##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AvrII BbeI BlnI
BsaWI BseAI BspDI BspEI BsrFI
BssHII BstAPI BstBI Bsu36I Cfr10I
ClaI CpoI DraIII Eam1105I Eco47III
Eco81I FseI KasI KpnI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PvuI RsrII SacI
SalI SbfI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 99 GACGCC G[GA]^CG[CT]C
ApaI 270 GGGCCC GGGCC^C
AviII 226 TGCGCA TGC^GCA
BamHI 1886 GGATCC G^GATCC
BanI 344 GGTGCC G^G[CT][GA]CC
BbvCI 7306 GCTGAGG CCTCAGC(-5/-2)
BglII 400 AGATCT A^GATCT
BsaBI 2938 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 99 GACGCC G[GA]^CG[CT]C
BsiEI 367 CGGCCG CG[GA][CT]^CG
BsiWI 160 CGTACG C^GTACG
BsmBI 3501 GAGACG CGTCTC(1/5)
BspHI 5007 TCATGA T^CATGA
BspMI 2131 ACCTGC ACCTGC(4/8)
Bst1107I 2986 GTATAC GTA^TAC
BstEII 7383 GGTGACC G^GT[ACGT]ACC
BstPI 7383 GGTGACC G^GT[ACGT]ACC
BstXI 4272 CCATATATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2986 GTATAC GTA^TAC
DrdI 110 GACCGCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 367 CGGCCG C^GGCCG
EclXI 367 CGGCCG C^GGCCG
Eco52I 367 CGGCCG C^GGCCG
EcoO65I 7383 GGTGACC G^GT[ACGT]ACC
EcoRI 1751 GAATTC G^AATTC
EcoRV 4818 GATATC GAT^ATC
EcoT22I 3772 ATGCAT ATGCA^T
FspI 226 TGCGCA TGC^GCA
HaeII 6716 GGCGCT [GA]GCGC^[CT]
Hin1I 99 GACGCC G[GA]^CG[CT]C
HpaI 7566 GTTAAC GTT^AAC
MamI 2938 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4038 CATATG CA^TATG
NsiI 3772 ATGCAT ATGCA^T
PaeR7I 3779 CTCGAG C^TCGAG
PpuMI 902 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 5007 TCATGA T^CATGA
SapI 3247 GAAGAGC GCTCTTC(1/4)
SmaI 273 CCCGGG CCC^GGG
XhoI 3779 CTCGAG C^TCGAG
XmaCI 273 CCCGGG C^CCGGG
XmaI 273 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AclI 4263 AACGTT AA^CGTT
7508 AACGTT
AseI 1280 ATTAAT AT^TAAT
4733 ATTAAT
AsnI 1280 ATTAAT AT^TAAT
4733 ATTAAT
AspI 1320 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2111 GACACTGTC
BanII 128 GAGCCC G[GA]GC[CT]^C
270 GGGCCC
BbrPI 5241 CACGTG CAC^GTG
5338 CACGTG
BcgI 1954 GCATCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2162 GCAAAATGATCG
BglI 130 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6752 GCCCCACGGGC
BsmI 2056 GAATGC GAATGC(1/-1)
5560 GAATGC
Bsp1407I 759 TGTACA T^GTACA
4960 TGTACA
BspLU11I 4207 ACATGT A^CATGT
6837 ACATGT
BsrBI 378 GAGCGG CCGCTC(-3/-3)
6566 GAGCGG
BsrGI 759 TGTACA T^GTACA
4960 TGTACA
HgaI 99 GACGC GACGC(5/10)
409 GCGTC
KspI 133 CCGCGG CCGC^GG
287 CCGCGG
PflFI 1320 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2111 GACACTGTC
PmaCI 5241 CACGTG CAC^GTG
5338 CACGTG
PmlI 5241 CACGTG CAC^GTG
5338 CACGTG
PshBI 1280 ATTAAT AT^TAAT
4733 ATTAAT
Psp1406I 4263 AACGTT AA^CGTT
7508 AACGTT
SacII 133 CCGCGG CCGC^GG
287 CCGCGG
ScaI 4919 AGTACT AGT^ACT
7667 AGTACT
SexAI 1697 ACCAGGT A^CC[AT]GGT
4060 ACCAGGT
SnaBI 3224 TACGTA TAC^GTA
4196 TACGTA
SspBI 759 TGTACA T^GTACA
4960 TGTACA
Tth111I 1320 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2111 GACACTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BalI BbsI BbvI BciVI BclI
BcnI BfaI BfrI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI