##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI Alw44I Aor51HI ApaI ApaLI
AscI AviII AvrII BbeI BbrPI
BcgI BclI BlnI BsiEI BsiWI
BsmBI BssHII BstEII BstPI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FbaI FseI FspI HincII
HindII HpaI KasI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1260 GACGTC GACGT^C
Acc65I 5701 GGTACC G^GTACC
AhdI 6251 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 6692 ATTAAT AT^TAAT
AsnI 6692 ATTAAT AT^TAAT
Asp718I 5701 GGTACC G^GTACC
AspEI 6251 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3523 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaBI 5455 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 266 ATCGAT AT^CGAT
BspHI 4095 TCATGA T^CATGA
BspLU11I 6863 ACATGT A^CATGT
BsrBI 5206 GAGCGG CCGCTC(-3/-3)
BssSI 1634 CACGAG CACGAG(-5/-1)
Bst1107I 6491 GTATAC GTA^TAC
BstZ17I 6491 GTATAC GTA^TAC
ClaI 266 ATCGAT AT^CGAT
DraIII 1128 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 6251 GACAGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4355 GATATC GAT^ATC
EcoT22I 3690 ATGCAT ATGCA^T
HaeII 1928 AGCGCC [GA]GCGC^[CT]
KpnI 5701 GGTACC GGTAC^C
KspI 4508 CCGCGG CCGC^GG
MamI 5455 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 3690 ATGCAT ATGCA^T
PflFI 3523 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 6692 ATTAAT AT^TAAT
RcaI 4095 TCATGA T^CATGA
SacI 5318 GAGCTC GAGCT^C
SacII 4508 CCGCGG CCGC^GG
ScaI 2883 AGTACT AGT^ACT
SmaI 5909 CCCGGG CCC^GGG
SspI 3562 AATATT AAT^ATT
StuI 4535 AGGCCT AGG^CCT
SwaI 5437 ATTTAAAT ATTT^AAAT
Tth111I 3523 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 148 CCAGAGAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 5909 CCCGGG C^CCGGG
XmaI 5909 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflII 4133 CTTAAG C^TTAAG
5708 CTTAAG
AflIII 5684 ACGTGT A^C[GA][CT]GT
6863 ACATGT
AgeI 204 ACCGGT A^CCGGT
5792 ACCGGT
AocI 517 CCTCAGG CC^T[ACGT]AGG
2211 CCTCAGG
BanI 5701 GGTACC G^G[CT][GA]CC
5849 GGCACC
BfrI 4133 CTTAAG C^TTAAG
5708 CTTAAG
BglII 3091 AGATCT A^GATCT
5157 AGATCT
BsaAI 1368 TACGTG [CT]AC^GT[GA]
4613 TACGTG
BsmI 1374 GCATTC GAATGC(1/-1)
4981 GCATTC
BstBI 1003 TTCGAA TT^CGAA
3834 TTCGAA
Bsu36I 517 CCTCAGG CC^T[ACGT]AGG
2211 CCTCAGG
Eco81I 517 CCTCAGG CC^T[ACGT]AGG
2211 CCTCAGG
EcoNI 347 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3067 CCTTTTCCAGG
NcoI 845 CCATGG C^CATGG
2943 CCATGG
NdeI 3879 CATATG CA^TATG
5515 CATATG
NspV 1003 TTCGAA TT^CGAA
3834 TTCGAA
PinAI 204 ACCGGT A^CCGGT
5792 ACCGGT
SfuI 1003 TTCGAA TT^CGAA
3834 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BalI BamHI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmnI