##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII AgeI AocI
AscI AseI AsnI Asp718I BfrI
BsaBI BsiEI BsiWI BsmBI BspHI
BssHII Bst1107I BstEII BstPI BstZ17I
Bsu36I CpoI EagI EarI EclXI
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI HincII HindII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MunI NotI NsiI PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SfiI
SgrAI SnaBI SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 32 GACGTC GACGT^C
AccI 679 GTCTAC GT^[AC][GT]AC
Alw44I 349 GTGCAC G^TGCAC
Aor51HI 1202 AGCGCT AGC^GCT
ApaI 1817 GGGCCC GGGCC^C
ApaLI 349 GTGCAC G^TGCAC
Asp700I 3949 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5292 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 491 TGCGCA TGC^GCA
BbrPI 3326 CACGTG CAC^GTG
BclI 4338 TGATCA T^GATCA
BglI 1810 GCCTTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3326 CACGTG [CT]AC^GT[GA]
Bsp1407I 2965 TGTACA T^GTACA
BspDI 4535 ATCGAT AT^CGAT
BsrBI 4706 GAGCGG CCGCTC(-3/-3)
BsrGI 2965 TGTACA T^GTACA
BssSI 2403 CACGAG CACGAG(-5/-1)
BstBI 1601 TTCGAA TT^CGAA
ClaI 4535 ATCGAT AT^CGAT
DraI 2075 TTTAAA TTT^AAA
DrdI 3053 GACACCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1202 AGCGCT AGC^GCT
EcoRI 4041 GAATTC G^AATTC
FbaI 4338 TGATCA T^GATCA
FspI 491 TGCGCA TGC^GCA
HgaI 1701 GACGC GACGC(5/10)
HindIII 60 AAGCTT A^AGCTT
NaeI 237 GCCGGC GCC^GGC
NgoMIV 237 GCCGGC G^CCGGC
NheI 4284 GCTAGC G^CTAGC
NruI 81 TCGCGA TCG^CGA
NspV 1601 TTCGAA TT^CGAA
PaeR7I 2950 CTCGAG C^TCGAG
PflFI 5292 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3326 CACGTG CAC^GTG
PmlI 3326 CACGTG CAC^GTG
PshAI 3519 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 5797 CCTGCAGG CCTGCA^GG
SfuI 1601 TTCGAA TT^CGAA
Sse8387I 5797 CCTGCAGG CCTGCA^GG
SspBI 2965 TGTACA T^GTACA
SspI 2105 AATATT AAT^ATT
Tth111I 5292 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3515 TCTAGA T^CTAGA
XhoI 2950 CTCGAG C^TCGAG
XmnI 3949 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 219 TCCGGA T^CCGGA
4321 TCCGGA
AhdI 3138 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4385 GACCCTCCGTC
AspEI 3138 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4385 GACCCTCCGTC
AvrII 3886 CCTAGG C^CTAGG
4649 CCTAGG
BbeI 128 GGCGCC GGCGC^C
179 GGCGCC
BbvCI 655 GCTGAGG CCTCAGC(-5/-2)
4374 GCTGAGG
BcgI 2952 CGAGGGTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2989 CGATGTCCTTGC
BciVI 5977 GGATAC GTATCC(6/5)
2051 GTATCC
BlnI 3886 CCTAGG C^CTAGG
4649 CCTAGG
BseAI 219 TCCGGA T^CCGGA
4321 TCCGGA
BsmI 1378 GCATTC GAATGC(1/-1)
2344 GCATTC
BspEI 219 TCCGGA T^CCGGA
4321 TCCGGA
BspLU11I 2839 ACATGT A^CATGT
5034 ACATGT
BsrFI 131 GCCGGT [GA]^CCGG[CT]
237 GCCGGC
Cfr10I 131 GCCGGT [GA]^CCGG[CT]
237 GCCGGC
Eam1105I 3138 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4385 GACCCTCCGTC
KasI 128 GGCGCC G^GCGCC
179 GGCGCC
MroI 219 TCCGGA T^CCGGA
4321 TCCGGA
NarI 128 GGCGCC GG^CGCC
179 GGCGCC
NdeI 2626 CATATG CA^TATG
3028 CATATG
NspI 2839 ACATGT [GA]CATG^[CT]
5034 ACATGT
ScaI 1015 AGTACT AGT^ACT
2859 AGTACT
SfoI 128 GGCGCC GGC^GCC
179 GGCGCC
SmaI 3227 CCCGGG CCC^GGG
5229 CCCGGG
XmaCI 3227 CCCGGG C^CCGGG
5229 CCCGGG
XmaI 3227 CCCGGG C^CCGGG
5229 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII