##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI AcsI AflII Alw44I
AocI Aor51HI ApaLI ApoI AseI
AsnI AspI BbsI BciVI BfrI
BglII BpuAI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI Eco47III Eco81I
EcoRI EcoRV EcoT22I HindIII HpaI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PmeI PshAI
PshBI Psp1406I PvuI RsrII SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 688 GACGTC GACGT^C
AgeI 1728 ACCGGT A^CCGGT
AhdI 524 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 300 GGCGCGCC GG^CGCGCC
Asp700I 3678 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 524 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2351 CACGTG CAC^GTG
BcgI 482 GCAGAGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 576 TGATCA T^GATCA
BlpI 3552 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3552 GCTAAGC GC^T[ACGT]AGC
BsaAI 2351 CACGTG [CT]AC^GT[GA]
BsaI 631 GAGACC GGTCTC(1/5)
BstEII 622 GGTCACC G^GT[ACGT]ACC
BstPI 622 GGTCACC G^GT[ACGT]ACC
CelII 3552 GCTAAGC GC^T[ACGT]AGC
DraI 4446 TTTAAA TTT^AAA
DrdI 1453 GACGGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 524 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 622 GGTCACC G^GT[ACGT]ACC
FbaI 576 TGATCA T^GATCA
FseI 916 GGCCGGCC GGCCGG^CC
MseI 4447 TTAA T^TAA
NspI 3131 GCATGC [GA]CATG^[CT]
PaeR7I 2582 CTCGAG C^TCGAG
PinAI 1728 ACCGGT A^CCGGT
PmaCI 2351 CACGTG CAC^GTG
PmlI 2351 CACGTG CAC^GTG
SalI 2363 GTCGAC G^TCGAC
SapI 2854 GCTCTTC GCTCTTC(1/4)
SfiI 2644 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3131 GCATGC GCATG^C
SspI 4473 AATATT AAT^ATT
Tru9I 4447 TTAA T^TAA
Tsp509I 3679 AATT ^AATT
XbaI 3335 TCTAGA T^CTAGA
XcmI 3661 CCACCCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2582 CTCGAG C^TCGAG
XmnI 3678 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 1609 GGTACC G^GTACC
3436 GGTACC
AccI 2363 GTCGAC GT^[AC][GT]AC
2423 GTAGAC
AflIII 1470 ACGTGT A^C[GA][CT]GT
2352 ACGTGT
Asp718I 1609 GGTACC G^GTACC
3436 GGTACC
AviII 786 TGCGCA TGC^GCA
2145 TGCGCA
BamHI 933 GGATCC G^GATCC
2472 GGATCC
BsiEI 284 CGGCCG CG[GA][CT]^CG
361 CGGCCG
BspHI 1332 TCATGA T^CATGA
1851 TCATGA
BssSI 1781 CACGAG CACGAG(-5/-1)
2270 CACGAG
BstXI 1950 CCAGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3409 CCATGGCCCTGG
DraIII 1050 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1150 CACCGAGTG
EagI 284 CGGCCG C^GGCCG
361 CGGCCG
EarI 2159 CTCTTC CTCTTC(1/4)
2855 CTCTTC
EclXI 284 CGGCCG C^GGCCG
361 CGGCCG
Eco52I 284 CGGCCG C^GGCCG
361 CGGCCG
FspI 786 TGCGCA TGC^GCA
2145 TGCGCA
HincII 584 GTCAAC GT[CT]^[GA]AC
2363 GTCGAC
HindII 584 GTCAAC GT[CT]^[GA]AC
2363 GTCGAC
KpnI 1609 GGTACC GGTAC^C
3436 GGTACC
Ksp632I 2159 CTCTTC CTCTTC(1/4)
2855 CTCTTC
PvuII 2138 CAGCTG CAG^CTG
2860 CAGCTG
RcaI 1332 TCATGA T^CATGA
1851 TCATGA
SacI 1907 GAGCTC GAGCT^C
2508 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI