##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AccIII AcyI AflIII AgeI
AhdI AscI AspEI AviII BbeI
BbrPI BsaAI BsaHI BsaWI BseAI
BsiWI BspDI BspEI BspLU11I BssHII
BssSI BstBI ClaI CpoI DrdI
Eam1105I EcoRV FseI FspI Hin1I
HpaI KasI MluI MroI NarI
NotI NruI NspV PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SalI SbfI SfoI SfuI SgrAI
SnaBI Sse8387I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 7564 GGTACC G^GTACC
AclI 732 AACGTT AA^CGTT
Alw44I 4035 GTGCAC G^TGCAC
Aor51HI 429 AGCGCT AGC^GCT
ApaLI 4035 GTGCAC G^TGCAC
Asp718I 7564 GGTACC G^GTACC
BbvCI 3618 CCTCAGC CCTCAGC(-5/-2)
BcgI 7161 GCAATGAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1320 AGATCT A^GATCT
BsiEI 197 CGGCCG CG[GA][CT]^CG
Bsp1407I 7463 TGTACA T^GTACA
BspHI 4077 TCATGA T^CATGA
BsrGI 7463 TGTACA T^GTACA
Bst1107I 1489 GTATAC GTA^TAC
BstAPI 5634 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2115 CCAGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1489 GTATAC GTA^TAC
DraIII 3260 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 197 CGGCCG C^GGCCG
EclXI 197 CGGCCG C^GGCCG
Eco47III 429 AGCGCT AGC^GCT
Eco52I 197 CGGCCG C^GGCCG
EcoNI 773 CCTTCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 6677 GAATTC G^AATTC
HaeII 429 AGCGCT [GA]GCGC^[CT]
KpnI 7564 GGTACC GGTAC^C
MfeI 1787 CAATTG C^AATTG
MunI 1787 CAATTG C^AATTG
NdeI 4040 CATATG CA^TATG
NheI 6555 GCTAGC G^CTAGC
PaeR7I 777 CTCGAG C^TCGAG
Psp1406I 732 AACGTT AA^CGTT
RcaI 4077 TCATGA T^CATGA
SacI 5652 GAGCTC GAGCT^C
SexAI 6667 ACCTGGT A^CC[AT]GGT
SfiI 80 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 313 CCCGGG CCC^GGG
SpeI 7094 ACTAGT A^CTAGT
SspBI 7463 TGTACA T^GTACA
StuI 2177 AGGCCT AGG^CCT
SwaI 7395 ATTTAAAT ATTT^AAAT
XbaI 3626 TCTAGA T^CTAGA
XhoI 777 CTCGAG C^TCGAG
XmaCI 313 CCCGGG C^CCGGG
XmaI 313 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1056 GTCTAC GT^[AC][GT]AC
1489 GTATAC
Asp700I 4516 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5164 GAAAATGTTC
AspI 1970 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5952 GACATGGTC
AvrII 2907 CCTAGG C^CTAGG
4593 CCTAGG
BalI 2420 TGGCCA TGG^CCA
3092 TGGCCA
BamHI 1299 GGATCC G^GATCC
7571 GGATCC
BlnI 2907 CCTAGG C^CTAGG
4593 CCTAGG
BlpI 4630 GCTGAGC GC^T[ACGT]AGC
7330 GCTGAGC
Bpu1102I 4630 GCTGAGC GC^T[ACGT]AGC
7330 GCTGAGC
BsaBI 1511 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2096 GATCAGTATC
BsaI 2756 GAGACC GGTCTC(1/5)
5783 GAGACC
BsrBI 63 CCGCTC CCGCTC(-3/-3)
1331 GAGCGG
BsrFI 81 GCCGGC [GA]^CCGG[CT]
2076 GCCGGC
BstEII 4205 GGTGACC G^GT[ACGT]ACC
6853 GGTCACC
BstPI 4205 GGTGACC G^GT[ACGT]ACC
6853 GGTCACC
CelII 4630 GCTGAGC GC^T[ACGT]AGC
7330 GCTGAGC
Cfr10I 81 GCCGGC [GA]^CCGG[CT]
2076 GCCGGC
EcoO65I 4205 GGTGACC G^GT[ACGT]ACC
6853 GGTCACC
HindIII 577 AAGCTT A^AGCTT
1997 AAGCTT
MamI 1511 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2096 GATCAGTATC
MluNI 2420 TGGCCA TGG^CCA
3092 TGGCCA
MscI 2420 TGGCCA TGG^CCA
3092 TGGCCA
NaeI 81 GCCGGC GCC^GGC
2076 GCCGGC
NcoI 2849 CCATGG C^CATGG
3222 CCATGG
NgoMIV 81 GCCGGC G^CCGGC
2076 GCCGGC
PacI 6515 TTAATTAA TTAAT^TAA
6534 TTAATTAA
PflFI 1970 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5952 GACATGGTC
ScaI 32 AGTACT AGT^ACT
712 AGTACT
SphI 3365 GCATGC GCATG^C
7657 GCATGC
Tth111I 1970 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5952 GACATGGTC
XmnI 4516 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5164 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI AlwNI AocI ApaI
ApoI AseI AsnI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BfrI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PshBI PstI PvuII RsaI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII