##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI DraIII DrdI
Eam1105I Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2861 GGTACC G^GTACC
Asp718I 2861 GGTACC G^GTACC
AspI 3157 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 901 TGCGCA TGC^GCA
BalI 3291 TGGCCA TGG^CCA
BbeI 2834 GGCGCC GGCGC^C
BcgI 2572 GCACCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 2212 CGGCCG CG[GA][CT]^CG
BsmBI 749 GAGACG CGTCTC(1/5)
BsmI 104 GAATGC GAATGC(1/-1)
BsrBI 928 GAGCGG CCGCTC(-3/-3)
BssHII 2191 GCGCGC G^CGCGC
BstEII 1984 GGTGACC G^GT[ACGT]ACC
BstPI 1984 GGTGACC G^GT[ACGT]ACC
CpoI 1760 CGGTCCG CG^G[AT]CCG
DraI 3556 TTTAAA TTT^AAA
EagI 2212 CGGCCG C^GGCCG
EclXI 2212 CGGCCG C^GGCCG
Eco52I 2212 CGGCCG C^GGCCG
EcoNI 2050 CCTTCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1984 GGTGACC G^GT[ACGT]ACC
FspI 901 TGCGCA TGC^GCA
KasI 2834 GGCGCC G^GCGCC
KpnI 2861 GGTACC GGTAC^C
MaeII 2172 ACGT A^CGT
MluNI 3291 TGGCCA TGG^CCA
MscI 3291 TGGCCA TGG^CCA
MslI 3298 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2834 GGCGCC GG^CGCC
NcoI 1300 CCATGG C^CATGG
PflFI 3157 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 1760 CGGTCCG CG^G[AT]CCG
SfoI 2834 GGCGCC GGC^GCC
SgrAI 1901 CACCGGCG C[GA]^CCGG[CT]G
SphI 714 GCATGC GCATG^C
Tth111I 3157 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1172 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 2192 CGCG CG^CG
2194 CGCG
AccIII 3231 TCCGGA T^CCGGA
3257 TCCGGA
BglI 2028 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2363 GCCCGGACGGC
BseAI 3231 TCCGGA T^CCGGA
3257 TCCGGA
BsgI 1950 GTGCAG GTGCAG(16/14)
2652 GTGCAG
BspEI 3231 TCCGGA T^CCGGA
3257 TCCGGA
BstUI 2192 CGCG CG^CG
2194 CGCG
MroI 3231 TCCGGA T^CCGGA
3257 TCCGGA
MvnI 2192 CGCG CG^CG
2194 CGCG
NaeI 2214 GCCGGC GCC^GGC
2776 GCCGGC
NgoMIV 2214 GCCGGC G^CCGGC
2776 GCCGGC
NspI 714 GCATGC [GA]CATG^[CT]
1809 ACATGC
PpuMI 979 GGGACCC [GA]G^G[AT]CC[CT]
1662 AGGACCT
TaqI 852 TCGA T^CGA
3147 TCGA
TthHB8I 852 TCGA T^CGA
3147 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII XmaCI XmaI