##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AviII BbeI BbrPI
BcgI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
BssSI BstUI Bsu36I CelII Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I Eco81I FseI FspI HaeII
Hin1I HpaI KasI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SalI SfiI
SfoI SgrAI SmaI SnaBI SphI
StuI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 3444 GGTACC G^GTACC
AclI 1411 AACGTT AA^CGTT
AhdI 338 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3444 GGTACC G^GTACC
AspEI 338 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1768 GAGCAC G[AT]GC[AT]^C
AvaI 236 CCCGAG C^[CT]CG[GA]G
BanII 1174 GAGCCC G[GA]GC[CT]^C
BbvCI 813 CCTCAGC CCTCAGC(-5/-2)
BciVI 3282 GTATCC GTATCC(6/5)
BglI 1111 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI 1768 GAGCAC G[AT]GC[AT]^C
BsmBI 4763 GAGACG CGTCTC(1/5)
BsoBI 236 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3195 TGTACA T^GTACA
BspMI 5605 GCAGGT ACCTGC(4/8)
BsrGI 3195 TGTACA T^GTACA
Bst1107I 5048 GTATAC GTA^TAC
BstBI 759 TTCGAA TT^CGAA
BstZ17I 5048 GTATAC GTA^TAC
DrdI 2106 GACAGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 338 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1892 GAATTC G^AATTC
HgaI 5771 GCGTC GACGC(5/10)
HincII 3945 GTTGAC GT[CT]^[GA]AC
HindII 3945 GTTGAC GT[CT]^[GA]AC
KpnI 3444 GGTACC GGTAC^C
NheI 2244 GCTAGC G^CTAGC
NspV 759 TTCGAA TT^CGAA
PflMI 3432 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1411 AACGTT AA^CGTT
SapI 278 GAAGAGC GCTCTTC(1/4)
SbfI 1343 CCTGCAGG CCTGCA^GG
ScaI 5689 AGTACT AGT^ACT
SexAI 539 ACCAGGT A^CC[AT]GGT
SfuI 759 TTCGAA TT^CGAA
SpeI 4056 ACTAGT A^CTAGT
Sse8387I 1343 CCTGCAGG CCTGCA^GG
SspBI 3195 TGTACA T^GTACA
SwaI 6322 ATTTAAAT ATTT^AAAT
Van91I 3432 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 324 CTTAAG C^TTAAG
1284 CTTAAG
AflIII 2641 ACATGT A^C[GA][CT]GT
4774 ACATGT
AseI 1290 ATTAAT AT^TAAT
4376 ATTAAT
AsnI 1290 ATTAAT AT^TAAT
4376 ATTAAT
AvrII 711 CCTAGG C^CTAGG
5909 CCTAGG
BfrI 324 CTTAAG C^TTAAG
1284 CTTAAG
BglII 2978 AGATCT A^GATCT
4991 AGATCT
BlnI 711 CCTAGG C^CTAGG
5909 CCTAGG
BseRI 358 CTCCTC GAGGAG(10/8)
5178 GAGGAG
BspLU11I 2641 ACATGT A^CATGT
4774 ACATGT
BstAPI 1307 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3528 GCAGAAATTGC
BstEII 3557 GGTCACC G^GT[ACGT]ACC
4744 GGTCACC
BstPI 3557 GGTCACC G^GT[ACGT]ACC
4744 GGTCACC
BstXI 1828 CCACGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2745 CCACATTTATGG
CfoI 4334 GCGC GCG^C
5597 GCGC
DraIII 4024 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
6145 CACAAAGTG
EcoO65I 3557 GGTCACC G^GT[ACGT]ACC
4744 GGTCACC
FauI 3111 CCCGC CCCGC(4/6)
6131 CCCGC
HhaI 4334 GCGC GCG^C
5597 GCGC
HinP1I 4334 GCGC G^CGC
5597 GCGC
MfeI 1683 CAATTG C^AATTG
6272 CAATTG
MspA1I 1124 CAGCTG C[AC]G^C[GT]G
2684 CAGCTG
MunI 1683 CAATTG C^AATTG
6272 CAATTG
NdeI 4884 CATATG CA^TATG
5938 CATATG
PshBI 1290 ATTAAT AT^TAAT
4376 ATTAAT
PvuII 1124 CAGCTG CAG^CTG
2684 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspI AvaII
BalI BamHI BanI BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoRV
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflFI PleI PpuMI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XcmI
XhoII XmnI