##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AhdI AocI AscI
Asp718I AspEI AspI AviII BbeI
BbrPI BlpI Bpu1102I BsaAI BseAI
BsiWI BsmBI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI EagI Eam1105I EclXI Eco52I
Eco81I FseI FspI HpaI KasI
KpnI KspI MluI MroI NaeI
NarI NgoMIV NotI NruI NspV
PaeR7I PflFI PmaCI PmeI PmlI
PvuI SacII SalI SfiI SfoI
SfuI SnaBI SphI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 10175 GACGTC GACGT^C
AccI 10178 GTCTAC GT^[AC][GT]AC
AccII 132 CGCG CG^CG
AclI 5543 AACGTT AA^CGTT
AcyI 10175 GACGTC G[GA]^CG[CT]C
AgeI 395 ACCGGT A^CCGGT
Alw44I 5790 GTGCAC G^TGCAC
Aor51HI 6235 AGCGCT AGC^GCT
ApaI 1303 GGGCCC GGGCC^C
ApaLI 5790 GTGCAC G^TGCAC
BalI 6900 TGGCCA TGG^CCA
BglI 1980 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 10175 GACGTC G[GA]^CG[CT]C
BsiEI 128 CGACCG CG[GA][CT]^CG
Bsp1407I 8118 TGTACA T^GTACA
BsrBI 7721 CCGCTC CCGCTC(-3/-3)
BsrFI 395 ACCGGT [GA]^CCGG[CT]
BsrGI 8118 TGTACA T^GTACA
BssSI 8175 CTCGTG CACGAG(-5/-1)
BstEII 7812 GGTAACC G^GT[ACGT]ACC
BstPI 7812 GGTAACC G^GT[ACGT]ACC
BstUI 132 CGCG CG^CG
Cfr10I 395 ACCGGT [GA]^CCGG[CT]
CpoI 4565 CGGACCG CG^G[AT]CCG
DraIII 10297 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 6235 AGCGCT AGC^GCT
EcoO65I 7812 GGTAACC G^GT[ACGT]ACC
EcoRI 2940 GAATTC G^AATTC
EcoRV 3306 GATATC GAT^ATC
Hin1I 10175 GACGTC G[GA]^CG[CT]C
MluNI 6900 TGGCCA TGG^CCA
MscI 6900 TGGCCA TGG^CCA
MvnI 132 CGCG CG^CG
PacI 8004 TTAATTAA TTAAT^TAA
PinAI 395 ACCGGT A^CCGGT
Psp1406I 5543 AACGTT AA^CGTT
RsrII 4565 CGGACCG CG^G[AT]CCG
SacI 9411 GAGCTC GAGCT^C
SbfI 627 CCTGCAGG CCTGCA^GG
ScaI 6057 AGTACT AGT^ACT
SexAI 2084 ACCAGGT A^CC[AT]GGT
SgrAI 394 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2675 CCCGGG CCC^GGG
Sse8387I 627 CCTGCAGG CCTGCA^GG
SspBI 8118 TGTACA T^GTACA
XbaI 365 TCTAGA T^CTAGA
XmaCI 2675 CCCGGG C^CCGGG
XmaI 2675 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvrII 857 CCTAGG C^CTAGG
6731 CCTAGG
BamHI 929 GGATCC G^GATCC
4292 GGATCC
BbvCI 277 CCTCAGC CCTCAGC(-5/-2)
9313 CCTCAGC
BcgI 506 CGAACATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
665 GCAGCCAAGTCG
BlnI 857 CCTAGG C^CTAGG
6731 CCTAGG
BsaWI 395 ACCGGT [AT]^CCGG[AT]
3464 ACCGGA
BspHI 2573 TCATGA T^CATGA
4243 TCATGA
BstXI 4031 CCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5130 CCACTTATGTGG
DrdI 5395 GACTCTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7023 GACCTCAGAGTC
EaeI 3968 TGGCCG [CT]^GGCC[GA]
6900 TGGCCA
EcoT22I 5531 ATGCAT ATGCA^T
6200 ATGCAT
HaeII 144 AGCGCC [GA]GCGC^[CT]
6235 AGCGCT
NsiI 5531 ATGCAT ATGCA^T
6200 ATGCAT
PshAI 1617 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7845 GACCTTGGTC
RcaI 2573 TCATGA T^CATGA
4243 TCATGA
SapI 2620 GCTCTTC GCTCTTC(1/4)
3814 GCTCTTC
SpeI 2727 ACTAGT A^CTAGT
9873 ACTAGT
SwaI 2314 ATTTAAAT ATTT^AAAT
5747 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BciVI
BclI BcnI BfaI BfrI BglII
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NcoI NdeI NdeII
NheI NlaIII NlaIV NspI PflMI
PleI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI