##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BbeI BcgI BciVI BclI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 921 GTCTAC GT^[AC][GT]AC
AflII 2115 CTTAAG C^TTAAG
BanII 2024 GAGCCC G[GA]GC[CT]^C
BbrPI 415 CACGTG CAC^GTG
BcnI 1949 CCCGG CC^[GC]GG
BfrI 2115 CTTAAG C^TTAAG
BglII 2781 AGATCT A^GATCT
BsaAI 415 CACGTG [CT]AC^GT[GA]
BsaWI 1231 ACCGGA [AT]^CCGG[AT]
BspLU11I 2922 ACATGT A^CATGT
BssSI 116 CACGAG CACGAG(-5/-1)
BstXI 387 CCACACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2781 AGATCT [GA]^GATC[CT]
CfoI 748 GCGC GCG^C
DraIII 116 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2383 GACCCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 356 CCATGG C^C[GA][CT]GG
EcoT22I 3035 ATGCAT ATGCA^T
HgaI 2515 GCGTC GACGC(5/10)
HhaI 748 GCGC GCG^C
HinP1I 748 GCGC G^CGC
HincII 1119 GTCAAC GT[CT]^[GA]AC
HindII 1119 GTCAAC GT[CT]^[GA]AC
HindIII 1763 AAGCTT A^AGCTT
MfeI 1010 CAATTG C^AATTG
MflI 2781 AGATCT [GA]^GATC[CT]
MunI 1010 CAATTG C^AATTG
NciI 1949 CCCGG CC^[GC]GG
NcoI 356 CCATGG C^CATGG
NdeI 878 CATATG CA^TATG
NsiI 3035 ATGCAT ATGCA^T
PflMI 2014 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 415 CACGTG CAC^GTG
PmlI 415 CACGTG CAC^GTG
PstI 217 CTGCAG CTGCA^G
SexAI 1920 ACCTGGT A^CC[AT]GGT
SmlI 2115 CTTAAG C^T[CT][GA]AG
SpeI 1843 ACTAGT A^CTAGT
TaqI 2505 TCGA T^CGA
Tsp45I 1264 GTGAC ^GT[GC]AC
TthHB8I 2505 TCGA T^CGA
Van91I 2014 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1904 CCAGTACACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2781 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 1579 ACACGT A^C[GA][CT]GT
2922 ACATGT
AlwNI 1025 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1271 CAGCCTCTG
AseI 733 ATTAAT AT^TAAT
790 ATTAAT
AsnI 733 ATTAAT AT^TAAT
790 ATTAAT
AvaII 2382 GGACC G^G[AT]CC
3186 GGACC
BalI 2046 TGGCCA TGG^CCA
2121 TGGCCA
BbsI 1084 GAAGAC GAAGAC(2/6)
2521 GTCTTC
BbvCI 211 CCTCAGC CCTCAGC(-5/-2)
1268 CCTCAGC
BpuAI 1084 GAAGAC GAAGAC(2/6)
2521 GTCTTC
BsmFI 664 GGGAC GGGAC(10/14)
1698 GTCCC
BsmI 2178 GAATGC GAATGC(1/-1)
2054 GCATTC
Bsp1407I 3070 TGTACA T^GTACA
3111 TGTACA
BspMI 744 ACCTGC ACCTGC(4/8)
1515 ACCTGC
BsrDI 866 CATTGC GCAATG(2/0)
719 GCAATG
BsrGI 3070 TGTACA T^GTACA
3111 TGTACA
DraI 2600 TTTAAA TTT^AAA
2832 TTTAAA
EaeI 2046 TGGCCA [CT]^GGCC[GA]
2121 TGGCCA
EcoT14I 356 CCATGG C^C[AT][AT]GG
2049 CCAAGG
HapII 1232 CCGG C^CGG
1950 CCGG
HpaII 1232 CCGG C^CGG
1950 CCGG
MluNI 2046 TGGCCA TGG^CCA
2121 TGGCCA
MscI 2046 TGGCCA TGG^CCA
2121 TGGCCA
MspI 1232 CCGG C^CGG
1950 CCGG
NheI 340 GCTAGC G^CTAGC
2897 GCTAGC
PshBI 733 ATTAAT AT^TAAT
790 ATTAAT
ScaI 475 AGTACT AGT^ACT
909 AGTACT
SspBI 3070 TGTACA T^GTACA
3111 TGTACA
SspI 556 AATATT AAT^ATT
1168 AATATT
StyI 356 CCATGG C^C[AT][AT]GG
2049 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AcsI AfaI AluI ApoI BanI
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI