##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AgeI AhdI Alw44I ApaLI AscI
AspEI AviII BalI BbrPI BbvCI
BcgI BciVI BclI BglII BlpI
Bpu1102I BsiWI BsmBI BspDI BspHI
BssHII Bst1107I BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I FbaI FseI FspI MfeI
MluI MluNI MscI MunI NaeI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SgrAI SnaBI Sse8387I SwaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1152 GACGTC GACGT^C
Acc65I 3727 GGTACC G^GTACC
AccI 457 GTCTAC GT^[AC][GT]AC
AccIII 2068 TCCGGA T^CCGGA
AclI 3716 AACGTT AA^CGTT
AflII 3157 CTTAAG C^TTAAG
AflIII 1348 ACATGT A^C[GA][CT]GT
AocI 107 CCTGAGG CC^T[ACGT]AGG
AseI 2052 ATTAAT AT^TAAT
AsnI 2052 ATTAAT AT^TAAT
Asp718I 3727 GGTACC G^GTACC
AspI 1562 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2973 CCTAGG C^CTAGG
BfrI 3157 CTTAAG C^TTAAG
BlnI 2973 CCTAGG C^CTAGG
BsaAI 588 TACGTG [CT]AC^GT[GA]
BsaBI 1767 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2068 TCCGGA [AT]^CCGG[AT]
BseAI 2068 TCCGGA T^CCGGA
BsgI 1062 CTGCAC GTGCAG(16/14)
BsiEI 557 CGACCG CG[GA][CT]^CG
BsmI 3361 GAATGC GAATGC(1/-1)
BspEI 2068 TCCGGA T^CCGGA
BspLU11I 1348 ACATGT A^CATGT
BspMI 3390 ACCTGC ACCTGC(4/8)
BsrDI 3190 CATTGC GCAATG(2/0)
BsrFI 544 ACCGGC [GA]^CCGG[CT]
BssSI 2856 CTCGTG CACGAG(-5/-1)
BstBI 1813 TTCGAA TT^CGAA
BstXI 3108 CCAATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 107 CCTGAGG CC^T[ACGT]AGG
Cfr10I 544 ACCGGC [GA]^CCGG[CT]
EaeI 50 TGGCCG [CT]^GGCC[GA]
Eco81I 107 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1902 ATGCAT ATGCA^T
HgaI 455 GCGTC GACGC(5/10)
HindIII 2691 AAGCTT A^AGCTT
KpnI 3727 GGTACC GGTAC^C
MamI 1767 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2068 TCCGGA T^CCGGA
NcoI 1678 CCATGG C^CATGG
NdeI 1755 CATATG CA^TATG
NheI 1841 GCTAGC G^CTAGC
NsiI 1902 ATGCAT ATGCA^T
NspV 1813 TTCGAA TT^CGAA
PflFI 1562 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2052 ATTAAT AT^TAAT
Psp1406I 3716 AACGTT AA^CGTT
PstI 3516 CTGCAG CTGCA^G
SfuI 1813 TTCGAA TT^CGAA
SmaI 485 CCCGGG CCC^GGG
SpeI 3352 ACTAGT A^CTAGT
SphI 2099 GCATGC GCATG^C
Tth111I 1562 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 485 CCCGGG C^CCGGG
XmaI 485 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 196 AGCGCT AGC^GCT
221 AGCGCT
ApaI 440 GGGCCC GGGCC^C
468 GGGCCC
BbsI 1941 GAAGAC GAAGAC(2/6)
2714 GAAGAC
BpuAI 1941 GAAGAC GAAGAC(2/6)
2714 GAAGAC
BsaI 3387 GAGACC GGTCTC(1/5)
3047 GGTCTC
Bsp1407I 526 TGTACA T^GTACA
2357 TGTACA
BsrGI 526 TGTACA T^GTACA
2357 TGTACA
BstAPI 1895 GCACTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2112 GCATTATGTGC
Eco47III 196 AGCGCT AGC^GCT
221 AGCGCT
EcoRI 3574 GAATTC G^AATTC
3914 GAATTC
HpaI 1491 GTTAAC GTT^AAC
2003 GTTAAC
KspI 53 CCGCGG CCGC^GG
360 CCGCGG
PflMI 1796 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1976 CCAACTATTGG
PleI 3316 GACTC GAGTC(4/5)
654 GAGTC
PpuMI 805 AGGACCC [GA]G^G[AT]CC[CT]
829 AGGACCT
SacI 317 GAGCTC GAGCT^C
3168 GAGCTC
SacII 53 CCGCGG CCGC^GG
360 CCGCGG
SmlI 3157 CTTAAG C^T[CT][GA]AG
3909 CTTGAG
SspBI 526 TGTACA T^GTACA
2357 TGTACA
SspI 1050 AATATT AAT^ATT
3875 AATATT
StuI 265 AGGCCT AGG^CCT
2852 AGGCCT
Tsp45I 174 GTGAC ^GT[GC]AC
3868 GTGAC
Van91I 1796 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1976 CCAACTATTGG
XbaI 3578 TCTAGA T^CTAGA
3963 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BamHI BanI
BanII BbeI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmnI