##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanII BbeI BbvCI BbvI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HhaI
HinP1I HpaI ItaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TseI Tth111I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1760 GTAGAC GT^[AC][GT]AC
AcyI 632 GACGCC G[GA]^CG[CT]C
AflII 168 CTTAAG C^TTAAG
AlwNI 1658 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1636 GAGCAC G[AT]GC[AT]^C
AvaII 2152 GGTCC G^G[AT]CC
BamHI 201 GGATCC G^GATCC
BanI 1999 GGCACC G^G[CT][GA]CC
BbrPI 1748 CACGTG CAC^GTG
BfrI 168 CTTAAG C^TTAAG
BmyI 1636 GAGCAC G[AGT]GC[ACT]^C
BsaAI 1748 CACGTG [CT]AC^GT[GA]
BsaHI 632 GACGCC G[GA]^CG[CT]C
BsaWI 1428 ACCGGA [AT]^CCGG[AT]
BseRI 833 GAGGAG GAGGAG(10/8)
BsiHKAI 1636 GAGCAC G[AT]GC[AT]^C
BsmAI 907 GAGAC GTCTC(1/5)
Bsp1286I 1636 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 2199 TGTACA T^GTACA
BspHI 125 TCATGA T^CATGA
BspMI 1492 GCAGGT ACCTGC(4/8)
BsrGI 2199 TGTACA T^GTACA
BssSI 707 CTCGTG CACGAG(-5/-1)
Cfr13I 2152 GGTCC G^G[ACGT]CC
DraII 2151 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 195 CCATGG C^C[GA][CT]GG
EcoNI 1565 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2151 AGGTCCC [GA]G^G[ACGT]CC[CT]
HaeIII 517 GGCC GG^CC
HapII 1429 CCGG C^CGG
HgaI 632 GACGC GACGC(5/10)
Hin1I 632 GACGCC G[GA]^CG[CT]C
HincII 613 GTTGAC GT[CT]^[GA]AC
HindII 613 GTTGAC GT[CT]^[GA]AC
HpaII 1429 CCGG C^CGG
MspI 1429 CCGG C^CGG
NcoI 195 CCATGG C^CATGG
NheI 276 GCTAGC G^CTAGC
PmaCI 1748 CACGTG CAC^GTG
PmlI 1748 CACGTG CAC^GTG
PpuMI 2151 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 125 TCATGA T^CATGA
Sau96I 2152 GGTCC G^G[ACGT]CC
ScaI 2257 AGTACT AGT^ACT
SexAI 1656 ACCAGGT A^CC[AT]GGT
SmlI 168 CTTAAG C^T[CT][GA]AG
SspBI 2199 TGTACA T^GTACA
StuI 516 AGGCCT AGG^CCT
TaqI 237 TCGA T^CGA
TthHB8I 237 TCGA T^CGA
XbaI 468 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 578 CCGC CCGC(-3/-1)
2279 GCGG
AfaI 2200 GTAC GT^AC
2258 GTAC
AseI 1979 ATTAAT AT^TAAT
2366 ATTAAT
AsnI 1979 ATTAAT AT^TAAT
2366 ATTAAT
BbsI 1134 GTCTTC GAAGAC(2/6)
1203 GTCTTC
BpuAI 1134 GTCTTC GAAGAC(2/6)
1203 GTCTTC
BsgI 1031 GTGCAG GTGCAG(16/14)
2060 GTGCAG
BsiYI 578 CCGCATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1565 CCTTCTGTAGG
BslI 578 CCGCATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1565 CCTTCTGTAGG
BsmFI 164 GTCCC GGGAC(10/14)
2153 GTCCC
BsmI 1224 GCATTC GAATGC(1/-1)
1671 GCATTC
BstAPI 715 GCATTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
922 GCAGAGGATGC
BstYI 201 GGATCC [GA]^GATC[CT]
1455 AGATCC
EcoRV 1831 GATATC GAT^ATC
1993 GATATC
MaeII 689 ACGT A^CGT
1749 ACGT
MflI 201 GGATCC [GA]^GATC[CT]
1455 AGATCC
MslI 637 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
790 CACAGAAATG
PleI 403 GAGTC GAGTC(4/5)
1201 GAGTC
PshBI 1979 ATTAAT AT^TAAT
2366 ATTAAT
RsaI 2200 GTAC GT^AC
2258 GTAC
SapI 349 GAAGAGC GCTCTTC(1/4)
296 GCTCTTC
TspRI 1130 CAGTG CA[GC]TG[ACGT][ACGT]^
1890 CAGTG
XhoII 201 GGATCC [GA]^GATC[CT]
1455 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
BfaI BsaJI BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT14I FokI
HindIII HinfI HphI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI ScrFI SfaNI SfcI StyI
TfiI Tru9I Tsp45I Tsp509I XmnI