##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccII AccIII Aor51HI ApaI
AscI Asp718I AvrII BbeI BbrPI
BcgI BciVI BglI BlnI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BssHII
BstAPI BstUI CelII ClaI CpoI
EagI EclXI Eco47III Eco52I EcoNI
FauI FseI HpaI KasI KpnI
KspI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AclI 2639 AACGTT AA^CGTT
AflII 1240 CTTAAG C^TTAAG
AflIII 6667 ACATGT A^C[GA][CT]GT
AgeI 4449 ACCGGT A^CCGGT
AhdI 3496 GACTGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3242 GTGCAC G^TGCAC
ApaLI 3242 GTGCAC G^TGCAC
AspEI 3496 GACTGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2499 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3876 TGCGCA TGC^GCA
BalI 3905 TGGCCA TGG^CCA
BamHI 2573 GGATCC G^GATCC
BbvCI 5295 CCTCAGC CCTCAGC(-5/-2)
BfrI 1240 CTTAAG C^TTAAG
BsaWI 4449 ACCGGT [AT]^CCGG[AT]
BspLU11I 6667 ACATGT A^CATGT
BsrFI 4449 ACCGGT [GA]^CCGG[CT]
BstBI 1554 TTCGAA TT^CGAA
Cfr10I 4449 ACCGGT [GA]^CCGG[CT]
DrdI 1774 GACGTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4149 CCATGG C^C[GA][CT]GG
EaeI 3905 TGGCCA [CT]^GGCC[GA]
Eam1105I 3496 GACTGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 511 GATATC GAT^ATC
EcoT22I 5730 ATGCAT ATGCA^T
FspI 3876 TGCGCA TGC^GCA
HaeII 426 AGCGCC [GA]GCGC^[CT]
HgaI 3281 GACGC GACGC(5/10)
MluNI 3905 TGGCCA TGG^CCA
MscI 3905 TGGCCA TGG^CCA
MspA1I 2619 CAGCTG C[AC]G^C[GT]G
NcoI 4149 CCATGG C^CATGG
NdeI 5219 CATATG CA^TATG
NsiI 5730 ATGCAT ATGCA^T
NspV 1554 TTCGAA TT^CGAA
PacI 4790 TTAATTAA TTAAT^TAA
PaeR7I 462 CTCGAG C^TCGAG
PflFI 2499 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4449 ACCGGT A^CCGGT
PpuMI 4273 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2546 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2639 AACGTT AA^CGTT
PvuII 2619 CAGCTG CAG^CTG
SacI 727 GAGCTC GAGCT^C
SexAI 3264 ACCAGGT A^CC[AT]GGT
SfuI 1554 TTCGAA TT^CGAA
SmaI 4269 CCCGGG CCC^GGG
SphI 5494 GCATGC GCATG^C
StuI 1566 AGGCCT AGG^CCT
Tth111I 2499 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 595 TCTAGA T^CTAGA
XcmI 2984 CCATTGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 462 CTCGAG C^TCGAG
XmaCI 4269 CCCGGG C^CCGGG
XmaI 4269 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 167 GACGTC GACGT^C
1774 GACGTC
AciI 2032 GCGG CCGC(-3/-1)
2035 GCGG
AcyI 167 GACGTC G[GA]^CG[CT]C
1774 GACGTC
AlwNI 425 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
849 CAGCATCTG
AseI 4793 ATTAAT AT^TAAT
5982 ATTAAT
AsnI 4793 ATTAAT AT^TAAT
5982 ATTAAT
Asp700I 2745 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6316 GAAGAGTTTC
AspHI 727 GAGCTC G[AT]GC[AT]^C
3242 GTGCAC
BanI 3917 GGTGCC G^G[CT][GA]CC
5412 GGTGCC
BclI 318 TGATCA T^GATCA
3592 TGATCA
BsaBI 771 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1331 GATCTTGATC
BsaHI 167 GACGTC G[GA]^CG[CT]C
1774 GACGTC
BsiHKAI 727 GAGCTC G[AT]GC[AT]^C
3242 GTGCAC
BsmI 3076 GAATGC GAATGC(1/-1)
5765 GCATTC
BssSI 1381 CACGAG CACGAG(-5/-1)
3543 CTCGTG
BstEII 4936 GGTAACC G^GT[ACGT]ACC
6269 GGTAACC
BstPI 4936 GGTAACC G^GT[ACGT]ACC
6269 GGTAACC
DraII 4273 GGGACCC [GA]G^G[ACGT]CC[CT]
4333 AGGCCCC
DraIII 2722 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4629 CACATTGTG
EcoO109I 4273 GGGACCC [GA]G^G[ACGT]CC[CT]
4333 AGGCCCC
EcoO65I 4936 GGTAACC G^GT[ACGT]ACC
6269 GGTAACC
EcoRI 700 GAATTC G^AATTC
4586 GAATTC
FbaI 318 TGATCA T^GATCA
3592 TGATCA
Hin1I 167 GACGTC G[GA]^CG[CT]C
1774 GACGTC
MamI 771 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1331 GATCTTGATC
PshBI 4793 ATTAAT AT^TAAT
5982 ATTAAT
XmnI 2745 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6316 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AocI ApoI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstXI BstYI
BstZ17I Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI Eco81I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspI MunI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RcaI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII