##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PstI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 877 CGCG CG^CG
AclI 1177 AACGTT AA^CGTT
AflII 182 CTTAAG C^TTAAG
Alw44I 2016 GTGCAC G^TGCAC
AlwNI 102 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 689 CCTGAGG CC^T[ACGT]AGG
ApaLI 2016 GTGCAC G^TGCAC
BalI 1226 TGGCCA TGG^CCA
BanII 882 GAGCTC G[GA]GC[CT]^C
BbsI 1643 GAAGAC GAAGAC(2/6)
BcnI 1648 CCGGG CC^[GC]GG
BfrI 182 CTTAAG C^TTAAG
BglII 738 AGATCT A^GATCT
BpuAI 1643 GAAGAC GAAGAC(2/6)
BsaI 1020 GGTCTC GGTCTC(1/5)
BseRI 507 GAGGAG GAGGAG(10/8)
BsmAI 1021 GTCTC GTCTC(1/5)
BsmI 2828 GCATTC GAATGC(1/-1)
Bsp1407I 810 TGTACA T^GTACA
BsrGI 810 TGTACA T^GTACA
BstEII 1592 GGTCACC G^GT[ACGT]ACC
BstPI 1592 GGTCACC G^GT[ACGT]ACC
BstUI 877 CGCG CG^CG
Bsu36I 689 CCTGAGG CC^T[ACGT]AGG
DraII 2488 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 1226 TGGCCA [CT]^GGCC[GA]
Eco81I 689 CCTGAGG CC^T[ACGT]AGG
EcoO109I 2488 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1592 GGTCACC G^GT[ACGT]ACC
EcoRI 2364 GAATTC G^AATTC
EcoRV 3916 GATATC GAT^ATC
FauI 878 GCGGG CCCGC(4/6)
HapII 1648 CCGG C^CGG
HincII 1004 GTTAAC GT[CT]^[GA]AC
HindII 1004 GTTAAC GT[CT]^[GA]AC
HpaI 1004 GTTAAC GTT^AAC
HpaII 1648 CCGG C^CGG
MluNI 1226 TGGCCA TGG^CCA
MscI 1226 TGGCCA TGG^CCA
MspI 1648 CCGG C^CGG
MvnI 877 CGCG CG^CG
NciI 1648 CCGGG CC^[GC]GG
PflMI 207 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2488 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1177 AACGTT AA^CGTT
SacI 882 GAGCTC GAGCT^C
SapI 3487 GAAGAGC GCTCTTC(1/4)
ScaI 1386 AGTACT AGT^ACT
SspBI 810 TGTACA T^GTACA
SwaI 3936 ATTTAAAT ATTT^AAAT
Van91I 207 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 93 CCACCTACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AspHI 882 GAGCTC G[AT]GC[AT]^C
2016 GTGCAC
BbvCI 1785 CCTCAGC CCTCAGC(-5/-2)
1872 GCTGAGG
BmyI 882 GAGCTC G[AGT]GC[ACT]^C
2016 GTGCAC
BpmI 628 CTGGAG CTGGAG(16/14)
1965 CTGGAG
BsiHKAI 882 GAGCTC G[AT]GC[AT]^C
2016 GTGCAC
Bsp1286I 882 GAGCTC G[AGT]GC[ACT]^C
2016 GTGCAC
BstXI 1096 CCACCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1155 CCAGCTGCCTGG
DsaI 1032 CCATGG C^C[GA][CT]GG
3048 CCATGG
EarI 650 GAAGAG CTCTTC(1/4)
3487 GAAGAG
HindIII 1838 AAGCTT A^AGCTT
2347 AAGCTT
Ksp632I 650 GAAGAG CTCTTC(1/4)
3487 GAAGAG
NcoI 1032 CCATGG C^CATGG
3048 CCATGG
SphI 2605 GCATGC GCATG^C
3785 GCATGC
SspI 3327 AATATT AAT^ATT
3665 AATATT
StuI 186 AGGCCT AGG^CCT
1308 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AvaII BbvI BclI BfaI BsaJI
BsgI BsiYI BslI BsmFI BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI