##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AgeI AhdI AscI Asp718I AspEI
AviII BamHI BbrPI BcgI BclI
BfrI BglII BsaBI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoRV FbaI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfuI
SnaBI SpeI SphI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AclI 5373 AACGTT AA^CGTT
Alw44I 3569 GTGCAC G^TGCAC
ApaLI 3569 GTGCAC G^TGCAC
AseI 5333 ATTAAT AT^TAAT
AsnI 5333 ATTAAT AT^TAAT
BbeI 1033 GGCGCC GGCGC^C
BciVI 2498 GGATAC GTATCC(6/5)
BsaAI 5724 CACGTA [CT]AC^GT[GA]
BsaWI 1511 ACCGGA [AT]^CCGG[AT]
BsmI 1218 GAATGC GAATGC(1/-1)
BspHI 4689 TCATGA T^CATGA
DraI 386 TTTAAA TTT^AAA
DraIII 231 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 5821 GAATTC G^AATTC
EcoT22I 66 ATGCAT ATGCA^T
FseI 1698 GGCCGGCC GGCCGG^CC
HindIII 358 AAGCTT A^AGCTT
KasI 1033 GGCGCC G^GCGCC
NarI 1033 GGCGCC GG^CGCC
NsiI 66 ATGCAT ATGCA^T
PaeR7I 1643 CTCGAG C^TCGAG
PshBI 5333 ATTAAT AT^TAAT
Psp1406I 5373 AACGTT AA^CGTT
RcaI 4689 TCATGA T^CATGA
SacI 4455 GAGCTC GAGCT^C
SapI 2436 GAAGAGC GCTCTTC(1/4)
SfiI 1739 GGCCCCAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1033 GGCGCC GGC^GCC
SgrAI 1786 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2007 CCCGGG CCC^GGG
XhoI 1643 CTCGAG C^TCGAG
XmaCI 2007 CCCGGG C^CCGGG
XmaI 2007 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 741 CGCG CG^CG
2442 CGCG
AcyI 1033 GGCGCC G[GA]^CG[CT]C
1995 GACGCC
Aor51HI 720 AGCGCT AGC^GCT
1392 AGCGCT
AspHI 3569 GTGCAC G[AT]GC[AT]^C
4455 GAGCTC
AspI 2050 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4660 GACAAAGTC
AvrII 3875 CCTAGG C^CTAGG
4190 CCTAGG
BlnI 3875 CCTAGG C^CTAGG
4190 CCTAGG
BsaHI 1033 GGCGCC G[GA]^CG[CT]C
1995 GACGCC
BsiHKAI 3569 GTGCAC G[AT]GC[AT]^C
4455 GAGCTC
BsmBI 5601 CGTCTC CGTCTC(1/5)
2462 GAGACG
Bsp1407I 5431 TGTACA T^GTACA
5554 TGTACA
BspMI 4889 GCAGGT ACCTGC(4/8)
4989 GCAGGT
BsrDI 1587 GCAATG GCAATG(2/0)
5760 GCAATG
BsrGI 5431 TGTACA T^GTACA
5554 TGTACA
BssSI 1497 CACGAG CACGAG(-5/-1)
4474 CACGAG
BstUI 741 CGCG CG^CG
2442 CGCG
Eco47III 720 AGCGCT AGC^GCT
1392 AGCGCT
HgaI 1995 GACGC GACGC(5/10)
5600 GCGTC
Hin1I 1033 GGCGCC G[GA]^CG[CT]C
1995 GACGCC
MvnI 741 CGCG CG^CG
2442 CGCG
NaeI 1699 GCCGGC GCC^GGC
1766 GCCGGC
NcoI 1228 CCATGG C^CATGG
3090 CCATGG
NdeI 2665 CATATG CA^TATG
5502 CATATG
NgoMIV 1699 GCCGGC G^CCGGC
1766 GCCGGC
PflFI 2050 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4660 GACAAAGTC
PshAI 990 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1845 GACCAAGGTC
PstI 526 CTGCAG CTGCA^G
830 CTGCAG
SfcI 526 CTGCAG C^T[GA][CT]AG
830 CTGCAG
SspBI 5431 TGTACA T^GTACA
5554 TGTACA
SspI 5260 AATATT AAT^ATT
5325 AATATT
StuI 1130 AGGCCT AGG^CCT
3505 AGGCCT
Tth111I 2050 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4660 GACAAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI