##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I AviII AvrII
BamHI BciVI BclI BfrI BlnI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI DrdI
Eco47III Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
MamI MfeI MluI MroI MslI
MunI NcoI NdeI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 437 GGTACC G^GTACC
AhdI 784 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1457 GATCC GGATC(4/5)
Asp718I 437 GGTACC G^GTACC
AspEI 784 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2495 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1713 CACGTG CAC^GTG
BbsI 2289 GTCTTC GAAGAC(2/6)
BbvCI 2199 CCTCAGC CCTCAGC(-5/-2)
BglII 1747 AGATCT A^GATCT
BpuAI 2289 GTCTTC GAAGAC(2/6)
BsaAI 1713 CACGTG [CT]AC^GT[GA]
BsrBI 528 GAGCGG CCGCTC(-3/-3)
CpoI 93 CGGTCCG CG^G[AT]CCG
DraI 2344 TTTAAA TTT^AAA
DraIII 969 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 784 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 896 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1633 ATGCAT ATGCA^T
HincII 1364 GTCGAC GT[CT]^[GA]AC
HindII 1364 GTCGAC GT[CT]^[GA]AC
KpnI 437 GGTACC GGTAC^C
NheI 2147 GCTAGC G^CTAGC
NotI 153 GCGGCCGC GC^GGCCGC
NsiI 1633 ATGCAT ATGCA^T
PflFI 2495 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 669 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1713 CACGTG CAC^GTG
PmlI 1713 CACGTG CAC^GTG
PshAI 226 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 93 CGGTCCG CG^G[AT]CCG
SalI 1364 GTCGAC G^TCGAC
SapI 1211 GCTCTTC GCTCTTC(1/4)
ScaI 2038 AGTACT AGT^ACT
SexAI 970 ACCTGGT A^CC[AT]GGT
TfiI 2416 GAATC G^A[AT]TC
Tsp509I 2281 AATT ^AATT
Tth111I 2495 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 669 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 792 GTCTAC GT^[AC][GT]AC
1364 GTCGAC
AflIII 1712 ACACGT A^C[GA][CT]GT
1714 ACGTGT
Alw44I 966 GTGCAC G^TGCAC
1056 GTGCAC
ApaLI 966 GTGCAC G^TGCAC
1056 GTGCAC
BalI 469 TGGCCA TGG^CCA
1733 TGGCCA
BcgI 190 CGATGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
371 CGAGCCGTCTGC
BfaI 2148 CTAG C^TAG
2474 CTAG
BsaI 910 GAGACC GGTCTC(1/5)
1351 GAGACC
BsrDI 1636 CATTGC GCAATG(2/0)
2465 CATTGC
BstYI 1456 AGATCC [GA]^GATC[CT]
1747 AGATCT
MaeI 2148 CTAG C^TAG
2474 CTAG
MaeIII 516 GTGAC ^GT[ACGT]AC
1830 GTGAC
MflI 1456 AGATCC [GA]^GATC[CT]
1747 AGATCT
MluNI 469 TGGCCA TGG^CCA
1733 TGGCCA
MscI 469 TGGCCA TGG^CCA
1733 TGGCCA
MseI 1075 TTAA T^TAA
2345 TTAA
NspI 1162 ACATGC [GA]CATG^[CT]
1294 ACATGC
SacI 1110 GAGCTC GAGCT^C
1152 GAGCTC
SbfI 1541 CCTGCAGG CCTGCA^GG
1880 CCTGCAGG
SfiI 461 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1913 GGCCATCCTGGCC
Sse8387I 1541 CCTGCAGG CCTGCA^GG
1880 CCTGCAGG
Tru9I 1075 TTAA T^TAA
2345 TTAA
Tsp45I 516 GTGAC ^GT[GC]AC
1830 GTGAC
XhoII 1456 AGATCC [GA]^GATC[CT]
1747 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TseI TspRI TthHB8I XcmI XmaCI
XmaI