACA T^GTACA
3943 TGTACA
BstAPI 1074 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4978 GCAATCCATGC
BstEII 2626 GGTAACC G^GT[ACGT]ACC
4471 GGTCACC
BstPI 2626 GGTAACC G^GT[ACGT]ACC
4471 GGTCACC
Bsu36I 2978 CCTGAGG CC^T[ACGT]AGG
4722 CCTCAGG
EarI 162 GAAGAG CTCTTC(1/4)
3949 GAAGAG
Eco81I 2978 CCTGAGG CC^T[ACGT]AGG
4722 CCTCAGG
EcoO65I 2626 GGTAACC G^GT[ACGT]ACC
4471 GGTCACC
EcoT22I 796 ATGCAT ATGCA^T
4985 ATGCAT
HgaI 780 GCGTC GACGC(5/10)
861 GCGTC
Hin1I 821 GACGTC G[GA]^CG[CT]C
860 GGCGTC
HindIII 2379 AAGCTT A^AGCTT
4283 AAGCTT
Ksp632I 162 GAAGAG CTCTTC(1/4)
3949 GAAGAG
MfeI 185 CAATTG C^AATTG
2246 CAATTG
MluNI 3582 TGGCCA TGG^CCA
4269 TGGCCA
MscI 3582 TGGCCA TGG^CCA
4269 TGGCCA
MunI 185 CAATTG C^AATTG
2246 CAATTG
NciI 846 CCGGG CC^[GC]GG
1305 CCGGG
NsiI 796 ATGCAT ATGCA^T
4985 ATGCAT
PflMI 644 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
966 CCAGGAAGTGG
PvuII 1531 CAGCTG CAG^CTG
1641 CAGCTG
SphI 1592 GCATGC GCATG^C
2287 GCATGC
SspBI 2536 TGTACA T^GTACA
3943 TGTACA
Van91I 644 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
966 CCAGGAAGTGG
XmnI 794 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2689 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BbsI BbvI
BfaI BpuAI BsaI BsaJI BsaWI
BsiYI BslI BsmAI BsmFI BsmI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI EaeI EcoNI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII