##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BfrI BlnI BsiWI BssHII Bst1107I
BstZ17I CpoI EagI Eam1105I EclXI
Eco47III Eco52I FspI KasI KpnI
MfeI MluI MunI NarI NdeI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I RsrII
SfiI SfoI SnaBI SpeI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 823 GACGTC GACGT^C
AcyI 823 GACGTC G[GA]^CG[CT]C
BanI 5617 GGTGCC G^G[CT][GA]CC
BsaBI 5960 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 823 GACGTC G[GA]^CG[CT]C
Bsp1407I 2156 TGTACA T^GTACA
BsrGI 2156 TGTACA T^GTACA
BssSI 9096 CACGAG CACGAG(-5/-1)
BstBI 417 TTCGAA TT^CGAA
EcoT22I 5244 ATGCAT ATGCA^T
FseI 4466 GGCCGGCC GGCCGG^CC
Hin1I 823 GACGTC G[GA]^CG[CT]C
KspI 4125 CCGCGG CCGC^GG
MamI 5960 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 5244 ATGCAT ATGCA^T
NspV 417 TTCGAA TT^CGAA
PaeR7I 8258 CTCGAG C^TCGAG
PshAI 9021 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1173 CGATCG CGAT^CG
SacII 4125 CCGCGG CCGC^GG
SalI 748 GTCGAC G^TCGAC
SbfI 8445 CCTGCAGG CCTGCA^GG
SfuI 417 TTCGAA TT^CGAA
SgrAI 5767 CACCGGCG C[GA]^CCGG[CT]G
SphI 978 GCATGC GCATG^C
Sse8387I 8445 CCTGCAGG CCTGCA^GG
SspBI 2156 TGTACA T^GTACA
XhoI 8258 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 1223 GGGCCC GGGCC^C
2077 GGGCCC
AspI 6217 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
8487 GACGAAGTC
BbvCI 5814 CCTCAGC CCTCAGC(-5/-2)
4336 GCTGAGG
BciVI 3036 GGATAC GTATCC(6/5)
1016 GTATCC
BsaAI 7804 TACGTG [CT]AC^GT[GA]
7923 CACGTA
BsmBI 5113 GAGACG CGTCTC(1/5)
7510 GAGACG
BspLU11I 8264 ACATGT A^CATGT
8672 ACATGT
BstAPI 2407 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5649 GCAGAAGCTGC
BstEII 2688 GGTAACC G^GT[ACGT]ACC
8682 GGTGACC
BstPI 2688 GGTAACC G^GT[ACGT]ACC
8682 GGTGACC
DrdI 5851 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7894 GACTTCCAGGTC
EcoO65I 2688 GGTAACC G^GT[ACGT]ACC
8682 GGTGACC
EcoRV 3214 GATATC GAT^ATC
7066 GATATC
HindIII 2134 AAGCTT A^AGCTT
8470 AAGCTT
HpaI 1473 GTTAAC GTT^AAC
2049 GTTAAC
NaeI 1231 GCCGGC GCC^GGC
4467 GCCGGC
NgoMIV 1231 GCCGGC G^CCGGC
4467 GCCGGC
NheI 790 GCTAGC G^CTAGC
3636 GCTAGC
PflFI 6217 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
8487 GACGAAGTC
SacI 4354 GAGCTC GAGCT^C
5989 GAGCTC
SapI 4266 GAAGAGC GCTCTTC(1/4)
1647 GCTCTTC
ScaI 4490 AGTACT AGT^ACT
6758 AGTACT
SspI 3652 AATATT AAT^ATT
9376 AATATT
Tth111I 6217 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
8487 GACGAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanII BbsI BbvI BcgI BclI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI