################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII Acc65I AclI AgeI AscI AseI AsnI Asp718I AspI BamHI BbrPI BcgI BciVI BclI BglII BsiWI BsmI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI BstBI ClaI CpoI DrdI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MluI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AfaI 474 GTAC GT^AC AflII 3682 CTTAAG C^TTAAG AflIII 15 ACGTGT A^C[GA][CT]GT Alw44I 550 GTGCAC G^TGCAC AocI 4529 CCTGAGG CC^T[ACGT]AGG Aor51HI 413 AGCGCT AGC^GCT ApaLI 550 GTGCAC G^TGCAC AviII 1509 TGCGCA TGC^GCA BbsI 3304 GAAGAC GAAGAC(2/6) BfrI 3682 CTTAAG C^TTAAG BpuAI 3304 GAAGAC GAAGAC(2/6) BsaBI 184 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 1296 TCATGA T^CATGA BsrBI 860 CCGCTC CCGCTC(-3/-3) BsrDI 3769 CATTGC GCAATG(2/0) Bst1107I 1542 GTATAC GTA^TAC BstZ17I 1542 GTATAC GTA^TAC Bsu36I 4529 CCTGAGG CC^T[ACGT]AGG Eco47III 413 AGCGCT AGC^GCT Eco81I 4529 CCTGAGG CC^T[ACGT]AGG FspI 1509 TGCGCA TGC^GCA HincII 4524 GTCGAC GT[CT]^[GA]AC HindII 4524 GTCGAC GT[CT]^[GA]AC KspI 81 CCGCGG CCGC^GG MamI 184 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1289 CAATTG C^AATTG MslI 991 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1289 CAATTG C^AATTG NaeI 71 GCCGGC GCC^GGC NgoMIV 71 GCCGGC G^CCGGC NspI 1468 ACATGC [GA]CATG^[CT] PvuI 1178 CGATCG CGAT^CG RcaI 1296 TCATGA T^CATGA RsaI 474 GTAC GT^AC SacII 81 CCGCGG CCGC^GG SalI 4524 GTCGAC G^TCGAC XbaI 118 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 4402 GAATTC [GA]^AATT[CT] 4410 GAATTC AhdI 784 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1943 GACCCTCGGTC ApoI 4402 GAATTC [GA]^AATT[CT] 4410 GAATTC Asp700I 720 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4244 GAAAGCCTTC AspEI 784 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1943 GACCCTCGGTC AvrII 2491 CCTAGG C^CTAGG 4190 CCTAGG BlnI 2491 CCTAGG C^CTAGG 4190 CCTAGG BlpI 40 GCTGAGC GC^T[ACGT]AGC 2929 GCTCAGC Bpu1102I 40 GCTGAGC GC^T[ACGT]AGC 2929 GCTCAGC BsaAI 547 TACGTG [CT]AC^GT[GA] 2281 TACGTG BsaI 1941 GAGACC GGTCTC(1/5) 2707 GGTCTC BsmBI 3745 CGTCTC CGTCTC(1/5) 745 GAGACG BstAPI 262 GCAATCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2230 GCAGACCCTGC CelII 40 GCTGAGC GC^T[ACGT]AGC 2929 GCTCAGC DraI 3532 TTTAAA TTT^AAA 4688 TTTAAA DraIII 1857 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2242 CACACGGTG EagI 843 CGGCCG C^GGCCG 2029 CGGCCG Eam1105I 784 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1943 GACCCTCGGTC EclXI 843 CGGCCG C^GGCCG 2029 CGGCCG Eco52I 843 CGGCCG C^GGCCG 2029 CGGCCG EcoNI 2414 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3214 CCTCAGTAAGG EcoRI 4402 GAATTC G^AATTC 4410 GAATTC NcoI 990 CCATGG C^CATGG 1955 CCATGG SacI 1933 GAGCTC GAGCT^C 2367 GAGCTC SbfI 2218 CCTGCAGG CCTGCA^GG 3187 CCTGCAGG Sse8387I 2218 CCTGCAGG CCTGCA^GG 3187 CCTGCAGG StuI 2665 AGGCCT AGG^CCT 3634 AGGCCT XmnI 720 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4244 GAAAGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccI AccII AccIII AciI AcyI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI