##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BfrI BglII BlnI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BstBI BstEII BstPI Bsu36I
ClaI CpoI Eam1105I Eco47III Eco81I
EcoO65I EcoRV EcoT22I FseI HpaI
KpnI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SapI ScaI SfuI SgrAI
SnaBI SpeI SspBI SwaI Tth111I
Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2353 GACGTC GACGT^C
AccI 1352 GTATAC GT^[AC][GT]AC
AflIII 3936 ACACGT A^C[GA][CT]GT
Alw44I 3460 GTGCAC G^TGCAC
ApaLI 3460 GTGCAC G^TGCAC
BamHI 73 GGATCC G^GATCC
BbrPI 4123 CACGTG CAC^GTG
BbvCI 2190 GCTGAGG CCTCAGC(-5/-2)
BsaWI 140 TCCGGT [AT]^CCGG[AT]
BsmI 383 GAATGC GAATGC(1/-1)
BspHI 3441 TCATGA T^CATGA
Bst1107I 1352 GTATAC GTA^TAC
BstAPI 2902 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1352 GTATAC GTA^TAC
DraIII 596 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4058 GACTCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2335 CGGCCG C^GGCCG
EarI 1582 GAAGAG CTCTTC(1/4)
EclXI 2335 CGGCCG C^GGCCG
Eco52I 2335 CGGCCG C^GGCCG
HindIII 3238 AAGCTT A^AGCTT
Ksp632I 1582 GAAGAG CTCTTC(1/4)
NheI 353 GCTAGC G^CTAGC
PmaCI 4123 CACGTG CAC^GTG
PmlI 4123 CACGTG CAC^GTG
PshAI 1934 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3441 TCATGA T^CATGA
SbfI 2899 CCTGCAGG CCTGCA^GG
SexAI 597 ACCTGGT A^CC[AT]GGT
SfiI 2160 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1239 CTCAAG C^T[CT][GA]AG
Sse8387I 2899 CCTGCAGG CCTGCA^GG
SspI 2985 AATATT AAT^ATT
StuI 477 AGGCCT AGG^CCT
TfiI 1435 GATTC G^A[AT]TC
XcmI 1451 CCAACTCAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 293 TGGCCA TGG^CCA
3755 TGGCCA
BcgI 334 GCAGGGATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3562 GCAGACCACTCG
BciVI 362 GGATAC GTATCC(6/5)
815 GGATAC
BlpI 432 GCTCAGC GC^T[ACGT]AGC
2324 GCTGAGC
Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC
2324 GCTGAGC
BsaAI 1770 CACGTA [CT]AC^GT[GA]
4123 CACGTG
BsaBI 739 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1647 GATGGGCATC
BsmBI 3775 CGTCTC CGTCTC(1/5)
3038 GAGACG
BsrDI 3265 CATTGC GCAATG(2/0)
3980 CATTGC
CelII 432 GCTCAGC GC^T[ACGT]AGC
2324 GCTGAGC
EcoNI 198 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2899 CCTGCAGGAGG
EcoRI 3445 GAATTC G^AATTC
3739 GAATTC
HincII 854 GTCAAC GT[CT]^[GA]AC
2957 GTTGAC
HindII 854 GTCAAC GT[CT]^[GA]AC
2957 GTTGAC
MamI 739 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1647 GATGGGCATC
MluNI 293 TGGCCA TGG^CCA
3755 TGGCCA
MscI 293 TGGCCA TGG^CCA
3755 TGGCCA
SacI 2327 GAGCTC GAGCT^C
4073 GAGCTC
SphI 630 GCATGC GCATG^C
2033 GCATGC
XbaI 3046 TCTAGA T^CTAGA
3855 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AviII BanI
BanII BbeI BbsI BbvI BclI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI