##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI BsaBI BseAI BsiWI BspDI
BspEI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoRV EcoT22I HindIII
HpaI MamI MfeI MluI MroI
MunI NdeI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfuI SnaBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2516 GGTACC G^GTACC
AccI 3691 GTCTAC GT^[AC][GT]AC
AflII 6380 CTTAAG C^TTAAG
Alw44I 6141 GTGCAC G^TGCAC
ApaLI 6141 GTGCAC G^TGCAC
Asp718I 2516 GGTACC G^GTACC
AspI 6372 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5115 TGCGCA TGC^GCA
AvrII 3443 CCTAGG C^CTAGG
BamHI 2107 GGATCC G^GATCC
BbeI 3516 GGCGCC GGCGC^C
BciVI 2470 GGATAC GTATCC(6/5)
BclI 1217 TGATCA T^GATCA
BfrI 6380 CTTAAG C^TTAAG
BlnI 3443 CCTAGG C^CTAGG
BsaI 4476 GAGACC GGTCTC(1/5)
BsaWI 1898 TCCGGT [AT]^CCGG[AT]
BsmI 6387 GCATTC GAATGC(1/-1)
BsrDI 6261 GCAATG GCAATG(2/0)
BstEII 1035 GGTCACC G^GT[ACGT]ACC
BstPI 1035 GGTCACC G^GT[ACGT]ACC
EcoO65I 1035 GGTCACC G^GT[ACGT]ACC
EcoRI 1990 GAATTC G^AATTC
FbaI 1217 TGATCA T^GATCA
FseI 5486 GGCCGGCC GGCCGG^CC
FspI 5115 TGCGCA TGC^GCA
HincII 1662 GTTGAC GT[CT]^[GA]AC
HindII 1662 GTTGAC GT[CT]^[GA]AC
KasI 3516 GGCGCC G^GCGCC
KpnI 2516 GGTACC GGTAC^C
NarI 3516 GGCGCC GG^CGCC
NheI 2556 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
PaeR7I 3673 CTCGAG C^TCGAG
PflFI 6372 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfiI 5486 GGCCGGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3516 GGCGCC GGC^GCC
SgrAI 3653 CGCCGGTG C[GA]^CCGG[CT]G
StuI 4024 AGGCCT AGG^CCT
Tth111I 6372 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2075 TCTAGA T^CTAGA
XhoI 3673 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 655 AAATTC [GA]^AATT[CT]
1990 GAATTC
AcyI 3192 GGCGTC G[GA]^CG[CT]C
3516 GGCGCC
ApoI 655 AAATTC [GA]^AATT[CT]
1990 GAATTC
BbrPI 3009 CACGTG CAC^GTG
6014 CACGTG
BcgI 5127 CGACAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3665 GCACCTCCCTCG
BglII 2833 AGATCT A^GATCT
2842 AGATCT
BlpI 1434 GCTCAGC GC^T[ACGT]AGC
4921 GCTGAGC
Bpu1102I 1434 GCTCAGC GC^T[ACGT]AGC
4921 GCTGAGC
BsaAI 3009 CACGTG [CT]AC^GT[GA]
6014 CACGTG
BsaHI 3192 GGCGTC G[GA]^CG[CT]C
3516 GGCGCC
BspHI 1220 TCATGA T^CATGA
1698 TCATGA
BsrBI 83 CCGCTC CCGCTC(-3/-3)
776 GAGCGG
BstAPI 1596 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1647 GCATCCTGTGC
CelII 1434 GCTCAGC GC^T[ACGT]AGC
4921 GCTGAGC
DraIII 1386 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
3942 CACGATGTG
HaeII 3516 GGCGCC [GA]GCGC^[CT]
3629 AGCGCC
Hin1I 3192 GGCGTC G[GA]^CG[CT]C
3516 GGCGCC
KspI 1846 CCGCGG CCGC^GG
2885 CCGCGG
NaeI 142 GCCGGC GCC^GGC
5487 GCCGGC
NgoMIV 142 GCCGGC G^CCGGC
5487 GCCGGC
PmaCI 3009 CACGTG CAC^GTG
6014 CACGTG
PmlI 3009 CACGTG CAC^GTG
6014 CACGTG
RcaI 1220 TCATGA T^CATGA
1698 TCATGA
SacII 1846 CCGCGG CCGC^GG
2885 CCGCGG
SapI 4556 GAAGAGC GCTCTTC(1/4)
1605 GCTCTTC
SexAI 1174 ACCTGGT A^CC[AT]GGT
3988 ACCAGGT
SpeI 4155 ACTAGT A^CTAGT
5195 ACTAGT
TaqI 1237 TCGA T^CGA
3674 TCGA
TthHB8I 1237 TCGA T^CGA
3674 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmaI SmlI SphI Sse8387I SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmaCI XmaI XmnI