##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AclI AflII AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BfrI BglII
BlnI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BssSI
BstBI BstEII BstPI ClaI CpoI
DraI DraIII Eam1105I EcoO65I EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2963 GTATAC GT^[AC][GT]AC
AccIII 1976 TCCGGA T^CCGGA
Aor51HI 2909 AGCGCT AGC^GCT
BbsI 1677 GAAGAC GAAGAC(2/6)
BclI 2884 TGATCA T^GATCA
BlpI 366 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 366 GCTGAGC GC^T[ACGT]AGC
BpuAI 1677 GAAGAC GAAGAC(2/6)
BsaAI 2505 CACGTA [CT]AC^GT[GA]
BsaBI 567 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1976 TCCGGA [AT]^CCGG[AT]
BseAI 1976 TCCGGA T^CCGGA
BsiEI 1876 CGGCCG CG[GA][CT]^CG
BsmBI 2097 CGTCTC CGTCTC(1/5)
BspEI 1976 TCCGGA T^CCGGA
BsrFI 2211 GCCGGC [GA]^CCGG[CT]
Bst1107I 2963 GTATAC GTA^TAC
BstYI 355 AGATCC [GA]^GATC[CT]
BstZ17I 2963 GTATAC GTA^TAC
CelII 366 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2211 GCCGGC [GA]^CCGG[CT]
DrdI 1232 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1876 CGGCCG C^GGCCG
EclXI 1876 CGGCCG C^GGCCG
Eco47III 2909 AGCGCT AGC^GCT
Eco52I 1876 CGGCCG C^GGCCG
FbaI 2884 TGATCA T^GATCA
HincII 900 GTCAAC GT[CT]^[GA]AC
HindII 900 GTCAAC GT[CT]^[GA]AC
MamI 567 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 355 AGATCC [GA]^GATC[CT]
MroI 1976 TCCGGA T^CCGGA
MseI 3035 TTAA T^TAA
NaeI 2211 GCCGGC GCC^GGC
NgoMIV 2211 GCCGGC G^CCGGC
PaeR7I 1921 CTCGAG C^TCGAG
PshAI 1817 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2292 GAAGAGC GCTCTTC(1/4)
SfiI 1892 GGCCTCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1891 AGGCCT AGG^CCT
Tru9I 3035 TTAA T^TAA
XhoI 1921 CTCGAG C^TCGAG
XhoII 355 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 1238 GACGTC GACGT^C
2095 GACGTC
Acc65I 1619 GGTACC G^GTACC
2626 GGTACC
AccII 1309 CGCG CG^CG
2150 CGCG
AocI 1497 CCTCAGG CC^T[ACGT]AGG
1903 CCTCAGG
Asp700I 439 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1754 GAACCTGTTC
Asp718I 1619 GGTACC G^GTACC
2626 GGTACC
AspI 2112 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3324 GACCCTGTC
BsrDI 2500 CATTGC GCAATG(2/0)
126 GCAATG
BstAPI 524 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1547 GCAGGAGGTGC
BstUI 1309 CGCG CG^CG
2150 CGCG
Bsu36I 1497 CCTCAGG CC^T[ACGT]AGG
1903 CCTCAGG
Eco81I 1497 CCTCAGG CC^T[ACGT]AGG
1903 CCTCAGG
EcoRI 163 GAATTC G^AATTC
1116 GAATTC
HgaI 1637 GACGC GACGC(5/10)
2225 GACGC
KpnI 1619 GGTACC GGTAC^C
2626 GGTACC
MvnI 1309 CGCG CG^CG
2150 CGCG
PflFI 2112 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3324 GACCCTGTC
SmaI 1432 CCCGGG CCC^GGG
2340 CCCGGG
SphI 1157 GCATGC GCATG^C
1954 GCATGC
TaqI 958 TCGA T^CGA
1922 TCGA
Tth111I 2112 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3324 GACCCTGTC
TthHB8I 958 TCGA T^CGA
1922 TCGA
XcmI 838 CCAGGGGCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2044 CCAGCCAGTTCCTGG
XmaCI 1432 CCCGGG C^CCGGG
2340 CCCGGG
XmaI 1432 CCCGGG C^CCGGG
2340 CCCGGG
XmnI 439 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1754 GAACCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI TseI Tsp45I Tsp509I
TspRI Van91I