##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I BciVI BclI
BfrI BglII BsaBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
Eco47III EcoRI EcoRV EcoT22I FbaI
HincII HindII HindIII HpaI KpnI
MamI MfeI MunI NdeI NheI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfuI
SnaBI SpeI SphI SspBI SspI
SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 5726 GAATTT [GA]^AATT[CT]
AflIII 2253 ACGCGT A^C[GA][CT]GT
ApoI 5726 GAATTT [GA]^AATT[CT]
Asp700I 3221 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1815 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3927 CCTAGG C^CTAGG
BamHI 5175 GGATCC G^GATCC
BcgI 26 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3927 CCTAGG C^CTAGG
BsiWI 5318 CGTACG C^GTACG
BsrBI 2046 CCGCTC CCGCTC(-3/-3)
BssHII 2697 GCGCGC G^CGCGC
BssSI 2473 CTCGTG CACGAG(-5/-1)
DraIII 3618 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4991 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
FseI 3180 GGCCGGCC GGCCGG^CC
KspI 875 CCGCGG CCGC^GG
MluI 2253 ACGCGT A^CGCGT
NotI 1 GCGGCCGC GC^GGCCGC
NspI 4108 GCATGT [GA]CATG^[CT]
PflFI 1815 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3546 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 875 CCGCGG CCGC^GG
SfiI 5578 GGCCTGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2680 CGCCGGTG C[GA]^CCGG[CT]G
Tth111I 1815 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1121 TCTAGA T^CTAGA
XmnI 3221 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 396 TCCGGA T^CCGGA
821 TCCGGA
AhdI 3497 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3762 GACACAGGGTC
AspEI 3497 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3762 GACACAGGGTC
BbrPI 407 CACGTG CAC^GTG
2949 CACGTG
BlpI 673 GCTCAGC GC^T[ACGT]AGC
1069 GCTCAGC
Bpu1102I 673 GCTCAGC GC^T[ACGT]AGC
1069 GCTCAGC
BsaAI 407 CACGTG [CT]AC^GT[GA]
2949 CACGTG
BseAI 396 TCCGGA T^CCGGA
821 TCCGGA
BspEI 396 TCCGGA T^CCGGA
821 TCCGGA
BstAPI 201 GCAGGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
883 GCAGTGAGTGC
CelII 673 GCTCAGC GC^T[ACGT]AGC
1069 GCTCAGC
DraI 1721 TTTAAA TTT^AAA
4011 TTTAAA
Eam1105I 3497 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3762 GACACAGGGTC
MroI 396 TCCGGA T^CCGGA
821 TCCGGA
PaeR7I 2344 CTCGAG C^TCGAG
4289 CTCGAG
PmaCI 407 CACGTG CAC^GTG
2949 CACGTG
PmlI 407 CACGTG CAC^GTG
2949 CACGTG
SapI 3221 GAAGAGC GCTCTTC(1/4)
4088 GCTCTTC
SbfI 331 CCTGCAGG CCTGCA^GG
1703 CCTGCAGG
Sse8387I 331 CCTGCAGG CCTGCA^GG
1703 CCTGCAGG
StuI 1363 AGGCCT AGG^CCT
2657 AGGCCT
TfiI 4319 GAATC G^A[AT]TC
4488 GAATC
XhoI 2344 CTCGAG C^TCGAG
4289 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI