##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I ApaLI AscI
Asp700I Asp718I AspEI AspI AvrII
BamHI BcgI BclI BfrI BglII
BlnI BsaBI BseAI BsgI BsiWI
BsmI BspDI BspEI BspHI BsrBI
BsrDI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
Eam1105I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NcoI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tth111I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 671 GACGTC GACGT^C
AlwI 310 GATCC GGATC(4/5)
AocI 1717 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1908 AGCGCT AGC^GCT
AseI 2132 ATTAAT AT^TAAT
AsnI 2132 ATTAAT AT^TAAT
AviII 612 TGCGCA TGC^GCA
BalI 297 TGGCCA TGG^CCA
BbrPI 1562 CACGTG CAC^GTG
BbsI 260 GTCTTC GAAGAC(2/6)
BbvCI 1756 CCTCAGC CCTCAGC(-5/-2)
BciVI 1241 GTATCC GTATCC(6/5)
BfaI 1546 CTAG C^TAG
BpuAI 260 GTCTTC GAAGAC(2/6)
BsaAI 1562 CACGTG [CT]AC^GT[GA]
BsaWI 1800 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2048 TGTACA T^GTACA
BspLU11I 2435 ACATGT A^CATGT
BspMI 684 ACCTGC ACCTGC(4/8)
BsrGI 2048 TGTACA T^GTACA
BstAPI 1455 GCATGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 309 AGATCC [GA]^GATC[CT]
Bsu36I 1717 CCTCAGG CC^T[ACGT]AGG
DraIII 1559 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1460 GACTGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1448 CTCTTC CTCTTC(1/4)
Eco47III 1908 AGCGCT AGC^GCT
Eco81I 1717 CCTCAGG CC^T[ACGT]AGG
EcoT14I 465 CCAAGG C^C[AT][AT]GG
FseI 754 GGCCGGCC GGCCGG^CC
FspI 612 TGCGCA TGC^GCA
HinfI 1322 GACTC G^A[ACGT]TC
Ksp632I 1448 CTCTTC CTCTTC(1/4)
MaeI 1546 CTAG C^TAG
MflI 309 AGATCC [GA]^GATC[CT]
MluNI 297 TGGCCA TGG^CCA
MscI 297 TGGCCA TGG^CCA
NspI 2435 ACATGT [GA]CATG^[CT]
PleI 1322 GACTC GAGTC(4/5)
PmaCI 1562 CACGTG CAC^GTG
PmlI 1562 CACGTG CAC^GTG
PshBI 2132 ATTAAT AT^TAAT
SacI 1169 GAGCTC GAGCT^C
SfcI 862 CTACAG C^T[GA][CT]AG
SgrAI 1090 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 2048 TGTACA T^GTACA
StuI 801 AGGCCT AGG^CCT
StyI 465 CCAAGG C^C[AT][AT]GG
XhoII 309 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 2049 GTAC GT^AC
2252 GTAC
AflIII 27 ACGTGT A^C[GA][CT]GT
2435 ACATGT
AlwNI 703 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1048 CAGCGGCTG
AspHI 158 GAGCAC G[AT]GC[AT]^C
1169 GAGCTC
BlpI 1911 GCTCAGC GC^T[ACGT]AGC
2351 GCTGAGC
BpmI 1861 CTCCAG CTGGAG(16/14)
1166 CTGGAG
Bpu1102I 1911 GCTCAGC GC^T[ACGT]AGC
2351 GCTGAGC
BsaI 1734 GAGACC GGTCTC(1/5)
1970 GGTCTC
BsiHKAI 158 GAGCAC G[AT]GC[AT]^C
1169 GAGCTC
BsmBI 1604 GAGACG CGTCTC(1/5)
1728 GAGACG
CelII 1911 GCTCAGC GC^T[ACGT]AGC
2351 GCTGAGC
HincII 122 GTCAAC GT[CT]^[GA]AC
1064 GTCAAC
HindII 122 GTCAAC GT[CT]^[GA]AC
1064 GTCAAC
MboII 261 TCTTC GAAGA(8/7)
1449 TCTTC
MslI 418 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
553 CACTGGGATG
NotI 695 GCGGCCGC GC^GGCCGC
1298 GCGGCCGC
RsaI 2049 GTAC GT^AC
2252 GTAC
SfiI 202 GGCCCTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1519 GGCCACCCAGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
ApaI ApoI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI KspI MaeII MaeIII
MboI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PpuMI SacII Sau3AI Sau96I
ScrFI SfaNI SfoI SmaI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XmaCI XmaI