##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AviII BamHI BanII
BbeI BbrPI BfrI BglI BlpI
Bpu1102I BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FauI FseI
FspI HindIII HpaI KasI KspI
MluI MroI NarI NdeI NotI
NruI NspV PacI PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspI Van91I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2784 GGTACC G^GTACC
AccI 1157 GTAGAC GT^[AC][GT]AC
AgeI 2420 ACCGGT A^CCGGT
Alw44I 2521 GTGCAC G^TGCAC
ApaLI 2521 GTGCAC G^TGCAC
Asp700I 3019 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2784 GGTACC G^GTACC
AspI 2973 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1379 CTCGAG C^[CT]CG[GA]G
BbvCI 3104 CCTCAGC CCTCAGC(-5/-2)
BclI 594 TGATCA T^GATCA
BcnI 1316 CCGGG CC^[GC]GG
BglII 1238 AGATCT A^GATCT
BsaAI 2913 CACGTA [CT]AC^GT[GA]
BsaBI 589 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2420 ACCGGT [AT]^CCGG[AT]
BseRI 1275 CTCCTC GAGGAG(10/8)
BsgI 440 GTGCAG GTGCAG(16/14)
BsoBI 1379 CTCGAG C^[CT]CG[GA]G
DrdI 1051 GACCGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 285 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2723 GAATTC G^AATTC
EcoT22I 2393 ATGCAT ATGCA^T
FbaI 594 TGATCA T^GATCA
HaeII 4 AGCGCC [GA]GCGC^[CT]
KpnI 2784 GGTACC GGTAC^C
MamI 589 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1855 CAATTG C^AATTG
MunI 1855 CAATTG C^AATTG
NaeI 1454 GCCGGC GCC^GGC
NciI 1316 CCGGG CC^[GC]GG
NgoMIV 1454 GCCGGC G^CCGGC
NheI 2201 GCTAGC G^CTAGC
NsiI 2393 ATGCAT ATGCA^T
PaeR7I 1379 CTCGAG C^TCGAG
PflFI 2973 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2420 ACCGGT A^CCGGT
SapI 313 GAAGAGC GCTCTTC(1/4)
SgrAI 2419 CACCGGTG C[GA]^CCGG[CT]G
StuI 2211 AGGCCT AGG^CCT
SwaI 791 ATTTAAAT ATTT^AAAT
Tth111I 2973 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1379 CTCGAG C^TCGAG
XmnI 3019 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 17 CGCG CG^CG
60 CGCG
AcyI 1354 GGCGTC G[GA]^CG[CT]C
1778 GGCGTC
AlwI 700 GATCC GGATC(4/5)
1084 GATCC
AspHI 316 GAGCAC G[AT]GC[AT]^C
2521 GTGCAC
AvrII 333 CCTAGG C^CTAGG
858 CCTAGG
BalI 288 TGGCCA TGG^CCA
2089 TGGCCA
BanI 346 GGTGCC G^G[CT][GA]CC
2784 GGTACC
BcgI 432 CGAGAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1810 CGAGAAAACTGC
BciVI 3390 GGATAC GTATCC(6/5)
1805 GTATCC
BlnI 333 CCTAGG C^CTAGG
858 CCTAGG
BsaHI 1354 GGCGTC G[GA]^CG[CT]C
1778 GGCGTC
BsaI 1909 GAGACC GGTCTC(1/5)
3009 GGTCTC
BsiHKAI 316 GAGCAC G[AT]GC[AT]^C
2521 GTGCAC
BsmI 2197 GAATGC GAATGC(1/-1)
158 GCATTC
Bsp1407I 2063 TGTACA T^GTACA
3286 TGTACA
BspMI 1912 ACCTGC ACCTGC(4/8)
883 GCAGGT
BsrGI 2063 TGTACA T^GTACA
3286 TGTACA
BstAPI 883 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2241 GCAGACAATGC
BstUI 17 CGCG CG^CG
60 CGCG
BstXI 2209 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3048 CCAGGAAATTGG
BstYI 699 AGATCC [GA]^GATC[CT]
1238 AGATCT
CfoI 5 GCGC GCG^C
61 GCGC
HhaI 5 GCGC GCG^C
61 GCGC
Hin1I 1354 GGCGTC G[GA]^CG[CT]C
1778 GGCGTC
HinP1I 5 GCGC G^CGC
61 GCGC
HincII 303 GTTGAC GT[CT]^[GA]AC
409 GTTGAC
HindII 303 GTTGAC GT[CT]^[GA]AC
409 GTTGAC
MaeII 1101 ACGT A^CGT
2914 ACGT
MflI 699 AGATCC [GA]^GATC[CT]
1238 AGATCT
MluNI 288 TGGCCA TGG^CCA
2089 TGGCCA
MscI 288 TGGCCA TGG^CCA
2089 TGGCCA
MslI 963 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2419 CACCGGTGTG
MvnI 17 CGCG CG^CG
60 CGCG
NcoI 359 CCATGG C^CATGG
2086 CCATGG
SspBI 2063 TGTACA T^GTACA
3286 TGTACA
TaqI 311 TCGA T^CGA
1380 TCGA
TthHB8I 311 TCGA T^CGA
1380 TCGA
XbaI 1942 TCTAGA T^CTAGA
2893 TCTAGA
XhoII 699 AGATCC [GA]^GATC[CT]
1238 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspLU11I BsrDI BsrFI BsrI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI