##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccI AflIII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AspI AviII BamHI BbsI
BciVI BpuAI BsiWI Bsp1407I BspDI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstZ17I ClaI DrdI Eam1105I EcoRI
EcoT22I FspI HincII HindII HpaI
KpnI MfeI MluI MslI MunI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PvuI SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 740 AACGTT AA^CGTT
AflII 2233 CTTAAG C^TTAAG
AocI 290 CCTGAGG CC^T[ACGT]AGG
Aor51HI 390 AGCGCT AGC^GCT
AseI 733 ATTAAT AT^TAAT
AsnI 733 ATTAAT AT^TAAT
BbrPI 2175 CACGTG CAC^GTG
BcgI 2155 CGAATCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1960 TGATCA T^GATCA
BfrI 2233 CTTAAG C^TTAAG
BglII 1195 AGATCT A^GATCT
BlpI 2165 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2165 GCTGAGC GC^T[ACGT]AGC
BsaAI 2175 CACGTG [CT]AC^GT[GA]
BsaBI 1707 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2934 GGTCTC GGTCTC(1/5)
BsmI 1020 GAATGC GAATGC(1/-1)
BspHI 1134 TCATGA T^CATGA
BsrBI 644 GAGCGG CCGCTC(-3/-3)
BstAPI 160 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 778 GGTTACC G^GT[ACGT]ACC
BstPI 778 GGTTACC G^GT[ACGT]ACC
Bsu36I 290 CCTGAGG CC^T[ACGT]AGG
CelII 2165 GCTGAGC GC^T[ACGT]AGC
CpoI 126 CGGTCCG CG^G[AT]CCG
DraIII 1826 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 390 AGCGCT AGC^GCT
Eco81I 290 CCTGAGG CC^T[ACGT]AGG
EcoO65I 778 GGTTACC G^GT[ACGT]ACC
EcoRV 974 GATATC GAT^ATC
FbaI 1960 TGATCA T^GATCA
FseI 319 GGCCGGCC GGCCGG^CC
HindIII 854 AAGCTT A^AGCTT
MamI 1707 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 3552 CATATG CA^TATG
NheI 1111 GCTAGC G^CTAGC
NspI 3159 GCATGC [GA]CATG^[CT]
PaeR7I 1116 CTCGAG C^TCGAG
PmaCI 2175 CACGTG CAC^GTG
PmlI 2175 CACGTG CAC^GTG
PshAI 887 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 733 ATTAAT AT^TAAT
Psp1406I 740 AACGTT AA^CGTT
RcaI 1134 TCATGA T^CATGA
RsrII 126 CGGTCCG CG^G[AT]CCG
SacI 2247 GAGCTC GAGCT^C
SapI 3439 GAAGAGC GCTCTTC(1/4)
SphI 3159 GCATGC GCATG^C
XbaI 3468 TCTAGA T^CTAGA
XcmI 3387 CCAGCCCATAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1116 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccIII 1530 TCCGGA T^CCGGA
2256 TCCGGA
ApaI 258 GGGCCC GGGCC^C
269 GGGCCC
Asp700I 1094 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1193 GAAGATCTTC
AvrII 61 CCTAGG C^CTAGG
889 CCTAGG
BbvCI 2832 CCTCAGC CCTCAGC(-5/-2)
599 GCTGAGG
BlnI 61 CCTAGG C^CTAGG
889 CCTAGG
BsaWI 1530 TCCGGA [AT]^CCGG[AT]
2256 TCCGGA
BseAI 1530 TCCGGA T^CCGGA
2256 TCCGGA
BspEI 1530 TCCGGA T^CCGGA
2256 TCCGGA
BssSI 2951 CTCGTG CACGAG(-5/-1)
3248 CTCGTG
DraI 1156 TTTAAA TTT^AAA
1718 TTTAAA
EcoNI 2229 CCTCCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2644 CCTTGTGTAGG
MroI 1530 TCCGGA T^CCGGA
2256 TCCGGA
NaeI 320 GCCGGC GCC^GGC
2272 GCCGGC
NgoMIV 320 GCCGGC G^CCGGC
2272 GCCGGC
PflMI 769 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1480 CCAGCAGCTGG
ScaI 804 AGTACT AGT^ACT
3518 AGTACT
Van91I 769 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1480 CCAGCAGCTGG
XmnI 1094 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1193 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII