################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BfrI BglII BlnI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI BstBI BstEII BstPI ClaI CpoI DraI DraIII Eam1105I EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KasI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 2963 GTATAC GT^[AC][GT]AC AccIII 1976 TCCGGA T^CCGGA Aor51HI 2909 AGCGCT AGC^GCT BbsI 1677 GAAGAC GAAGAC(2/6) BclI 2884 TGATCA T^GATCA BlpI 366 GCTGAGC GC^T[ACGT]AGC Bpu1102I 366 GCTGAGC GC^T[ACGT]AGC BpuAI 1677 GAAGAC GAAGAC(2/6) BsaAI 2505 CACGTA [CT]AC^GT[GA] BsaBI 567 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1976 TCCGGA [AT]^CCGG[AT] BseAI 1976 TCCGGA T^CCGGA BsiEI 1876 CGGCCG CG[GA][CT]^CG BsmBI 2097 CGTCTC CGTCTC(1/5) BspEI 1976 TCCGGA T^CCGGA BsrFI 2211 GCCGGC [GA]^CCGG[CT] Bst1107I 2963 GTATAC GTA^TAC BstYI 355 AGATCC [GA]^GATC[CT] BstZ17I 2963 GTATAC GTA^TAC CelII 366 GCTGAGC GC^T[ACGT]AGC Cfr10I 2211 GCCGGC [GA]^CCGG[CT] DrdI 1232 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1876 CGGCCG C^GGCCG EclXI 1876 CGGCCG C^GGCCG Eco47III 2909 AGCGCT AGC^GCT Eco52I 1876 CGGCCG C^GGCCG FbaI 2884 TGATCA T^GATCA HincII 900 GTCAAC GT[CT]^[GA]AC HindII 900 GTCAAC GT[CT]^[GA]AC MamI 567 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 355 AGATCC [GA]^GATC[CT] MroI 1976 TCCGGA T^CCGGA MseI 3035 TTAA T^TAA NaeI 2211 GCCGGC GCC^GGC NgoMIV 2211 GCCGGC G^CCGGC PaeR7I 1921 CTCGAG C^TCGAG PshAI 1817 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2292 GAAGAGC GCTCTTC(1/4) SfiI 1892 GGCCTCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 1891 AGGCCT AGG^CCT Tru9I 3035 TTAA T^TAA XhoI 1921 CTCGAG C^TCGAG XhoII 355 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AatII 1238 GACGTC GACGT^C 2095 GACGTC Acc65I 1619 GGTACC G^GTACC 2626 GGTACC AccII 1309 CGCG CG^CG 2150 CGCG AocI 1497 CCTCAGG CC^T[ACGT]AGG 1903 CCTCAGG Asp700I 439 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1754 GAACCTGTTC Asp718I 1619 GGTACC G^GTACC 2626 GGTACC AspI 2112 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3324 GACCCTGTC BsrDI 2500 CATTGC GCAATG(2/0) 126 GCAATG BstAPI 524 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1547 GCAGGAGGTGC BstUI 1309 CGCG CG^CG 2150 CGCG Bsu36I 1497 CCTCAGG CC^T[ACGT]AGG 1903 CCTCAGG Eco81I 1497 CCTCAGG CC^T[ACGT]AGG 1903 CCTCAGG EcoRI 163 GAATTC G^AATTC 1116 GAATTC HgaI 1637 GACGC GACGC(5/10) 2225 GACGC KpnI 1619 GGTACC GGTAC^C 2626 GGTACC MvnI 1309 CGCG CG^CG 2150 CGCG PflFI 2112 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3324 GACCCTGTC SmaI 1432 CCCGGG CCC^GGG 2340 CCCGGG SphI 1157 GCATGC GCATG^C 1954 GCATGC TaqI 958 TCGA T^CGA 1922 TCGA Tth111I 2112 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3324 GACCCTGTC TthHB8I 958 TCGA T^CGA 1922 TCGA XcmI 838 CCAGGGGCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2044 CCAGCCAGTTCCTGG XmaCI 1432 CCCGGG C^CCGGG 2340 CCCGGG XmaI 1432 CCCGGG C^CCGGG 2340 CCCGGG XmnI 439 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1754 GAACCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI TseI Tsp45I Tsp509I TspRI Van91I