##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AscI AseI AsnI BbeI
BbrPI BclI BfrI BsaAI BsaHI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BsrFI BsrGI BssHII BstUI Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco52I FauI FbaI FseI Hin1I
KasI KspI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AflIII 1839 ACATGT A^C[GA][CT]GT
Alw44I 2648 GTGCAC G^TGCAC
AocI 4079 CCTTAGG CC^T[ACGT]AGG
ApaLI 2648 GTGCAC G^TGCAC
Asp700I 190 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4170 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 903 TGCGCA TGC^GCA
AvrII 2514 CCTAGG C^CTAGG
BamHI 1115 GGATCC G^GATCC
BcgI 463 GCAGAAAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 7 CCGGG CC^[GC]GG
BglI 363 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3351 AGATCT A^GATCT
BlnI 2514 CCTAGG C^CTAGG
BlpI 3029 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3029 GCTGAGC GC^T[ACGT]AGC
BsaBI 1813 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1337 GCATTC GAATGC(1/-1)
BspLU11I 1839 ACATGT A^CATGT
BsrBI 1008 CCGCTC CCGCTC(-3/-3)
BssSI 2676 CTCGTG CACGAG(-5/-1)
BstAPI 859 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2365 TTCGAA TT^CGAA
BstEII 2186 GGTGACC G^GT[ACGT]ACC
BstPI 2186 GGTGACC G^GT[ACGT]ACC
Bsu36I 4079 CCTTAGG CC^T[ACGT]AGG
CelII 3029 GCTGAGC GC^T[ACGT]AGC
DraIII 2569 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3953 CCATGG C^C[GA][CT]GG
Eco81I 4079 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2186 GGTGACC G^GT[ACGT]ACC
EcoRI 3931 GAATTC G^AATTC
EcoRV 934 GATATC GAT^ATC
EcoT22I 4049 ATGCAT ATGCA^T
FspI 903 TGCGCA TGC^GCA
HgaI 712 GACGC GACGC(5/10)
HincII 3129 GTTAAC GT[CT]^[GA]AC
HindII 3129 GTTAAC GT[CT]^[GA]AC
HpaI 3129 GTTAAC GTT^AAC
MamI 1813 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 7 CCGGG CC^[GC]GG
NcoI 3953 CCATGG C^CATGG
NheI 1528 GCTAGC G^CTAGC
NsiI 4049 ATGCAT ATGCA^T
NspV 2365 TTCGAA TT^CGAA
PaeR7I 2904 CTCGAG C^TCGAG
PflFI 4170 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 721 GAAGAGC GCTCTTC(1/4)
ScaI 4116 AGTACT AGT^ACT
SexAI 2259 ACCTGGT A^CC[AT]GGT
SfuI 2365 TTCGAA TT^CGAA
SphI 1456 GCATGC GCATG^C
Tth111I 4170 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3004 TCTAGA T^CTAGA
XhoI 2904 CTCGAG C^TCGAG
XmnI 190 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 503 GGTACC G^GTACC
1541 GGTACC
AccIII 2197 TCCGGA T^CCGGA
3480 TCCGGA
Aor51HI 563 AGCGCT AGC^GCT
928 AGCGCT
Asp718I 503 GGTACC G^GTACC
1541 GGTACC
BalI 2527 TGGCCA TGG^CCA
2890 TGGCCA
BbsI 776 GAAGAC GAAGAC(2/6)
812 GTCTTC
BpuAI 776 GAAGAC GAAGAC(2/6)
812 GTCTTC
BsaI 1807 GAGACC GGTCTC(1/5)
4168 GAGACC
BsaWI 2197 TCCGGA [AT]^CCGG[AT]
3480 TCCGGA
BseAI 2197 TCCGGA T^CCGGA
3480 TCCGGA
BspEI 2197 TCCGGA T^CCGGA
3480 TCCGGA
BsrDI 511 CATTGC GCAATG(2/0)
757 CATTGC
Bst1107I 1711 GTATAC GTA^TAC
2094 GTATAC
BstXI 2806 CCAAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3887 CCAGCACTTTGG
BstZ17I 1711 GTATAC GTA^TAC
2094 GTATAC
EaeI 2527 TGGCCA [CT]^GGCC[GA]
2890 TGGCCA
Eco47III 563 AGCGCT AGC^GCT
928 AGCGCT
EcoNI 1469 CCTCAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3659 CCTTTTTGAGG
KpnI 503 GGTACC GGTAC^C
1541 GGTACC
MluNI 2527 TGGCCA TGG^CCA
2890 TGGCCA
MroI 2197 TCCGGA T^CCGGA
3480 TCCGGA
MscI 2527 TGGCCA TGG^CCA
2890 TGGCCA
NspI 1456 GCATGC [GA]CATG^[CT]
1839 ACATGT
PpuMI 337 AGGACCT [GA]G^G[AT]CC[CT]
1198 GGGTCCT
PstI 2335 CTGCAG CTGCA^G
3362 CTGCAG
SacI 1410 GAGCTC GAGCT^C
3032 GAGCTC
SmlI 2342 CTTGAG C^T[CT][GA]AG
2904 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApaI ApoI
AspEI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BciVI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII Eam1105I EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII