##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp718I AspEI AvaI
AviII AvrII BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaHI BsiEI BsiWI
BsoBI BspDI BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I KasI KpnI
KspI MluI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I StuI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 3322 CGCG CG^CG
AccIII 2906 TCCGGA T^CCGGA
AspI 5996 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 3085 GGCACC G^G[CT][GA]CC
BsaI 6035 GGTCTC GGTCTC(1/5)
BsaWI 2906 TCCGGA [AT]^CCGG[AT]
BseAI 2906 TCCGGA T^CCGGA
BseRI 5394 GAGGAG GAGGAG(10/8)
BsgI 2366 GTGCAG GTGCAG(16/14)
BsmBI 1636 GAGACG CGTCTC(1/5)
Bsp1407I 5091 TGTACA T^GTACA
BspEI 2906 TCCGGA T^CCGGA
BspMI 1691 GCAGGT ACCTGC(4/8)
BsrFI 5051 ACCGGC [GA]^CCGG[CT]
BsrGI 5091 TGTACA T^GTACA
BstEII 2532 GGTAACC G^GT[ACGT]ACC
BstPI 2532 GGTAACC G^GT[ACGT]ACC
BstUI 3322 CGCG CG^CG
Cfr10I 5051 ACCGGC [GA]^CCGG[CT]
DraIII 3011 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 751 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2532 GGTAACC G^GT[ACGT]ACC
EcoRV 2143 GATATC GAT^ATC
FauI 4867 CCCGC CCCGC(4/6)
HindIII 1562 AAGCTT A^AGCTT
MroI 2906 TCCGGA T^CCGGA
MspA1I 1671 CAGCTG C[AC]G^C[GT]G
MvnI 3322 CGCG CG^CG
PflFI 5996 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5239 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2632 CTGCAG CTGCA^G
PvuII 1671 CAGCTG CAG^CTG
SacI 625 GAGCTC GAGCT^C
SexAI 6145 ACCTGGT A^CC[AT]GGT
SspBI 5091 TGTACA T^GTACA
Tth111I 5996 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 6199 CCAAAAAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 682 ACATGT A^C[GA][CT]GT
5120 ACATGT
Alw44I 1936 GTGCAC G^TGCAC
5979 GTGCAC
ApaLI 1936 GTGCAC G^TGCAC
5979 GTGCAC
AseI 1983 ATTAAT AT^TAAT
4726 ATTAAT
AsnI 1983 ATTAAT AT^TAAT
4726 ATTAAT
BalI 4373 TGGCCA TGG^CCA
4522 TGGCCA
BamHI 2741 GGATCC G^GATCC
3924 GGATCC
BanII 625 GAGCTC G[GA]GC[CT]^C
4240 GGGCTC
BclI 2861 TGATCA T^GATCA
6029 TGATCA
BsaBI 95 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
256 GATGTAAATC
BspHI 2147 TCATGA T^CATGA
2864 TCATGA
BspLU11I 682 ACATGT A^CATGT
5120 ACATGT
BstAPI 3288 GCAAGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3670 GCAGAACATGC
BstXI 1732 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5728 CCAATGTACTGG
Cac8I 5269 GCTGGC GC[ACGT]^[ACGT]GC
6535 GCAAGC
DsaI 933 CCATGG C^C[GA][CT]GG
6372 CCATGG
EaeI 4373 TGGCCA [CT]^GGCC[GA]
4522 TGGCCA
EcoT22I 3446 ATGCAT ATGCA^T
4615 ATGCAT
FbaI 2861 TGATCA T^GATCA
6029 TGATCA
HapII 2907 CCGG C^CGG
5052 CCGG
HpaI 5203 GTTAAC GTT^AAC
6522 GTTAAC
HpaII 2907 CCGG C^CGG
5052 CCGG
MamI 95 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
256 GATGTAAATC
MfeI 4634 CAATTG C^AATTG
4812 CAATTG
MluNI 4373 TGGCCA TGG^CCA
4522 TGGCCA
MscI 4373 TGGCCA TGG^CCA
4522 TGGCCA
MspI 2907 CCGG C^CGG
5052 CCGG
MunI 4634 CAATTG C^AATTG
4812 CAATTG
NcoI 933 CCATGG C^CATGG
6372 CCATGG
NdeI 2981 CATATG CA^TATG
4612 CATATG
NsiI 3446 ATGCAT ATGCA^T
4615 ATGCAT
PshBI 1983 ATTAAT AT^TAAT
4726 ATTAAT
RcaI 2147 TCATGA T^CATGA
2864 TCATGA
SapI 2968 GCTCTTC GCTCTTC(1/4)
5079 GCTCTTC
SnaBI 2575 TACGTA TAC^GTA
5784 TACGTA
XbaI 182 TCTAGA T^CTAGA
6446 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BbsI BbvI BciVI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraI DrdI EarI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI