##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AspEI AviII AvrII BbrPI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsiWI BspDI BspEI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
Eam1105I Eco47III Eco81I EcoO65I FseI
FspI HindIII HpaI MfeI MluI
MroI MunI NruI PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SalI SbfI SexAI SgrAI
SnaBI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 570 GGTACC G^GTACC
AccI 3030 GTAGAC GT^[AC][GT]AC
AcyI 287 GGCGCC G[GA]^CG[CT]C
AflIII 756 ACATGT A^C[GA][CT]GT
ApaI 3258 GGGCCC GGGCC^C
Asp718I 570 GGTACC G^GTACC
AspI 1747 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1875 TGGCCA TGG^CCA
BbeI 287 GGCGCC GGCGC^C
BbsI 3944 GTCTTC GAAGAC(2/6)
BbvCI 1537 CCTCAGC CCTCAGC(-5/-2)
BcgI 898 GCAAGGAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4851 GTATCC GTATCC(6/5)
BclI 2061 TGATCA T^GATCA
BpuAI 3944 GTCTTC GAAGAC(2/6)
BsaBI 2105 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 287 GGCGCC G[GA]^CG[CT]C
BsiEI 96 CGGCCG CG[GA][CT]^CG
BsmBI 731 GAGACG CGTCTC(1/5)
BspLU11I 756 ACATGT A^CATGT
BsrBI 37 GAGCGG CCGCTC(-3/-3)
BstAPI 1115 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 361 GACCCGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 96 CGGCCG C^GGCCG
EclXI 96 CGGCCG C^GGCCG
Eco52I 96 CGGCCG C^GGCCG
EcoT22I 2930 ATGCAT ATGCA^T
FbaI 2061 TGATCA T^GATCA
Hin1I 287 GGCGCC G[GA]^CG[CT]C
KasI 287 GGCGCC G^GCGCC
KpnI 570 GGTACC GGTAC^C
MamI 2105 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1875 TGGCCA TGG^CCA
MscI 1875 TGGCCA TGG^CCA
NarI 287 GGCGCC GG^CGCC
NdeI 5165 CATATG CA^TATG
NheI 4253 GCTAGC G^CTAGC
NotI 95 GCGGCCGC GC^GGCCGC
NsiI 2930 ATGCAT ATGCA^T
PaeR7I 187 CTCGAG C^TCGAG
PflFI 1747 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 2642 GAAGAGC GCTCTTC(1/4)
SfiI 3259 GGCCCCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 287 GGCGCC GGC^GCC
SmaI 2356 CCCGGG CCC^GGG
SpeI 5404 ACTAGT A^CTAGT
Tth111I 1747 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 187 CTCGAG C^TCGAG
XmaCI 2356 CCCGGG C^CCGGG
XmaI 2356 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 3861 CTTAAG C^TTAAG
4334 CTTAAG
AseI 4450 ATTAAT AT^TAAT
5091 ATTAAT
AsnI 4450 ATTAAT AT^TAAT
5091 ATTAAT
Asp700I 1528 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3607 GAAAAAGTTC
BfrI 3861 CTTAAG C^TTAAG
4334 CTTAAG
BpmI 762 CTCCAG CTGGAG(16/14)
2867 CTCCAG
BsmI 3109 GAATGC GAATGC(1/-1)
2932 GCATTC
Bsp1407I 1275 TGTACA T^GTACA
3187 TGTACA
BspHI 3873 TCATGA T^CATGA
4909 TCATGA
BsrGI 1275 TGTACA T^GTACA
3187 TGTACA
BstBI 2541 TTCGAA TT^CGAA
3604 TTCGAA
DraI 5281 TTTAAA TTT^AAA
5466 TTTAAA
EcoNI 843 CCTTAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
970 CCTTGACCAGG
HaeII 203 AGCGCC [GA]GCGC^[CT]
287 GGCGCC
HincII 920 GTTGAC GT[CT]^[GA]AC
1744 GTTGAC
HindII 920 GTTGAC GT[CT]^[GA]AC
1744 GTTGAC
NspV 2541 TTCGAA TT^CGAA
3604 TTCGAA
PshAI 185 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1089 GACCAAGGTC
PshBI 4450 ATTAAT AT^TAAT
5091 ATTAAT
RcaI 3873 TCATGA T^CATGA
4909 TCATGA
ScaI 933 AGTACT AGT^ACT
4673 AGTACT
SfuI 2541 TTCGAA TT^CGAA
3604 TTCGAA
SspBI 1275 TGTACA T^GTACA
3187 TGTACA
SspI 492 AATATT AAT^ATT
647 AATATT
StuI 1695 AGGCCT AGG^CCT
4876 AGGCCT
XbaI 1012 TCTAGA T^CTAGA
5079 TCTAGA
XmnI 1528 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3607 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BamHI BanI BanII BbvI
BcnI BfaI BglI BmyI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII