##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AgeI AhdI AocI
AspEI AspI AvrII BbrPI BlnI
BlpI Bpu1102I BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI DraIII DrdI Eam1105I Eco81I
FseI HpaI MluI MroI NdeI
NheI NotI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PvuI
RcaI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
Tth111I
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1748 GGTACC G^GTACC
AccI 1115 GTCGAC GT^[AC][GT]AC
AclI 3564 AACGTT AA^CGTT
AflII 4276 CTTAAG C^TTAAG
Alw44I 2532 GTGCAC G^TGCAC
ApaLI 2532 GTGCAC G^TGCAC
AscI 1187 GGCGCGCC GG^CGCGCC
AseI 3593 ATTAAT AT^TAAT
AsnI 3593 ATTAAT AT^TAAT
Asp718I 1748 GGTACC G^GTACC
BcgI 2822 CGAAGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 979 TGATCA T^GATCA
BfrI 4276 CTTAAG C^TTAAG
BglII 3861 AGATCT A^GATCT
BsaAI 1823 CACGTA [CT]AC^GT[GA]
BsaBI 2001 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2420 TCCGGT [AT]^CCGG[AT]
BspLU11I 3945 ACATGT A^CATGT
BssSI 1770 CTCGTG CACGAG(-5/-1)
CpoI 243 CGGACCG CG^G[AT]CCG
EcoNI 987 CCTTTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3991 GAATTC G^AATTC
EcoRV 3172 GATATC GAT^ATC
EcoT22I 3220 ATGCAT ATGCA^T
FbaI 979 TGATCA T^GATCA
HincII 1115 GTCGAC GT[CT]^[GA]AC
HindII 1115 GTCGAC GT[CT]^[GA]AC
HindIII 3290 AAGCTT A^AGCTT
KpnI 1748 GGTACC GGTAC^C
MamI 2001 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3770 CAATTG C^AATTG
MunI 3770 CAATTG C^AATTG
NcoI 1612 CCATGG C^CATGG
NruI 1134 TCGCGA TCG^CGA
NsiI 3220 ATGCAT ATGCA^T
PshAI 1259 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3593 ATTAAT AT^TAAT
Psp1406I 3564 AACGTT AA^CGTT
RsrII 243 CGGACCG CG^G[AT]CCG
SalI 1115 GTCGAC G^TCGAC
ScaI 3187 AGTACT AGT^ACT
StuI 2651 AGGCCT AGG^CCT
SwaI 945 ATTTAAAT ATTT^AAAT
XbaI 3858 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 1779 GGGCCC GGGCC^C
2380 GGGCCC
Asp700I 76 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1964 GAACGACTTC
BalI 1609 TGGCCA TGG^CCA
2849 TGGCCA
BbvCI 297 CCTCAGC CCTCAGC(-5/-2)
1125 GCTGAGG
BciVI 3507 GGATAC GTATCC(6/5)
3123 GTATCC
BsaI 490 GAGACC GGTCTC(1/5)
713 GGTCTC
BsgI 2544 CTGCAC GTGCAG(16/14)
828 GTGCAG
BsmBI 1399 CGTCTC CGTCTC(1/5)
1365 GAGACG
BssHII 398 GCGCGC G^CGCGC
1188 GCGCGC
BstEII 1439 GGTTACC G^GT[ACGT]ACC
1868 GGTGACC
BstPI 1439 GGTTACC G^GT[ACGT]ACC
1868 GGTGACC
EagI 405 CGGCCG C^GGCCG
2078 CGGCCG
EclXI 405 CGGCCG C^GGCCG
2078 CGGCCG
Eco52I 405 CGGCCG C^GGCCG
2078 CGGCCG
EcoO65I 1439 GGTTACC G^GT[ACGT]ACC
1868 GGTGACC
EcoT14I 775 CCTTGG C^C[AT][AT]GG
1612 CCATGG
MluNI 1609 TGGCCA TGG^CCA
2849 TGGCCA
MscI 1609 TGGCCA TGG^CCA
2849 TGGCCA
PflMI 1333 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2186 CCAGCGCCTGG
PleI 1089 GACTC GAGTC(4/5)
2142 GACTC
PpuMI 205 GGGACCC [GA]G^G[AT]CC[CT]
1080 GGGTCCC
SacI 165 GAGCTC GAGCT^C
1950 GAGCTC
SphI 1876 GCATGC GCATG^C
3222 GCATGC
SspI 4189 AATATT AAT^ATT
4324 AATATT
StyI 775 CCTTGG C^C[AT][AT]GG
1612 CCATGG
TfiI 1033 GATTC G^A[AT]TC
1236 GATTC
Van91I 1333 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2186 CCAGCGCCTGG
XmnI 76 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1964 GAACGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApoI AspHI AvaI AvaII AviII
BamHI BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PstI PvuII RsaI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoI XhoII XmaCI XmaI