##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AclI AcyI Aor51HI
AscI Asp718I BbeI BsaHI BsiEI
BsiWI BsmBI BspDI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI Hin1I
HpaI KasI KpnI KspI MluI
NarI NotI NruI NspV PacI
PmeI PshAI Psp1406I PvuI RsrII
SacII SfiI SfoI SfuI SgrAI
SpeI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 3927 CGCG CG^CG
AccIII 3471 TCCGGA T^CCGGA
AgeI 4190 ACCGGT A^CCGGT
Alw44I 6883 GTGCAC G^TGCAC
ApaLI 6883 GTGCAC G^TGCAC
Asp700I 1016 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3667 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 285 TGCGCA TGC^GCA
BbrPI 5271 CACGTG CAC^GTG
BcgI 3118 GCAGCTTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5389 TGATCA T^GATCA
BsaBI 5991 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3471 TCCGGA T^CCGGA
BsmI 608 GAATGC GAATGC(1/-1)
Bsp1407I 1806 TGTACA T^GTACA
BspEI 3471 TCCGGA T^CCGGA
BsrGI 1806 TGTACA T^GTACA
BssSI 2039 CACGAG CACGAG(-5/-1)
BstAPI 5985 GCATCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3927 CGCG CG^CG
DraIII 5040 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 5389 TGATCA T^GATCA
FspI 285 TGCGCA TGC^GCA
HaeII 2064 AGCGCC [GA]GCGC^[CT]
HgaI 776 GACGC GACGC(5/10)
MamI 5991 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6761 CAATTG C^AATTG
MroI 3471 TCCGGA T^CCGGA
MunI 6761 CAATTG C^AATTG
MvnI 3927 CGCG CG^CG
NdeI 6790 CATATG CA^TATG
NheI 5234 GCTAGC G^CTAGC
PaeR7I 2396 CTCGAG C^TCGAG
PflFI 3667 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4190 ACCGGT A^CCGGT
PmaCI 5271 CACGTG CAC^GTG
PmlI 5271 CACGTG CAC^GTG
SalI 897 GTCGAC G^TCGAC
SmaI 63 CCCGGG CCC^GGG
SnaBI 410 TACGTA TAC^GTA
SspBI 1806 TGTACA T^GTACA
Tth111I 3667 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2396 CTCGAG C^TCGAG
XmaCI 63 CCCGGG C^CCGGG
XmaI 63 CCCGGG C^CCGGG
XmnI 1016 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 2753 CTTAAG C^TTAAG
2944 CTTAAG
AhdI 1904 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2578 GACAGAGAGTC
AocI 3023 CCTGAGG CC^T[ACGT]AGG
3151 CCTCAGG
AseI 164 ATTAAT AT^TAAT
5017 ATTAAT
AsnI 164 ATTAAT AT^TAAT
5017 ATTAAT
AspEI 1904 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2578 GACAGAGAGTC
AvaI 63 CCCGGG C^[CT]CG[GA]G
2396 CTCGAG
AvrII 2029 CCTAGG C^CTAGG
4016 CCTAGG
BamHI 94 GGATCC G^GATCC
5695 GGATCC
BbvCI 3224 GCTGAGG CCTCAGC(-5/-2)
3647 GCTGAGG
BfrI 2753 CTTAAG C^TTAAG
2944 CTTAAG
BglII 5866 AGATCT A^GATCT
6751 AGATCT
BlnI 2029 CCTAGG C^CTAGG
4016 CCTAGG
BsaI 2406 GGTCTC GGTCTC(1/5)
7174 GGTCTC
BsoBI 63 CCCGGG C^[CT]CG[GA]G
2396 CTCGAG
BspHI 1519 TCATGA T^CATGA
7138 TCATGA
BsrBI 202 CCGCTC CCGCTC(-3/-3)
50 GAGCGG
Bsu36I 3023 CCTGAGG CC^T[ACGT]AGG
3151 CCTCAGG
Eam1105I 1904 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2578 GACAGAGAGTC
Eco81I 3023 CCTGAGG CC^T[ACGT]AGG
3151 CCTCAGG
NaeI 1698 GCCGGC GCC^GGC
2034 GCCGGC
NgoMIV 1698 GCCGGC G^CCGGC
2034 GCCGGC
PshBI 164 ATTAAT AT^TAAT
5017 ATTAAT
RcaI 1519 TCATGA T^CATGA
7138 TCATGA
SacI 1382 GAGCTC GAGCT^C
3467 GAGCTC
SbfI 2791 CCTGCAGG CCTGCA^GG
3292 CCTGCAGG
SexAI 5805 ACCTGGT A^CC[AT]GGT
6925 ACCAGGT
SphI 288 GCATGC GCATG^C
3029 GCATGC
Sse8387I 2791 CCTGCAGG CCTGCA^GG
3292 CCTGCAGG
SspI 1042 AATATT AAT^ATT
4700 AATATT
XbaI 424 TCTAGA T^CTAGA
6195 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII