################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## Acc65I AccIII AhdI AocI AscI Asp718I AspEI AspI AviII BbeI BbrPI BlpI Bpu1102I BsaAI BseAI BsiWI BsmBI BspDI BspEI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI EagI Eam1105I EclXI Eco52I Eco81I FseI FspI HpaI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NotI NruI NspV PaeR7I PflFI PmaCI PmeI PmlI PvuI SacII SalI SfiI SfoI SfuI SnaBI SphI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 10175 GACGTC GACGT^C AccI 10178 GTCTAC GT^[AC][GT]AC AccII 132 CGCG CG^CG AclI 5543 AACGTT AA^CGTT AcyI 10175 GACGTC G[GA]^CG[CT]C AgeI 395 ACCGGT A^CCGGT Alw44I 5790 GTGCAC G^TGCAC Aor51HI 6235 AGCGCT AGC^GCT ApaI 1303 GGGCCC GGGCC^C ApaLI 5790 GTGCAC G^TGCAC BalI 6900 TGGCCA TGG^CCA BglI 1980 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 10175 GACGTC G[GA]^CG[CT]C BsiEI 128 CGACCG CG[GA][CT]^CG Bsp1407I 8118 TGTACA T^GTACA BsrBI 7721 CCGCTC CCGCTC(-3/-3) BsrFI 395 ACCGGT [GA]^CCGG[CT] BsrGI 8118 TGTACA T^GTACA BssSI 8175 CTCGTG CACGAG(-5/-1) BstEII 7812 GGTAACC G^GT[ACGT]ACC BstPI 7812 GGTAACC G^GT[ACGT]ACC BstUI 132 CGCG CG^CG Cfr10I 395 ACCGGT [GA]^CCGG[CT] CpoI 4565 CGGACCG CG^G[AT]CCG DraIII 10297 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 6235 AGCGCT AGC^GCT EcoO65I 7812 GGTAACC G^GT[ACGT]ACC EcoRI 2940 GAATTC G^AATTC EcoRV 3306 GATATC GAT^ATC Hin1I 10175 GACGTC G[GA]^CG[CT]C MluNI 6900 TGGCCA TGG^CCA MscI 6900 TGGCCA TGG^CCA MvnI 132 CGCG CG^CG PacI 8004 TTAATTAA TTAAT^TAA PinAI 395 ACCGGT A^CCGGT Psp1406I 5543 AACGTT AA^CGTT RsrII 4565 CGGACCG CG^G[AT]CCG SacI 9411 GAGCTC GAGCT^C SbfI 627 CCTGCAGG CCTGCA^GG ScaI 6057 AGTACT AGT^ACT SexAI 2084 ACCAGGT A^CC[AT]GGT SgrAI 394 CACCGGTG C[GA]^CCGG[CT]G SmaI 2675 CCCGGG CCC^GGG Sse8387I 627 CCTGCAGG CCTGCA^GG SspBI 8118 TGTACA T^GTACA XbaI 365 TCTAGA T^CTAGA XmaCI 2675 CCCGGG C^CCGGG XmaI 2675 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AvrII 857 CCTAGG C^CTAGG 6731 CCTAGG BamHI 929 GGATCC G^GATCC 4292 GGATCC BbvCI 277 CCTCAGC CCTCAGC(-5/-2) 9313 CCTCAGC BcgI 506 CGAACATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 665 GCAGCCAAGTCG BlnI 857 CCTAGG C^CTAGG 6731 CCTAGG BsaWI 395 ACCGGT [AT]^CCGG[AT] 3464 ACCGGA BspHI 2573 TCATGA T^CATGA 4243 TCATGA BstXI 4031 CCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5130 CCACTTATGTGG DrdI 5395 GACTCTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7023 GACCTCAGAGTC EaeI 3968 TGGCCG [CT]^GGCC[GA] 6900 TGGCCA EcoT22I 5531 ATGCAT ATGCA^T 6200 ATGCAT HaeII 144 AGCGCC [GA]GCGC^[CT] 6235 AGCGCT NsiI 5531 ATGCAT ATGCA^T 6200 ATGCAT PshAI 1617 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 7845 GACCTTGGTC RcaI 2573 TCATGA T^CATGA 4243 TCATGA SapI 2620 GCTCTTC GCTCTTC(1/4) 3814 GCTCTTC SpeI 2727 ACTAGT A^CTAGT 9873 ACTAGT SwaI 2314 ATTTAAAT ATTT^AAAT 5747 ATTTAAAT ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BfrI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NcoI NdeI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI