##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII BamHI BbrPI BlpI
Bpu1102I BsiEI BsiWI BspDI BsrBI
BssHII BstBI CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FseI FspI KpnI KspI
MfeI MluI MunI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI RsrII SacII SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SphI Sse8387I Tth111I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 664 GACGTC GACGT^C
AccII 7436 CGCG CG^CG
AccIII 818 TCCGGA T^CCGGA
BseAI 818 TCCGGA T^CCGGA
BsmBI 211 GAGACG CGTCTC(1/5)
Bsp1407I 2402 TGTACA T^GTACA
BspEI 818 TCCGGA T^CCGGA
BsrFI 1097 GCCGGC [GA]^CCGG[CT]
BsrGI 2402 TGTACA T^GTACA
BssSI 421 CTCGTG CACGAG(-5/-1)
BstAPI 397 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 258 GGTGACC G^GT[ACGT]ACC
BstPI 258 GGTGACC G^GT[ACGT]ACC
BstUI 7436 CGCG CG^CG
Cfr10I 1097 GCCGGC [GA]^CCGG[CT]
EcoNI 4660 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 258 GGTGACC G^GT[ACGT]ACC
FauI 7275 GCGGG CCCGC(4/6)
HpaI 7160 GTTAAC GTT^AAC
MroI 818 TCCGGA T^CCGGA
MvnI 7436 CGCG CG^CG
NaeI 1097 GCCGGC GCC^GGC
NgoMIV 1097 GCCGGC G^CCGGC
SexAI 8000 ACCAGGT A^CC[AT]GGT
SmaI 570 CCCGGG CCC^GGG
SpeI 5451 ACTAGT A^CTAGT
SspBI 2402 TGTACA T^GTACA
XmaCI 570 CCCGGG C^CCGGG
XmaI 570 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaI 570 CCCGGG C^[CT]CG[GA]G
704 CCCGAG
AvrII 3988 CCTAGG C^CTAGG
8379 CCTAGG
BalI 5111 TGGCCA TGG^CCA
8215 TGGCCA
BbeI 7377 GGCGCC GGCGC^C
8277 GGCGCC
BcgI 603 CGAAGAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
330 GCAGGCCAATCG
BlnI 3988 CCTAGG C^CTAGG
8379 CCTAGG
BsaAI 286 TACGTG [CT]AC^GT[GA]
635 TACGTG
BsmI 6278 GAATGC GAATGC(1/-1)
1485 GCATTC
BsoBI 570 CCCGGG C^[CT]CG[GA]G
704 CCCGAG
BspMI 2455 ACCTGC ACCTGC(4/8)
8311 GCAGGT
Bst1107I 4137 GTATAC GTA^TAC
6138 GTATAC
BstZ17I 4137 GTATAC GTA^TAC
6138 GTATAC
DraII 533 GGGGCCT [GA]G^G[ACGT]CC[CT]
2067 AGGGCCT
EaeI 5111 TGGCCA [CT]^GGCC[GA]
8215 TGGCCA
EcoO109I 533 GGGGCCT [GA]G^G[ACGT]CC[CT]
2067 AGGGCCT
EcoRI 933 GAATTC G^AATTC
2374 GAATTC
HaeII 7377 GGCGCC [GA]GCGC^[CT]
8277 GGCGCC
KasI 7377 GGCGCC G^GCGCC
8277 GGCGCC
MluNI 5111 TGGCCA TGG^CCA
8215 TGGCCA
MscI 5111 TGGCCA TGG^CCA
8215 TGGCCA
NarI 7377 GGCGCC GG^CGCC
8277 GGCGCC
PflMI 193 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
582 CCAGGCCTTGG
ScaI 2661 AGTACT AGT^ACT
2671 AGTACT
SfoI 7377 GGCGCC GGC^GCC
8277 GGCGCC
SwaI 2309 ATTTAAAT ATTT^AAAT
7796 ATTTAAAT
Van91I 193 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
582 CCAGGCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AciI AclI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI AocI ApoI AseI AsnI
Asp700I AspHI AvaII BanI BanII
BbsI BbvCI BbvI BciVI BclI
BcnI BfaI BfrI BglI BglII
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraIII DsaI EarI
Eco81I EcoRII EcoRV EcoT14I EcoT22I
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI Psp1406I PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI